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Species-specific identification of methicillin resistance in staphylococci

Petersson, A C and Miörner, Håkan LU (1995) In European Journal of Clinical Microbiology & Infectious Diseases 14(3). p.206-211
Abstract
The ability to identify methicillin-resistant staphylococci by the disc diffusion method was evaluated using discs containing oxacillin (1, 5 and 10 micrograms), methicillin (10 micrograms) and cephalexin (30 micrograms). Strains of Staphylococcus aureus (67 strains) and coagulase-negative staphylococci (72 novobiocin-sensitive and 27 novobiocin-resistant strains) were studied using two inoculum densities (10(6) cfu/ml and 10(8) cfu/ml). Inhibitory zones were recorded after 18, 24 and 42 hours of incubation. A mecA-specific application of the polymerase chain reaction was used as a reference method. The inoculum of 10(8) cfu/ml and incubation for 24 hours were optimal for the identification of methicillin-resistant strains. However, one... (More)
The ability to identify methicillin-resistant staphylococci by the disc diffusion method was evaluated using discs containing oxacillin (1, 5 and 10 micrograms), methicillin (10 micrograms) and cephalexin (30 micrograms). Strains of Staphylococcus aureus (67 strains) and coagulase-negative staphylococci (72 novobiocin-sensitive and 27 novobiocin-resistant strains) were studied using two inoculum densities (10(6) cfu/ml and 10(8) cfu/ml). Inhibitory zones were recorded after 18, 24 and 42 hours of incubation. A mecA-specific application of the polymerase chain reaction was used as a reference method. The inoculum of 10(8) cfu/ml and incubation for 24 hours were optimal for the identification of methicillin-resistant strains. However, one single disc was not sufficient for the identification of methicillin resistance in the different staphylococcal species. The mecA-positive strains of Staphylococcus aureus and novobiocin-resistant coagulase-negative species were clearly separated from the mecA-negative strains when the 5 micrograms oxacillin disc was used, whereas the 1 microgram oxacillin disc was optimal for the identification of the mecA-positive novobiocin-sensitive coagulase-negative strains. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
European Journal of Clinical Microbiology & Infectious Diseases
volume
14
issue
3
pages
206 - 211
publisher
Springer
external identifiers
  • pmid:7614961
  • scopus:0028970076
ISSN
1435-4373
DOI
10.1007/BF02310357
language
English
LU publication?
yes
id
a965084a-563d-44fb-b9d2-94d7d0c443d0 (old id 1109271)
date added to LUP
2008-07-28 09:06:18
date last changed
2017-01-01 06:56:41
@article{a965084a-563d-44fb-b9d2-94d7d0c443d0,
  abstract     = {The ability to identify methicillin-resistant staphylococci by the disc diffusion method was evaluated using discs containing oxacillin (1, 5 and 10 micrograms), methicillin (10 micrograms) and cephalexin (30 micrograms). Strains of Staphylococcus aureus (67 strains) and coagulase-negative staphylococci (72 novobiocin-sensitive and 27 novobiocin-resistant strains) were studied using two inoculum densities (10(6) cfu/ml and 10(8) cfu/ml). Inhibitory zones were recorded after 18, 24 and 42 hours of incubation. A mecA-specific application of the polymerase chain reaction was used as a reference method. The inoculum of 10(8) cfu/ml and incubation for 24 hours were optimal for the identification of methicillin-resistant strains. However, one single disc was not sufficient for the identification of methicillin resistance in the different staphylococcal species. The mecA-positive strains of Staphylococcus aureus and novobiocin-resistant coagulase-negative species were clearly separated from the mecA-negative strains when the 5 micrograms oxacillin disc was used, whereas the 1 microgram oxacillin disc was optimal for the identification of the mecA-positive novobiocin-sensitive coagulase-negative strains.},
  author       = {Petersson, A C and Miörner, Håkan},
  issn         = {1435-4373},
  language     = {eng},
  number       = {3},
  pages        = {206--211},
  publisher    = {Springer},
  series       = {European Journal of Clinical Microbiology & Infectious Diseases},
  title        = {Species-specific identification of methicillin resistance in staphylococci},
  url          = {http://dx.doi.org/10.1007/BF02310357},
  volume       = {14},
  year         = {1995},
}