Species-specific identification of methicillin resistance in staphylococci
(1995) In European Journal of Clinical Microbiology & Infectious Diseases 14(3). p.206-211- Abstract
- The ability to identify methicillin-resistant staphylococci by the disc diffusion method was evaluated using discs containing oxacillin (1, 5 and 10 micrograms), methicillin (10 micrograms) and cephalexin (30 micrograms). Strains of Staphylococcus aureus (67 strains) and coagulase-negative staphylococci (72 novobiocin-sensitive and 27 novobiocin-resistant strains) were studied using two inoculum densities (10(6) cfu/ml and 10(8) cfu/ml). Inhibitory zones were recorded after 18, 24 and 42 hours of incubation. A mecA-specific application of the polymerase chain reaction was used as a reference method. The inoculum of 10(8) cfu/ml and incubation for 24 hours were optimal for the identification of methicillin-resistant strains. However, one... (More)
- The ability to identify methicillin-resistant staphylococci by the disc diffusion method was evaluated using discs containing oxacillin (1, 5 and 10 micrograms), methicillin (10 micrograms) and cephalexin (30 micrograms). Strains of Staphylococcus aureus (67 strains) and coagulase-negative staphylococci (72 novobiocin-sensitive and 27 novobiocin-resistant strains) were studied using two inoculum densities (10(6) cfu/ml and 10(8) cfu/ml). Inhibitory zones were recorded after 18, 24 and 42 hours of incubation. A mecA-specific application of the polymerase chain reaction was used as a reference method. The inoculum of 10(8) cfu/ml and incubation for 24 hours were optimal for the identification of methicillin-resistant strains. However, one single disc was not sufficient for the identification of methicillin resistance in the different staphylococcal species. The mecA-positive strains of Staphylococcus aureus and novobiocin-resistant coagulase-negative species were clearly separated from the mecA-negative strains when the 5 micrograms oxacillin disc was used, whereas the 1 microgram oxacillin disc was optimal for the identification of the mecA-positive novobiocin-sensitive coagulase-negative strains. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1109271
- author
- Petersson, A C and Miörner, Håkan LU
- organization
- publishing date
- 1995
- type
- Contribution to journal
- publication status
- published
- subject
- in
- European Journal of Clinical Microbiology & Infectious Diseases
- volume
- 14
- issue
- 3
- pages
- 206 - 211
- publisher
- Springer
- external identifiers
-
- pmid:7614961
- scopus:0028970076
- ISSN
- 1435-4373
- DOI
- 10.1007/BF02310357
- language
- English
- LU publication?
- yes
- id
- a965084a-563d-44fb-b9d2-94d7d0c443d0 (old id 1109271)
- date added to LUP
- 2016-04-01 16:08:12
- date last changed
- 2021-01-03 05:31:35
@article{a965084a-563d-44fb-b9d2-94d7d0c443d0,
abstract = {{The ability to identify methicillin-resistant staphylococci by the disc diffusion method was evaluated using discs containing oxacillin (1, 5 and 10 micrograms), methicillin (10 micrograms) and cephalexin (30 micrograms). Strains of Staphylococcus aureus (67 strains) and coagulase-negative staphylococci (72 novobiocin-sensitive and 27 novobiocin-resistant strains) were studied using two inoculum densities (10(6) cfu/ml and 10(8) cfu/ml). Inhibitory zones were recorded after 18, 24 and 42 hours of incubation. A mecA-specific application of the polymerase chain reaction was used as a reference method. The inoculum of 10(8) cfu/ml and incubation for 24 hours were optimal for the identification of methicillin-resistant strains. However, one single disc was not sufficient for the identification of methicillin resistance in the different staphylococcal species. The mecA-positive strains of Staphylococcus aureus and novobiocin-resistant coagulase-negative species were clearly separated from the mecA-negative strains when the 5 micrograms oxacillin disc was used, whereas the 1 microgram oxacillin disc was optimal for the identification of the mecA-positive novobiocin-sensitive coagulase-negative strains.}},
author = {{Petersson, A C and Miörner, Håkan}},
issn = {{1435-4373}},
language = {{eng}},
number = {{3}},
pages = {{206--211}},
publisher = {{Springer}},
series = {{European Journal of Clinical Microbiology & Infectious Diseases}},
title = {{Species-specific identification of methicillin resistance in staphylococci}},
url = {{http://dx.doi.org/10.1007/BF02310357}},
doi = {{10.1007/BF02310357}},
volume = {{14}},
year = {{1995}},
}