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Hepatitis C virus genotypes in different regions of the former Soviet Union (Russia, Belarus, Moldova, and Uzbekistan)

Viazov, S; Kuzin, S; Paladi, N; Tchernovetsky, M; Isaeva, E; Mazhul, L; Vasychova, F; Widell, Anders LU and Roggendorf, M (1997) In Journal of Medical Virology 53(1). p.36-40
Abstract
The prevalence of HCV genotypes in four republics of the former Soviet Union (Russia, Belarus, Moldova, and Uzbekistan) was investigated. Overall, 197 HCV isolates from 66 blood donors and 131 patients with chronic hepatitis were typed. Viral sequences from sera of infected subjects were amplified by nested RT-PCR using primers from the core region and typed by one or two techniques: (1) DNA enzyme immunoassay (DEIA) and (2) PCR with a set of type-specific primers. Only three major HCV genotypes were identified in this study population. HCV 1b was found to be the predominant virus type both among blood donors and chronic hepatitis patients, followed by 3a, 2a, and 1a (chronic hepatitis patients: 1b-82%; 3a-10%; 2a-4%, 1a-5% and 2c-1%;... (More)
The prevalence of HCV genotypes in four republics of the former Soviet Union (Russia, Belarus, Moldova, and Uzbekistan) was investigated. Overall, 197 HCV isolates from 66 blood donors and 131 patients with chronic hepatitis were typed. Viral sequences from sera of infected subjects were amplified by nested RT-PCR using primers from the core region and typed by one or two techniques: (1) DNA enzyme immunoassay (DEIA) and (2) PCR with a set of type-specific primers. Only three major HCV genotypes were identified in this study population. HCV 1b was found to be the predominant virus type both among blood donors and chronic hepatitis patients, followed by 3a, 2a, and 1a (chronic hepatitis patients: 1b-82%; 3a-10%; 2a-4%, 1a-5% and 2c-1%; blood donors: 1b-77%; 3a-17%; and 2a-6%). No significant difference in genotype distribution was observed between different countries or between blood donors and chronic hepatitis patients within the same country. Results of the genotyping procedures were confirmed by direct sequencing of 216 nt PCR fragments corresponding to part of HCV core gene. Phylogenetic analysis of HCV 1b sequences from this study and from the Genbank demonstrated that the sequences from the former Soviet Union do not form evolutionary lineage(s) different from those of strains of the same subtype circulating in other geographical regions. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Medical Virology
volume
53
issue
1
pages
36 - 40
publisher
John Wiley & Sons
external identifiers
  • pmid:9298730
  • scopus:0030827758
ISSN
1096-9071
DOI
10.1002/(SICI)1096-9071(199709)53:1<36::AID-JMV7>3.0.CO;2-R
language
English
LU publication?
yes
id
f50ad613-7491-4ef8-b47c-90b0e5e84ec8 (old id 1111269)
date added to LUP
2008-07-17 10:33:56
date last changed
2017-07-09 03:40:26
@article{f50ad613-7491-4ef8-b47c-90b0e5e84ec8,
  abstract     = {The prevalence of HCV genotypes in four republics of the former Soviet Union (Russia, Belarus, Moldova, and Uzbekistan) was investigated. Overall, 197 HCV isolates from 66 blood donors and 131 patients with chronic hepatitis were typed. Viral sequences from sera of infected subjects were amplified by nested RT-PCR using primers from the core region and typed by one or two techniques: (1) DNA enzyme immunoassay (DEIA) and (2) PCR with a set of type-specific primers. Only three major HCV genotypes were identified in this study population. HCV 1b was found to be the predominant virus type both among blood donors and chronic hepatitis patients, followed by 3a, 2a, and 1a (chronic hepatitis patients: 1b-82%; 3a-10%; 2a-4%, 1a-5% and 2c-1%; blood donors: 1b-77%; 3a-17%; and 2a-6%). No significant difference in genotype distribution was observed between different countries or between blood donors and chronic hepatitis patients within the same country. Results of the genotyping procedures were confirmed by direct sequencing of 216 nt PCR fragments corresponding to part of HCV core gene. Phylogenetic analysis of HCV 1b sequences from this study and from the Genbank demonstrated that the sequences from the former Soviet Union do not form evolutionary lineage(s) different from those of strains of the same subtype circulating in other geographical regions.},
  author       = {Viazov, S and Kuzin, S and Paladi, N and Tchernovetsky, M and Isaeva, E and Mazhul, L and Vasychova, F and Widell, Anders and Roggendorf, M},
  issn         = {1096-9071},
  language     = {eng},
  number       = {1},
  pages        = {36--40},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Medical Virology},
  title        = {Hepatitis C virus genotypes in different regions of the former Soviet Union (Russia, Belarus, Moldova, and Uzbekistan)},
  url          = {http://dx.doi.org/10.1002/(SICI)1096-9071(199709)53:1<36::AID-JMV7>3.0.CO;2-R},
  volume       = {53},
  year         = {1997},
}