Induction of IL-4 by platelet-activating factor
(1996) In Clinical and Experimental Immunology 106(1). p.143-148- Abstract
- Platelet-activating factor (PAF) is a phospholipid inflammatory mediator which is synthesized by a variety of cells, including monocytes, endothelial cells, mast cells and neutrophils. PAF acts via a recently cloned PAF receptor, present on monocytes and endothelial cells, but not on non-activated lymphocytes. IL-4 is mainly produced by T lymphocytes, and belongs to the Th2 subset of T helper cells. IL-6 is mainly a monocyte/macrophage-derived cytokine with multiple proinflammatory effects. We here report that PAF induces IL-4 production, as determined by ELISPOT. Antibodies to MHC class II inhibited the IL-4 stimulatory effects of PAF. PAF also had the capacity to induce IgA production, as determined by ELISPOT, and IL-6 production in... (More)
- Platelet-activating factor (PAF) is a phospholipid inflammatory mediator which is synthesized by a variety of cells, including monocytes, endothelial cells, mast cells and neutrophils. PAF acts via a recently cloned PAF receptor, present on monocytes and endothelial cells, but not on non-activated lymphocytes. IL-4 is mainly produced by T lymphocytes, and belongs to the Th2 subset of T helper cells. IL-6 is mainly a monocyte/macrophage-derived cytokine with multiple proinflammatory effects. We here report that PAF induces IL-4 production, as determined by ELISPOT. Antibodies to MHC class II inhibited the IL-4 stimulatory effects of PAF. PAF also had the capacity to induce IgA production, as determined by ELISPOT, and IL-6 production in peripheral blood mononuclear cells (PBMC) as determined by ELISA. These PAF-mediated effects were completely inhibited by a specific PAF-receptor antagonist, WEB 2170. Taken together, our data indicate that PAF activates T lymphocytes to IL-4 production by an indirect, monocyte-dependent mechanism dependent on MHC class II. PAF also enhances antibody formation and IL-6 production from PBMC. These findings indicate that PAF activates immune-competent cells, which may be of importance in inflammatory diseases such as asthma, vasculitis and atherosclerosis. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1110659
- author
- Huang, Y H ; Schafer-Elinder, L ; Owman, Henrik LU ; Lorentzen, J C ; Ronnelid, J and Frostegard, J
- publishing date
- 1996
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Clinical and Experimental Immunology
- volume
- 106
- issue
- 1
- pages
- 143 - 148
- publisher
- British Society for Immunology
- external identifiers
-
- pmid:8870712
- scopus:0029782857
- ISSN
- 0009-9104
- DOI
- 10.1046/j.1365-2249.1996.d01-813.x
- language
- English
- LU publication?
- no
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Reconstructive Surgery (013240300), Joint and Soft Tissue Unit (013242920)
- id
- fa0a0543-8ae8-48a8-9beb-b5d6dc169662 (old id 1110659)
- date added to LUP
- 2016-04-01 12:02:57
- date last changed
- 2022-04-28 23:56:03
@article{fa0a0543-8ae8-48a8-9beb-b5d6dc169662, abstract = {{Platelet-activating factor (PAF) is a phospholipid inflammatory mediator which is synthesized by a variety of cells, including monocytes, endothelial cells, mast cells and neutrophils. PAF acts via a recently cloned PAF receptor, present on monocytes and endothelial cells, but not on non-activated lymphocytes. IL-4 is mainly produced by T lymphocytes, and belongs to the Th2 subset of T helper cells. IL-6 is mainly a monocyte/macrophage-derived cytokine with multiple proinflammatory effects. We here report that PAF induces IL-4 production, as determined by ELISPOT. Antibodies to MHC class II inhibited the IL-4 stimulatory effects of PAF. PAF also had the capacity to induce IgA production, as determined by ELISPOT, and IL-6 production in peripheral blood mononuclear cells (PBMC) as determined by ELISA. These PAF-mediated effects were completely inhibited by a specific PAF-receptor antagonist, WEB 2170. Taken together, our data indicate that PAF activates T lymphocytes to IL-4 production by an indirect, monocyte-dependent mechanism dependent on MHC class II. PAF also enhances antibody formation and IL-6 production from PBMC. These findings indicate that PAF activates immune-competent cells, which may be of importance in inflammatory diseases such as asthma, vasculitis and atherosclerosis.}}, author = {{Huang, Y H and Schafer-Elinder, L and Owman, Henrik and Lorentzen, J C and Ronnelid, J and Frostegard, J}}, issn = {{0009-9104}}, language = {{eng}}, number = {{1}}, pages = {{143--148}}, publisher = {{British Society for Immunology}}, series = {{Clinical and Experimental Immunology}}, title = {{Induction of IL-4 by platelet-activating factor}}, url = {{http://dx.doi.org/10.1046/j.1365-2249.1996.d01-813.x}}, doi = {{10.1046/j.1365-2249.1996.d01-813.x}}, volume = {{106}}, year = {{1996}}, }