Improved microscopical diagnosis of pulmonary tuberculosis in developing countries
(1995) In Transactions of the Royal Society of Tropical Medicine and Hygiene 89(2). p.191-193- Abstract
- The diagnosis of pulmonary tuberculosis (TB) relies on the bacteriological examination of sputum. However, microscopy of smears made directly from sputum has a low sensitivity and there is an urgent need for improved methods. We have compared microscopy of smears made directly from sputum with microscopy after liquefaction of sputum with household bleach (NaOCl) and concentration of bacteria by centrifugation. In 3 studies performed in Ethiopia and India, the use of the NaOCl method increased the number of samples positive for acid-fast bacilli by more than 100%. The technique is appropriate for developing countries and its application would increase the efficiency of TB control programmes. As a potent disinfectant, NaOCl also has the... (More)
- The diagnosis of pulmonary tuberculosis (TB) relies on the bacteriological examination of sputum. However, microscopy of smears made directly from sputum has a low sensitivity and there is an urgent need for improved methods. We have compared microscopy of smears made directly from sputum with microscopy after liquefaction of sputum with household bleach (NaOCl) and concentration of bacteria by centrifugation. In 3 studies performed in Ethiopia and India, the use of the NaOCl method increased the number of samples positive for acid-fast bacilli by more than 100%. The technique is appropriate for developing countries and its application would increase the efficiency of TB control programmes. As a potent disinfectant, NaOCl also has the advantage of lowering the risk of laboratory infection. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1109322
- author
- Gebre, Negussie ; Karlsson, Ulf LU ; Jönsson, Göran LU ; Macaden, Ragini ; Wolde, Alemnesh ; Assefa, Abraham and Miörner, Håkan LU
- organization
- publishing date
- 1995
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- tuberculosis, diagnosis by microscopy
- in
- Transactions of the Royal Society of Tropical Medicine and Hygiene
- volume
- 89
- issue
- 2
- pages
- 191 - 193
- publisher
- Oxford University Press
- external identifiers
-
- pmid:7539954
- scopus:0028905869
- ISSN
- 1878-3503
- DOI
- 10.1016/0035-9203(95)90491-3
- language
- English
- LU publication?
- yes
- id
- 5f7440f7-1af4-40e9-9bbf-3bcc91ee0798 (old id 1109322)
- date added to LUP
- 2016-04-01 12:09:06
- date last changed
- 2021-01-03 03:48:06
@article{5f7440f7-1af4-40e9-9bbf-3bcc91ee0798, abstract = {{The diagnosis of pulmonary tuberculosis (TB) relies on the bacteriological examination of sputum. However, microscopy of smears made directly from sputum has a low sensitivity and there is an urgent need for improved methods. We have compared microscopy of smears made directly from sputum with microscopy after liquefaction of sputum with household bleach (NaOCl) and concentration of bacteria by centrifugation. In 3 studies performed in Ethiopia and India, the use of the NaOCl method increased the number of samples positive for acid-fast bacilli by more than 100%. The technique is appropriate for developing countries and its application would increase the efficiency of TB control programmes. As a potent disinfectant, NaOCl also has the advantage of lowering the risk of laboratory infection.}}, author = {{Gebre, Negussie and Karlsson, Ulf and Jönsson, Göran and Macaden, Ragini and Wolde, Alemnesh and Assefa, Abraham and Miörner, Håkan}}, issn = {{1878-3503}}, keywords = {{tuberculosis; diagnosis by microscopy}}, language = {{eng}}, number = {{2}}, pages = {{191--193}}, publisher = {{Oxford University Press}}, series = {{Transactions of the Royal Society of Tropical Medicine and Hygiene}}, title = {{Improved microscopical diagnosis of pulmonary tuberculosis in developing countries}}, url = {{http://dx.doi.org/10.1016/0035-9203(95)90491-3}}, doi = {{10.1016/0035-9203(95)90491-3}}, volume = {{89}}, year = {{1995}}, }