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Upregulation of gap junction protein connexin43 in alveolar epithelial cells of rats with radiation-induced pulmonary fibrosis

Kasper, Michael; Traub, Otto; Reimann, Thomas; Bjermer, Leif LU ; Grossmann, Heinz; Muller, Martin and Wenzel, Klaus-Wolfgang (1996) In Histochemistry and Cell Biology 106(4). p.419-424
Abstract
The degree of immunoreactive connexin43 (C x 43) in rat lung was evaluated during the development of radiation-induced pulmonary fibrosis in rat by a double immunofluorescence technique using polyclonal antisera to Cx43 and monoclonal antibodies to cytokeratins on cryostat sections. In normal rat lungs, Cx43 was detected in pneumocytes type II and I, in large blood vessel endothelia, in peribronchial smooth muscle cells, and in some peribronchial and perivascular interstitial cells. As early as 1 week after irradiation, enhanced immunoreactivity for Cx43 in the epithelial cells was detected. In severely injured lungs (about 3 months after irradiation), Cx43 was found also in the cytoplasm of type II pneumocytes. These findings were... (More)
The degree of immunoreactive connexin43 (C x 43) in rat lung was evaluated during the development of radiation-induced pulmonary fibrosis in rat by a double immunofluorescence technique using polyclonal antisera to Cx43 and monoclonal antibodies to cytokeratins on cryostat sections. In normal rat lungs, Cx43 was detected in pneumocytes type II and I, in large blood vessel endothelia, in peribronchial smooth muscle cells, and in some peribronchial and perivascular interstitial cells. As early as 1 week after irradiation, enhanced immunoreactivity for Cx43 in the epithelial cells was detected. In severely injured lungs (about 3 months after irradiation), Cx43 was found also in the cytoplasm of type II pneumocytes. These findings were confirmed by western blot data. Western blot analysis also revealed increased phosphorylation of Cx43. It remains to be investigated whether the increased content of Cx43 in irradiated rat lung may be due to an enhanced number of gap junctions between type I and II alveolar epithelial cells. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Histochemistry and Cell Biology
volume
106
issue
4
pages
419 - 424
publisher
Springer
external identifiers
  • pmid:8911970
  • scopus:0029801810
ISSN
1432-119X
DOI
10.1007/BF02473301
language
English
LU publication?
no
id
12db0226-0f07-4b7a-87a2-c31f3fd65bbe (old id 1110102)
date added to LUP
2008-07-24 11:28:44
date last changed
2017-07-30 03:45:56
@article{12db0226-0f07-4b7a-87a2-c31f3fd65bbe,
  abstract     = {The degree of immunoreactive connexin43 (C x 43) in rat lung was evaluated during the development of radiation-induced pulmonary fibrosis in rat by a double immunofluorescence technique using polyclonal antisera to Cx43 and monoclonal antibodies to cytokeratins on cryostat sections. In normal rat lungs, Cx43 was detected in pneumocytes type II and I, in large blood vessel endothelia, in peribronchial smooth muscle cells, and in some peribronchial and perivascular interstitial cells. As early as 1 week after irradiation, enhanced immunoreactivity for Cx43 in the epithelial cells was detected. In severely injured lungs (about 3 months after irradiation), Cx43 was found also in the cytoplasm of type II pneumocytes. These findings were confirmed by western blot data. Western blot analysis also revealed increased phosphorylation of Cx43. It remains to be investigated whether the increased content of Cx43 in irradiated rat lung may be due to an enhanced number of gap junctions between type I and II alveolar epithelial cells.},
  author       = {Kasper, Michael and Traub, Otto and Reimann, Thomas and Bjermer, Leif and Grossmann, Heinz and Muller, Martin and Wenzel, Klaus-Wolfgang},
  issn         = {1432-119X},
  language     = {eng},
  number       = {4},
  pages        = {419--424},
  publisher    = {Springer},
  series       = {Histochemistry and Cell Biology},
  title        = {Upregulation of gap junction protein connexin43 in alveolar epithelial cells of rats with radiation-induced pulmonary fibrosis},
  url          = {http://dx.doi.org/10.1007/BF02473301},
  volume       = {106},
  year         = {1996},
}