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Induction of IL-4 by platelet-activating factor

Huang, Y H ; Schafer-Elinder, L ; Owman, Henrik LU ; Lorentzen, J C ; Ronnelid, J and Frostegard, J (1996) In Clinical and Experimental Immunology 106(1). p.143-148
Abstract
Platelet-activating factor (PAF) is a phospholipid inflammatory mediator which is synthesized by a variety of cells, including monocytes, endothelial cells, mast cells and neutrophils. PAF acts via a recently cloned PAF receptor, present on monocytes and endothelial cells, but not on non-activated lymphocytes. IL-4 is mainly produced by T lymphocytes, and belongs to the Th2 subset of T helper cells. IL-6 is mainly a monocyte/macrophage-derived cytokine with multiple proinflammatory effects. We here report that PAF induces IL-4 production, as determined by ELISPOT. Antibodies to MHC class II inhibited the IL-4 stimulatory effects of PAF. PAF also had the capacity to induce IgA production, as determined by ELISPOT, and IL-6 production in... (More)
Platelet-activating factor (PAF) is a phospholipid inflammatory mediator which is synthesized by a variety of cells, including monocytes, endothelial cells, mast cells and neutrophils. PAF acts via a recently cloned PAF receptor, present on monocytes and endothelial cells, but not on non-activated lymphocytes. IL-4 is mainly produced by T lymphocytes, and belongs to the Th2 subset of T helper cells. IL-6 is mainly a monocyte/macrophage-derived cytokine with multiple proinflammatory effects. We here report that PAF induces IL-4 production, as determined by ELISPOT. Antibodies to MHC class II inhibited the IL-4 stimulatory effects of PAF. PAF also had the capacity to induce IgA production, as determined by ELISPOT, and IL-6 production in peripheral blood mononuclear cells (PBMC) as determined by ELISA. These PAF-mediated effects were completely inhibited by a specific PAF-receptor antagonist, WEB 2170. Taken together, our data indicate that PAF activates T lymphocytes to IL-4 production by an indirect, monocyte-dependent mechanism dependent on MHC class II. PAF also enhances antibody formation and IL-6 production from PBMC. These findings indicate that PAF activates immune-competent cells, which may be of importance in inflammatory diseases such as asthma, vasculitis and atherosclerosis. (Less)
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author
; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
in
Clinical and Experimental Immunology
volume
106
issue
1
pages
143 - 148
publisher
British Society for Immunology
external identifiers
  • pmid:8870712
  • scopus:0029782857
ISSN
0009-9104
DOI
10.1046/j.1365-2249.1996.d01-813.x
language
English
LU publication?
no
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Reconstructive Surgery (013240300), Joint and Soft Tissue Unit (013242920)
id
fa0a0543-8ae8-48a8-9beb-b5d6dc169662 (old id 1110659)
date added to LUP
2016-04-01 12:02:57
date last changed
2022-04-28 23:56:03
@article{fa0a0543-8ae8-48a8-9beb-b5d6dc169662,
  abstract     = {{Platelet-activating factor (PAF) is a phospholipid inflammatory mediator which is synthesized by a variety of cells, including monocytes, endothelial cells, mast cells and neutrophils. PAF acts via a recently cloned PAF receptor, present on monocytes and endothelial cells, but not on non-activated lymphocytes. IL-4 is mainly produced by T lymphocytes, and belongs to the Th2 subset of T helper cells. IL-6 is mainly a monocyte/macrophage-derived cytokine with multiple proinflammatory effects. We here report that PAF induces IL-4 production, as determined by ELISPOT. Antibodies to MHC class II inhibited the IL-4 stimulatory effects of PAF. PAF also had the capacity to induce IgA production, as determined by ELISPOT, and IL-6 production in peripheral blood mononuclear cells (PBMC) as determined by ELISA. These PAF-mediated effects were completely inhibited by a specific PAF-receptor antagonist, WEB 2170. Taken together, our data indicate that PAF activates T lymphocytes to IL-4 production by an indirect, monocyte-dependent mechanism dependent on MHC class II. PAF also enhances antibody formation and IL-6 production from PBMC. These findings indicate that PAF activates immune-competent cells, which may be of importance in inflammatory diseases such as asthma, vasculitis and atherosclerosis.}},
  author       = {{Huang, Y H and Schafer-Elinder, L and Owman, Henrik and Lorentzen, J C and Ronnelid, J and Frostegard, J}},
  issn         = {{0009-9104}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{143--148}},
  publisher    = {{British Society for Immunology}},
  series       = {{Clinical and Experimental Immunology}},
  title        = {{Induction of IL-4 by platelet-activating factor}},
  url          = {{http://dx.doi.org/10.1046/j.1365-2249.1996.d01-813.x}},
  doi          = {{10.1046/j.1365-2249.1996.d01-813.x}},
  volume       = {{106}},
  year         = {{1996}},
}