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Dystroglycan is required for polarizing the epithelial cells and the oocyte in Drosophila.

Deng, Wu-Min; Schneider, Martina LU ; Frock, Richard; Castillejo-Lopez, Casimiro LU ; Gaman, Emily Anne; Baumgartner, Stefan LU and Ruohola-Baker, Hannele (2003) In Development 130(1). p.173-184
Abstract
The transmembrane protein Dystroglycan is a central element of the dystrophin-associated glycoprotein complex, which is involved in the pathogenesis of many forms of muscular dystrophy. Dystroglycan is a receptor for multiple extracellular matrix (ECM) molecules such as Laminin, agrin and perlecan, and plays a role in linking the ECM to the actin cytoskeleton; however, how these interactions are regulated and their basic cellular functions are poorly understood. Using mosaic analysis and RNAi in the model organism Drosophila melanogaster, we show that Dystroglycan is required cell-autonomously for cellular polarity in two different cell types, the epithelial cells (apicobasal polarity) and the oocyte (anteroposterior polarity). Loss of... (More)
The transmembrane protein Dystroglycan is a central element of the dystrophin-associated glycoprotein complex, which is involved in the pathogenesis of many forms of muscular dystrophy. Dystroglycan is a receptor for multiple extracellular matrix (ECM) molecules such as Laminin, agrin and perlecan, and plays a role in linking the ECM to the actin cytoskeleton; however, how these interactions are regulated and their basic cellular functions are poorly understood. Using mosaic analysis and RNAi in the model organism Drosophila melanogaster, we show that Dystroglycan is required cell-autonomously for cellular polarity in two different cell types, the epithelial cells (apicobasal polarity) and the oocyte (anteroposterior polarity). Loss of Dystroglycan function in follicle and disc epithelia results in expansion of apical markers to the basal side of cells and overexpression results in a reduced apical localization of these same markers. In Dystroglycan germline clones early oocyte polarity markers fail to be localized to the posterior, and oocyte cortical F-actin organization is abnormal. Dystroglycan is also required non-cell-autonomously to organize the planar polarity of basal actin in follicle cells, possibly by organizing the Laminin ECM. These data suggest that the primary function of Dystroglycan in oogenesis is to organize cellular polarity; and this study sets the stage for analyzing the Dystroglycan complex by using the power of Drosophila molecular genetics. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Development
volume
130
issue
1
pages
173 - 184
publisher
Society for International Development
external identifiers
  • pmid:12441301
  • wos:000180537900016
  • scopus:0037235163
ISSN
1477-9129
DOI
10.1242/10.1242/dev.00199
language
English
LU publication?
yes
id
5f173e96-c6fe-4b5f-8f37-b070076bc387 (old id 111111)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12441301&dopt=Abstract
date added to LUP
2007-07-11 12:52:13
date last changed
2018-01-07 06:06:20
@article{5f173e96-c6fe-4b5f-8f37-b070076bc387,
  abstract     = {The transmembrane protein Dystroglycan is a central element of the dystrophin-associated glycoprotein complex, which is involved in the pathogenesis of many forms of muscular dystrophy. Dystroglycan is a receptor for multiple extracellular matrix (ECM) molecules such as Laminin, agrin and perlecan, and plays a role in linking the ECM to the actin cytoskeleton; however, how these interactions are regulated and their basic cellular functions are poorly understood. Using mosaic analysis and RNAi in the model organism Drosophila melanogaster, we show that Dystroglycan is required cell-autonomously for cellular polarity in two different cell types, the epithelial cells (apicobasal polarity) and the oocyte (anteroposterior polarity). Loss of Dystroglycan function in follicle and disc epithelia results in expansion of apical markers to the basal side of cells and overexpression results in a reduced apical localization of these same markers. In Dystroglycan germline clones early oocyte polarity markers fail to be localized to the posterior, and oocyte cortical F-actin organization is abnormal. Dystroglycan is also required non-cell-autonomously to organize the planar polarity of basal actin in follicle cells, possibly by organizing the Laminin ECM. These data suggest that the primary function of Dystroglycan in oogenesis is to organize cellular polarity; and this study sets the stage for analyzing the Dystroglycan complex by using the power of Drosophila molecular genetics.},
  author       = {Deng, Wu-Min and Schneider, Martina and Frock, Richard and Castillejo-Lopez, Casimiro and Gaman, Emily Anne and Baumgartner, Stefan and Ruohola-Baker, Hannele},
  issn         = {1477-9129},
  language     = {eng},
  number       = {1},
  pages        = {173--184},
  publisher    = {Society for International Development},
  series       = {Development},
  title        = {Dystroglycan is required for polarizing the epithelial cells and the oocyte in Drosophila.},
  url          = {http://dx.doi.org/10.1242/10.1242/dev.00199},
  volume       = {130},
  year         = {2003},
}