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Improved microscopical diagnosis of pulmonary tuberculosis in developing countries

Gebre, Negussie; Karlsson, Ulf LU ; Jönsson, Göran LU ; Macaden, Ragini; Wolde, Alemnesh; Assefa, Abraham and Miörner, Håkan LU (1995) In Transactions of the Royal Society of Tropical Medicine and Hygiene 89(2). p.191-193
Abstract
The diagnosis of pulmonary tuberculosis (TB) relies on the bacteriological examination of sputum. However, microscopy of smears made directly from sputum has a low sensitivity and there is an urgent need for improved methods. We have compared microscopy of smears made directly from sputum with microscopy after liquefaction of sputum with household bleach (NaOCl) and concentration of bacteria by centrifugation. In 3 studies performed in Ethiopia and India, the use of the NaOCl method increased the number of samples positive for acid-fast bacilli by more than 100%. The technique is appropriate for developing countries and its application would increase the efficiency of TB control programmes. As a potent disinfectant, NaOCl also has the... (More)
The diagnosis of pulmonary tuberculosis (TB) relies on the bacteriological examination of sputum. However, microscopy of smears made directly from sputum has a low sensitivity and there is an urgent need for improved methods. We have compared microscopy of smears made directly from sputum with microscopy after liquefaction of sputum with household bleach (NaOCl) and concentration of bacteria by centrifugation. In 3 studies performed in Ethiopia and India, the use of the NaOCl method increased the number of samples positive for acid-fast bacilli by more than 100%. The technique is appropriate for developing countries and its application would increase the efficiency of TB control programmes. As a potent disinfectant, NaOCl also has the advantage of lowering the risk of laboratory infection. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
tuberculosis, diagnosis by microscopy
in
Transactions of the Royal Society of Tropical Medicine and Hygiene
volume
89
issue
2
pages
191 - 193
publisher
Elsevier
external identifiers
  • pmid:7539954
  • scopus:0028905869
ISSN
1878-3503
DOI
10.1016/0035-9203(95)90491-3
language
English
LU publication?
yes
id
5f7440f7-1af4-40e9-9bbf-3bcc91ee0798 (old id 1109322)
date added to LUP
2008-07-28 09:56:23
date last changed
2017-07-30 03:43:58
@article{5f7440f7-1af4-40e9-9bbf-3bcc91ee0798,
  abstract     = {The diagnosis of pulmonary tuberculosis (TB) relies on the bacteriological examination of sputum. However, microscopy of smears made directly from sputum has a low sensitivity and there is an urgent need for improved methods. We have compared microscopy of smears made directly from sputum with microscopy after liquefaction of sputum with household bleach (NaOCl) and concentration of bacteria by centrifugation. In 3 studies performed in Ethiopia and India, the use of the NaOCl method increased the number of samples positive for acid-fast bacilli by more than 100%. The technique is appropriate for developing countries and its application would increase the efficiency of TB control programmes. As a potent disinfectant, NaOCl also has the advantage of lowering the risk of laboratory infection.},
  author       = {Gebre, Negussie and Karlsson, Ulf and Jönsson, Göran and Macaden, Ragini and Wolde, Alemnesh and Assefa, Abraham and Miörner, Håkan},
  issn         = {1878-3503},
  keyword      = {tuberculosis,diagnosis by microscopy},
  language     = {eng},
  number       = {2},
  pages        = {191--193},
  publisher    = {Elsevier},
  series       = {Transactions of the Royal Society of Tropical Medicine and Hygiene},
  title        = {Improved microscopical diagnosis of pulmonary tuberculosis in developing countries},
  url          = {http://dx.doi.org/10.1016/0035-9203(95)90491-3},
  volume       = {89},
  year         = {1995},
}