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Characterisation of recombinant isoforms of birch pollen allergen Bet v 1

Spangfort, M D ; Ipsen, H ; Sparholt, S H ; Aasmul-Olsen, S ; Osmark, Peter LU ; Poulsen, F M ; Larsen, M ; Mortz, E ; Roepstorff, P and Larsen, J N (1996) In Advances in Experimental Medicine and Biology 409. p.251-254
Abstract
Three isoforms of the major birch pollen allergen, Bet v, 1 from Betula verrucosa have been expressed as recombinant proteins in E. coli and purified. The immunochemical properties of recombinant isoforms (rBet v 1) differed on immunoblots when compared using Mabs and birch pollen allergic patients serum IgE. 2-D gel analysis showed that recombinant isoforms with different epitope structure can focus under the same protein spot after electrophoresis. The structure of conformational epitopes can be distorted by amino acid substitutions even when T-cell epitopes are not affected as judged by T-cell proliferation studies.
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author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Advances in Experimental Medicine and Biology
volume
409
pages
251 - 254
publisher
Springer
external identifiers
  • pmid:9095250
  • scopus:8244227328
ISSN
0065-2598
language
English
LU publication?
yes
id
767a1cda-e1d4-479e-ba2e-76148c470197 (old id 1110040)
date added to LUP
2016-04-01 15:59:59
date last changed
2025-04-04 14:11:53
@article{767a1cda-e1d4-479e-ba2e-76148c470197,
  abstract     = {{Three isoforms of the major birch pollen allergen, Bet v, 1 from Betula verrucosa have been expressed as recombinant proteins in E. coli and purified. The immunochemical properties of recombinant isoforms (rBet v 1) differed on immunoblots when compared using Mabs and birch pollen allergic patients serum IgE. 2-D gel analysis showed that recombinant isoforms with different epitope structure can focus under the same protein spot after electrophoresis. The structure of conformational epitopes can be distorted by amino acid substitutions even when T-cell epitopes are not affected as judged by T-cell proliferation studies.}},
  author       = {{Spangfort, M D and Ipsen, H and Sparholt, S H and Aasmul-Olsen, S and Osmark, Peter and Poulsen, F M and Larsen, M and Mortz, E and Roepstorff, P and Larsen, J N}},
  issn         = {{0065-2598}},
  language     = {{eng}},
  pages        = {{251--254}},
  publisher    = {{Springer}},
  series       = {{Advances in Experimental Medicine and Biology}},
  title        = {{Characterisation of recombinant isoforms of birch pollen allergen Bet v 1}},
  volume       = {{409}},
  year         = {{1996}},
}