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Carbohydrate-deficient transferrin measured by high-performance liquid chromatography and CDTect immunoassay

Simonsson, Per LU ; Lindberg, S and Alling, Christer LU (1996) In Alcohol and Alcoholism 31(4). p.397-402
Abstract
Carbohydrate-deficient transferrin (CDT) was measured in three populations using anion-exchange chromatography followed by radioimmunoassay (CDTectTM) and a new high-performance liquid chromatography (HPLC) method. The correlation between the methods in 50 consecutive clinical samples was good (r = 0.87). However, in a set of 49 samples with CDT concentrations close to the reference value of the methods, the correlation was low (r = 0.51). In addition, among controls, no correlation between the methods was found (r = 0.10). Abnormal isoforms of transferrin were noted in 5% of the clinical samples. HPLC determination of CDT offers the advantage of identifying these isoforms of transferrin and thus reducing analytical pitfalls associated... (More)
Carbohydrate-deficient transferrin (CDT) was measured in three populations using anion-exchange chromatography followed by radioimmunoassay (CDTectTM) and a new high-performance liquid chromatography (HPLC) method. The correlation between the methods in 50 consecutive clinical samples was good (r = 0.87). However, in a set of 49 samples with CDT concentrations close to the reference value of the methods, the correlation was low (r = 0.51). In addition, among controls, no correlation between the methods was found (r = 0.10). Abnormal isoforms of transferrin were noted in 5% of the clinical samples. HPLC determination of CDT offers the advantage of identifying these isoforms of transferrin and thus reducing analytical pitfalls associated with the CDTectTM method. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Alcohol and Alcoholism
volume
31
issue
4
pages
397 - 402
publisher
Oxford University Press
external identifiers
  • pmid:8879289
  • scopus:0029743406
ISSN
1464-3502
language
English
LU publication?
yes
id
ea2930c2-105c-476a-825a-4b9db124d83d (old id 1110826)
alternative location
http://alcalc.oxfordjournals.org/cgi/reprint/31/4/397.pdf
date added to LUP
2008-07-29 11:15:30
date last changed
2017-01-01 05:19:26
@article{ea2930c2-105c-476a-825a-4b9db124d83d,
  abstract     = {Carbohydrate-deficient transferrin (CDT) was measured in three populations using anion-exchange chromatography followed by radioimmunoassay (CDTectTM) and a new high-performance liquid chromatography (HPLC) method. The correlation between the methods in 50 consecutive clinical samples was good (r = 0.87). However, in a set of 49 samples with CDT concentrations close to the reference value of the methods, the correlation was low (r = 0.51). In addition, among controls, no correlation between the methods was found (r = 0.10). Abnormal isoforms of transferrin were noted in 5% of the clinical samples. HPLC determination of CDT offers the advantage of identifying these isoforms of transferrin and thus reducing analytical pitfalls associated with the CDTectTM method.},
  author       = {Simonsson, Per and Lindberg, S and Alling, Christer},
  issn         = {1464-3502},
  language     = {eng},
  number       = {4},
  pages        = {397--402},
  publisher    = {Oxford University Press},
  series       = {Alcohol and Alcoholism},
  title        = {Carbohydrate-deficient transferrin measured by high-performance liquid chromatography and CDTect immunoassay},
  volume       = {31},
  year         = {1996},
}