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Early attachment of platelets, leukocytes, and fibrinogen in endothelial cell seeded Dacron grafts

Jensen, Norman; Lindblad, Bengt LU ; Ljungberg, June LU ; Leide, Sigrid and Bergqvist, David (1996) In Annals of Vascular Surgery 10(6). p.530-536
Abstract
Endothelial cell seeding has been advocated as a method for reducing the thrombogenicity of prosthetic grafts. Principally two different techniques for endothelial cell seeding can be used: immediate seeding of grafts followed by implantation or initial growth and establishment of an endothelial cell-covered surface before subsequent late implantation. This study was designed to determine whether the immediate seeding technique altered thrombogenicity directly after graft implantation. Carotid arteries from 19 sheep were replaced with Dacron interposition grafts; one side was seeded with endothelial cells and the other side was left unseeded. The dynamics of thrombus formation involving radiolabeled platelets, leukocytes, and fibrinogen... (More)
Endothelial cell seeding has been advocated as a method for reducing the thrombogenicity of prosthetic grafts. Principally two different techniques for endothelial cell seeding can be used: immediate seeding of grafts followed by implantation or initial growth and establishment of an endothelial cell-covered surface before subsequent late implantation. This study was designed to determine whether the immediate seeding technique altered thrombogenicity directly after graft implantation. Carotid arteries from 19 sheep were replaced with Dacron interposition grafts; one side was seeded with endothelial cells and the other side was left unseeded. The dynamics of thrombus formation involving radiolabeled platelets, leukocytes, and fibrinogen were studied for 4 hours with flow reduced to 35 ml/min. No difference in platelet uptake (approximately 6-fold increase compared to baseline values) was found between endothelial cell seeded and unseeded grafts. Likewise, there were no differences in leukocyte uptake (approximately 4-fold increase) or fibrinogen uptake (approximately 10- to 15-fold increase) between the two groups. No differences were demonstrated with regard to patency or thrombus weight. In this experimental investigation we were unable to verify any change in the uptake of platelets, white blood cells, or fibrinogen between endothelial cell seeded and unseeded Dacron grafts during the first 4 hours after graft placement. Immediate seeding does not affect the initial thrombogenicity of grafts. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Annals of Vascular Surgery
volume
10
issue
6
pages
530 - 536
publisher
Springer
external identifiers
  • pmid:8989969
  • scopus:0030468711
ISSN
1615-5947
DOI
10.1007/BF02000441
language
English
LU publication?
yes
id
f7ba060e-3ad0-4991-9c36-30623a49f1d0 (old id 1110797)
date added to LUP
2008-07-24 09:08:44
date last changed
2017-01-01 05:03:58
@article{f7ba060e-3ad0-4991-9c36-30623a49f1d0,
  abstract     = {Endothelial cell seeding has been advocated as a method for reducing the thrombogenicity of prosthetic grafts. Principally two different techniques for endothelial cell seeding can be used: immediate seeding of grafts followed by implantation or initial growth and establishment of an endothelial cell-covered surface before subsequent late implantation. This study was designed to determine whether the immediate seeding technique altered thrombogenicity directly after graft implantation. Carotid arteries from 19 sheep were replaced with Dacron interposition grafts; one side was seeded with endothelial cells and the other side was left unseeded. The dynamics of thrombus formation involving radiolabeled platelets, leukocytes, and fibrinogen were studied for 4 hours with flow reduced to 35 ml/min. No difference in platelet uptake (approximately 6-fold increase compared to baseline values) was found between endothelial cell seeded and unseeded grafts. Likewise, there were no differences in leukocyte uptake (approximately 4-fold increase) or fibrinogen uptake (approximately 10- to 15-fold increase) between the two groups. No differences were demonstrated with regard to patency or thrombus weight. In this experimental investigation we were unable to verify any change in the uptake of platelets, white blood cells, or fibrinogen between endothelial cell seeded and unseeded Dacron grafts during the first 4 hours after graft placement. Immediate seeding does not affect the initial thrombogenicity of grafts.},
  author       = {Jensen, Norman and Lindblad, Bengt and Ljungberg, June and Leide, Sigrid and Bergqvist, David},
  issn         = {1615-5947},
  language     = {eng},
  number       = {6},
  pages        = {530--536},
  publisher    = {Springer},
  series       = {Annals of Vascular Surgery},
  title        = {Early attachment of platelets, leukocytes, and fibrinogen in endothelial cell seeded Dacron grafts},
  url          = {http://dx.doi.org/10.1007/BF02000441},
  volume       = {10},
  year         = {1996},
}