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Hepatitis C genotypes in Finland determined by RFLP

Pohjanpelto, Pirkko; Lappalainen, Maija; Widell, Anders LU ; Asikainen, Kari and Paunio, Mikko (1996) In Clinical and Diagnostic Virology 7(1). p.7-16
Abstract
Genotyping of hepatitis C virus (HCV) is important because of its clinical and epidemiological implications. We report here the distribution of HCV genotypes in various patient groups in Finland using a rapid and reliable HCV typing method based on restriction fragment length polymorphism (RFLP) of the amplified DNA from the 5' non-coding region of the genome. The reaction product from the primary, diagnostic PCR (nested, one tube system) was used in genotyping. From 264 Finnish sera we identified HCV genotypes 1a (14%), 1b (24%), 2b (20%). 3a (41%) and 1a + 1b (1%). Only one patient with genotype 2a was identified. From four Egyptian blood donors, types 1b and 4 were found. Genotype 3a was more often associated with i.v. drug abuse and... (More)
Genotyping of hepatitis C virus (HCV) is important because of its clinical and epidemiological implications. We report here the distribution of HCV genotypes in various patient groups in Finland using a rapid and reliable HCV typing method based on restriction fragment length polymorphism (RFLP) of the amplified DNA from the 5' non-coding region of the genome. The reaction product from the primary, diagnostic PCR (nested, one tube system) was used in genotyping. From 264 Finnish sera we identified HCV genotypes 1a (14%), 1b (24%), 2b (20%). 3a (41%) and 1a + 1b (1%). Only one patient with genotype 2a was identified. From four Egyptian blood donors, types 1b and 4 were found. Genotype 3a was more often associated with i.v. drug abuse and younger age profile (less than 30 years). (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
RFLP, PCR, Hepatitis C virus, Genotyping
in
Clinical and Diagnostic Virology
volume
7
issue
1
pages
7 - 16
publisher
Elsevier
external identifiers
  • pmid:9077432
  • scopus:0029902294
ISSN
0928-0197
DOI
10.1016/S0928-0197(96)00242-5
language
English
LU publication?
yes
id
7bc518e9-41a7-4f5b-bbf8-ef84d706b4dc (old id 1109934)
date added to LUP
2008-07-29 09:32:31
date last changed
2017-11-12 03:58:31
@article{7bc518e9-41a7-4f5b-bbf8-ef84d706b4dc,
  abstract     = {Genotyping of hepatitis C virus (HCV) is important because of its clinical and epidemiological implications. We report here the distribution of HCV genotypes in various patient groups in Finland using a rapid and reliable HCV typing method based on restriction fragment length polymorphism (RFLP) of the amplified DNA from the 5' non-coding region of the genome. The reaction product from the primary, diagnostic PCR (nested, one tube system) was used in genotyping. From 264 Finnish sera we identified HCV genotypes 1a (14%), 1b (24%), 2b (20%). 3a (41%) and 1a + 1b (1%). Only one patient with genotype 2a was identified. From four Egyptian blood donors, types 1b and 4 were found. Genotype 3a was more often associated with i.v. drug abuse and younger age profile (less than 30 years).},
  author       = {Pohjanpelto, Pirkko and Lappalainen, Maija and Widell, Anders and Asikainen, Kari and Paunio, Mikko},
  issn         = {0928-0197},
  keyword      = {RFLP,PCR,Hepatitis C virus,Genotyping},
  language     = {eng},
  number       = {1},
  pages        = {7--16},
  publisher    = {Elsevier},
  series       = {Clinical and Diagnostic Virology},
  title        = {Hepatitis C genotypes in Finland determined by RFLP},
  url          = {http://dx.doi.org/10.1016/S0928-0197(96)00242-5},
  volume       = {7},
  year         = {1996},
}