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A rapid and simple ABO genotype screening method using a novel B/O2 versus A/O2 discriminating nucleotide substitution at the ABO locus

Olsson, Martin L LU and Chester, Alan LU (1995) In Vox Sanguinis 69(3). p.242-247
Abstract
An ABO genotype screening method discriminating the common alleles A1, A2, B, O1 and O2 at the ABO locus was made possible by the discovery of a novel nucleotide substitution (G1096A) present only in B and O2 alleles. A rapid and reliable single-tube approach using multiplex PCR with four primers amplifying exons 6 and 7 of the ABO genes followed by simultaneous addition of two restriction enzymes was developed and validated in a population of 150 Swedish blood donors. This technique is the most cost-efficient and informative ABO genotyping method reported to date.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Vox Sanguinis
volume
69
issue
3
pages
242 - 247
publisher
Federation of European Neuroscience Societies and Blackwell Publishing Ltd
external identifiers
  • pmid:8578738
  • scopus:0028844274
ISSN
1423-0410
language
English
LU publication?
yes
id
fa562d06-2f99-4f4a-b30e-7b258326c973 (old id 1109592)
date added to LUP
2008-07-29 10:41:17
date last changed
2017-11-19 04:03:47
@article{fa562d06-2f99-4f4a-b30e-7b258326c973,
  abstract     = {An ABO genotype screening method discriminating the common alleles A1, A2, B, O1 and O2 at the ABO locus was made possible by the discovery of a novel nucleotide substitution (G1096A) present only in B and O2 alleles. A rapid and reliable single-tube approach using multiplex PCR with four primers amplifying exons 6 and 7 of the ABO genes followed by simultaneous addition of two restriction enzymes was developed and validated in a population of 150 Swedish blood donors. This technique is the most cost-efficient and informative ABO genotyping method reported to date.},
  author       = {Olsson, Martin L and Chester, Alan},
  issn         = {1423-0410},
  language     = {eng},
  number       = {3},
  pages        = {242--247},
  publisher    = {Federation of European Neuroscience Societies and Blackwell Publishing Ltd},
  series       = {Vox Sanguinis},
  title        = {A rapid and simple ABO genotype screening method using a novel B/O2 versus A/O2 discriminating nucleotide substitution at the ABO locus},
  volume       = {69},
  year         = {1995},
}