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Plasma beta-hexosaminidase isoenzymes A and B exhibit different relations to blood glucose levels in a population of Type 1 diabetic patients

Hultberg, Björn LU ; Isaksson, Anders LU ; Agardh, Elisabet LU and Agardh, Carl-David LU (1995) In Scandinavian Journal of Clinical & Laboratory Investigation 55(8). p.723-728
Abstract
The activity of lysosomal enzymes, such as beta-hexosaminidase (Hex), is increased in the plasma and serum of diabetic patients. A positive association has been shown between enzyme activity and glycated proteins, indicating an association with the degree of metabolic control. Several isoenzymes of Hex exist. Studies have reported different proportions of the isoenzymes in plasma from diabetic patients, compared with healthy subjects. In the present study, Hex isoenzymes were examined in 76 Type 1 diabetic patients, of mean age 37.4 years (SD 12.9) compared with 38 age- and sex-matched healthy control subjects in an attempt to evaluate the influence of long- and short-term changes in blood glucose levels on these isoenzymes. The results... (More)
The activity of lysosomal enzymes, such as beta-hexosaminidase (Hex), is increased in the plasma and serum of diabetic patients. A positive association has been shown between enzyme activity and glycated proteins, indicating an association with the degree of metabolic control. Several isoenzymes of Hex exist. Studies have reported different proportions of the isoenzymes in plasma from diabetic patients, compared with healthy subjects. In the present study, Hex isoenzymes were examined in 76 Type 1 diabetic patients, of mean age 37.4 years (SD 12.9) compared with 38 age- and sex-matched healthy control subjects in an attempt to evaluate the influence of long- and short-term changes in blood glucose levels on these isoenzymes. The results show that Hex A activity (p<0.01), but not Hex B activity, was higher in the diabetic patients. Hex A activity was positively associated with both the actual blood glucose levels (r = 0.48, p<0.001) and haemoglobin A1c (HbA1c) (r = 0.43, p<0.001), while Hex B activity was associated with the level of HbA1c only (r = 0.42, p<0.001). Both Hex A and B activities were also positively associated with early signs of diabetic nephropathy (e.g. urinary excretion of Hex, fractional albumin excretion ratio and urinary albumin). There was no association between Hex A and B activities and different degrees of retinopathy. In conclusion, the present study demonstrates an association between Hex A and B and metabolic parameters in diabetes as well as with clinical signs of early diabetic nephropathy, but no association with the degree of retinopathy. Furthermore, Hex A seems to be more influenced than Hex B by short-term changes in blood glucose levels. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
β-hexosaminidase, blood glucose, diabetes mellitus, glycated haemoglobin, isoenzymes, nephropathy, plasma, retinopathy
in
Scandinavian Journal of Clinical & Laboratory Investigation
volume
55
issue
8
pages
723 - 728
publisher
Informa Healthcare
external identifiers
  • pmid:8903842
  • scopus:0029417070
ISSN
1502-7686
DOI
10.3109/00365519509075402
language
English
LU publication?
yes
id
4fcd760f-0d8b-4674-bf55-140a6a30a8f5 (old id 1109150)
date added to LUP
2008-07-25 14:26:06
date last changed
2017-01-01 06:59:09
@article{4fcd760f-0d8b-4674-bf55-140a6a30a8f5,
  abstract     = {The activity of lysosomal enzymes, such as beta-hexosaminidase (Hex), is increased in the plasma and serum of diabetic patients. A positive association has been shown between enzyme activity and glycated proteins, indicating an association with the degree of metabolic control. Several isoenzymes of Hex exist. Studies have reported different proportions of the isoenzymes in plasma from diabetic patients, compared with healthy subjects. In the present study, Hex isoenzymes were examined in 76 Type 1 diabetic patients, of mean age 37.4 years (SD 12.9) compared with 38 age- and sex-matched healthy control subjects in an attempt to evaluate the influence of long- and short-term changes in blood glucose levels on these isoenzymes. The results show that Hex A activity (p&lt;0.01), but not Hex B activity, was higher in the diabetic patients. Hex A activity was positively associated with both the actual blood glucose levels (r = 0.48, p&lt;0.001) and haemoglobin A1c (HbA1c) (r = 0.43, p&lt;0.001), while Hex B activity was associated with the level of HbA1c only (r = 0.42, p&lt;0.001). Both Hex A and B activities were also positively associated with early signs of diabetic nephropathy (e.g. urinary excretion of Hex, fractional albumin excretion ratio and urinary albumin). There was no association between Hex A and B activities and different degrees of retinopathy. In conclusion, the present study demonstrates an association between Hex A and B and metabolic parameters in diabetes as well as with clinical signs of early diabetic nephropathy, but no association with the degree of retinopathy. Furthermore, Hex A seems to be more influenced than Hex B by short-term changes in blood glucose levels.},
  author       = {Hultberg, Björn and Isaksson, Anders and Agardh, Elisabet and Agardh, Carl-David},
  issn         = {1502-7686},
  keyword      = {β-hexosaminidase,blood glucose,diabetes mellitus,glycated haemoglobin,isoenzymes,nephropathy,plasma,retinopathy},
  language     = {eng},
  number       = {8},
  pages        = {723--728},
  publisher    = {Informa Healthcare},
  series       = {Scandinavian Journal of Clinical & Laboratory Investigation},
  title        = {Plasma beta-hexosaminidase isoenzymes A and B exhibit different relations to blood glucose levels in a population of Type 1 diabetic patients},
  url          = {http://dx.doi.org/10.3109/00365519509075402},
  volume       = {55},
  year         = {1995},
}