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Flow cytometry analysis of malignant tumors of the head and neck--differences between two methods in the recognition of aneuploidy

Wennerberg, Johan LU ; Baldetorp, Bo LU ; Wahlberg, Peter LU and Zätterström, U K (1996) In Analytical Cellular Pathology 12(3). p.125-136
Abstract
DNA aneuploidy, which reflects changes in nuclear DNA-content, as determined cytometrically is a candidate prognostic factor in squamous cell carcinoma of the head and neck. The aim of this study was to compare two different preparation methods with respect to their use in detecting aneuploid tumor cell populations in malignant tumors of the head and neck. Fresh frozen tumor material was used. The methods compared were a multistep procedure (A) including fixation of cells and enzymatic treatment, and a one step procedure (B). Both include RNAse and the use of propidium iodide for DNA staining. Forty-seven percent of the tumors were non-diploid according to method A, and 29% according to method B, discordant findings being made in 15... (More)
DNA aneuploidy, which reflects changes in nuclear DNA-content, as determined cytometrically is a candidate prognostic factor in squamous cell carcinoma of the head and neck. The aim of this study was to compare two different preparation methods with respect to their use in detecting aneuploid tumor cell populations in malignant tumors of the head and neck. Fresh frozen tumor material was used. The methods compared were a multistep procedure (A) including fixation of cells and enzymatic treatment, and a one step procedure (B). Both include RNAse and the use of propidium iodide for DNA staining. Forty-seven percent of the tumors were non-diploid according to method A, and 29% according to method B, discordant findings being made in 15 tumors, only two of which were non-SCC. Defining a 'true non-diploid tumor' in this series as a tumor with a DNA index outside the range of diploidy detected either by method A or B, 59% (29/49) of the tumors were non-diploid. The sensitivity was 0.48 (14/29) for method B and 0.79 (23/29) for method A. The striking differences in accuracy between methods A and B emphasize the need of caution when new methods are introduced, and when results obtained with different methods are compared. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Cellular Pathology
volume
12
issue
3
pages
125 - 136
publisher
IOS Press
external identifiers
  • pmid:9025989
  • scopus:0030498341
ISSN
0921-8912
language
English
LU publication?
yes
id
81833326-b4ab-4b63-bcbc-0bc75b2e9774 (old id 1110069)
date added to LUP
2008-07-30 10:00:43
date last changed
2017-01-01 06:40:16
@article{81833326-b4ab-4b63-bcbc-0bc75b2e9774,
  abstract     = {DNA aneuploidy, which reflects changes in nuclear DNA-content, as determined cytometrically is a candidate prognostic factor in squamous cell carcinoma of the head and neck. The aim of this study was to compare two different preparation methods with respect to their use in detecting aneuploid tumor cell populations in malignant tumors of the head and neck. Fresh frozen tumor material was used. The methods compared were a multistep procedure (A) including fixation of cells and enzymatic treatment, and a one step procedure (B). Both include RNAse and the use of propidium iodide for DNA staining. Forty-seven percent of the tumors were non-diploid according to method A, and 29% according to method B, discordant findings being made in 15 tumors, only two of which were non-SCC. Defining a 'true non-diploid tumor' in this series as a tumor with a DNA index outside the range of diploidy detected either by method A or B, 59% (29/49) of the tumors were non-diploid. The sensitivity was 0.48 (14/29) for method B and 0.79 (23/29) for method A. The striking differences in accuracy between methods A and B emphasize the need of caution when new methods are introduced, and when results obtained with different methods are compared.},
  author       = {Wennerberg, Johan and Baldetorp, Bo and Wahlberg, Peter and Zätterström, U K},
  issn         = {0921-8912},
  language     = {eng},
  number       = {3},
  pages        = {125--136},
  publisher    = {IOS Press},
  series       = {Analytical Cellular Pathology},
  title        = {Flow cytometry analysis of malignant tumors of the head and neck--differences between two methods in the recognition of aneuploidy},
  volume       = {12},
  year         = {1996},
}