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Secretion of plasminogen activator and lysosomal enzymes from mouse skeletal muscle: effect of denervation

Vult von Steyern, Fredrik LU and Josefsson, Jan-Owe LU (1995) In Journal of Cellular Physiology 164(3). p.555-561
Abstract
Levels of hydrolytic enzymes increase in skeletal muscle after denervation and their activities in the extracellular matrix appear to be important for interaction between muscle and nerve. Using enzymatic assays for beta-glucuronidase, beta-galactosidase, and plasminogen activator, we show that secretion of these enzymes from mouse skeletal muscle increases after denervation and that drugs interfering with the secretory pathway or the reuptake of enzymes modulate this release. Thus, brefeldin A inhibited secretion of plasminogen activator activity and mannan increased secreted amounts of beta-glucuronidase, but not of beta-galactosidase, in denervated muscle. In innervated muscle, brefeldin A decreased secreted activity of plasminogen... (More)
Levels of hydrolytic enzymes increase in skeletal muscle after denervation and their activities in the extracellular matrix appear to be important for interaction between muscle and nerve. Using enzymatic assays for beta-glucuronidase, beta-galactosidase, and plasminogen activator, we show that secretion of these enzymes from mouse skeletal muscle increases after denervation and that drugs interfering with the secretory pathway or the reuptake of enzymes modulate this release. Thus, brefeldin A inhibited secretion of plasminogen activator activity and mannan increased secreted amounts of beta-glucuronidase, but not of beta-galactosidase, in denervated muscle. In innervated muscle, brefeldin A decreased secreted activity of plasminogen activator, but mannan had no effect on secretion of either beta-glucuronidase or beta-galactosidase. Furthermore, secretion of plasminogen activator was temperature dependent. These observations, together with previous studies, suggest that secretion of hydrolytic enzymes from adult skeletal muscle may be of physiological significance in nerve-muscle communication. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Cellular Physiology
volume
164
issue
3
pages
555 - 561
publisher
John Wiley & Sons
external identifiers
  • pmid:7650063
  • scopus:0029151019
ISSN
1097-4652
DOI
10.1002/jcp.1041640313
language
English
LU publication?
yes
id
6df61f98-90bc-4979-8b56-e01bb1bd7495 (old id 1109588)
date added to LUP
2008-07-29 10:27:33
date last changed
2017-01-01 04:45:01
@article{6df61f98-90bc-4979-8b56-e01bb1bd7495,
  abstract     = {Levels of hydrolytic enzymes increase in skeletal muscle after denervation and their activities in the extracellular matrix appear to be important for interaction between muscle and nerve. Using enzymatic assays for beta-glucuronidase, beta-galactosidase, and plasminogen activator, we show that secretion of these enzymes from mouse skeletal muscle increases after denervation and that drugs interfering with the secretory pathway or the reuptake of enzymes modulate this release. Thus, brefeldin A inhibited secretion of plasminogen activator activity and mannan increased secreted amounts of beta-glucuronidase, but not of beta-galactosidase, in denervated muscle. In innervated muscle, brefeldin A decreased secreted activity of plasminogen activator, but mannan had no effect on secretion of either beta-glucuronidase or beta-galactosidase. Furthermore, secretion of plasminogen activator was temperature dependent. These observations, together with previous studies, suggest that secretion of hydrolytic enzymes from adult skeletal muscle may be of physiological significance in nerve-muscle communication.},
  author       = {Vult von Steyern, Fredrik and Josefsson, Jan-Owe},
  issn         = {1097-4652},
  language     = {eng},
  number       = {3},
  pages        = {555--561},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Cellular Physiology},
  title        = {Secretion of plasminogen activator and lysosomal enzymes from mouse skeletal muscle: effect of denervation},
  url          = {http://dx.doi.org/10.1002/jcp.1041640313},
  volume       = {164},
  year         = {1995},
}