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Activated cell cycle checkpoints in epirubicin-treated breast cancer cells studied by BrdUrd-flow cytometry

Hedenfalk, Ingrid LU ; Baldetorp, Bo LU ; Borg, Åke LU and Oredsson, Stina LU (1997) In Cytometry 29(4). p.321-327
Abstract
Genetic alterations, such as p53 mutations, may affect a tumour's response to chemotherapy. We have treated two human breast cancer cell lines that differ in p53 status with epirubicin in order to study if there are differences in cell cycle kinetic response. MCF-7 cells express wild-type p53, while SK-BR-3 cells express only a mutated form of p53. The transition of cells from one cell cycle stage to another was studied by a bromodeoxyuridine (BrdUrd)-flow cytometry (FCM) method. MCF-7 cells showed a block in the G1 phase after treatment with 50 nM epirubicin for 24 hours, in agreement with the actions of p53 at the G1 checkpoint. SK-BR-3 cells, on the other hand, progressed through the G1 checkpoint and were blocked in late S and G2... (More)
Genetic alterations, such as p53 mutations, may affect a tumour's response to chemotherapy. We have treated two human breast cancer cell lines that differ in p53 status with epirubicin in order to study if there are differences in cell cycle kinetic response. MCF-7 cells express wild-type p53, while SK-BR-3 cells express only a mutated form of p53. The transition of cells from one cell cycle stage to another was studied by a bromodeoxyuridine (BrdUrd)-flow cytometry (FCM) method. MCF-7 cells showed a block in the G1 phase after treatment with 50 nM epirubicin for 24 hours, in agreement with the actions of p53 at the G1 checkpoint. SK-BR-3 cells, on the other hand, progressed through the G1 checkpoint and were blocked in late S and G2 phases, presumably due to the activation of a later checkpoint. In addition, studies of the mRNA levels of p53 and its effector gene p21 revealed that although both cell lines expressed p53 mRNA, a marked difference in the mRNA levels of p21 was seen. A dramatic increase in the level of p21 mRNA was seen in epirubicin-treated MCF-7 cells, while no such increase was seen in SK-BR-3 cells. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
breast cancer, epirubicin, p21, RNA, p53, bromodeoxyuridine, flow cytometry, cell cycle kinetics
in
Cytometry
volume
29
issue
4
pages
321 - 327
publisher
John Wiley & Sons
external identifiers
  • pmid:9415415
  • scopus:0031450362
ISSN
0196-4763
DOI
10.1002/(SICI)1097-0320(19971201)29:4<321::AID-CYTO9>3.0.CO;2-C
language
English
LU publication?
yes
id
eaee408b-529f-4319-87bd-219f8eeb2730 (old id 1111386)
date added to LUP
2008-07-17 12:01:51
date last changed
2017-01-01 07:09:48
@article{eaee408b-529f-4319-87bd-219f8eeb2730,
  abstract     = {Genetic alterations, such as p53 mutations, may affect a tumour's response to chemotherapy. We have treated two human breast cancer cell lines that differ in p53 status with epirubicin in order to study if there are differences in cell cycle kinetic response. MCF-7 cells express wild-type p53, while SK-BR-3 cells express only a mutated form of p53. The transition of cells from one cell cycle stage to another was studied by a bromodeoxyuridine (BrdUrd)-flow cytometry (FCM) method. MCF-7 cells showed a block in the G1 phase after treatment with 50 nM epirubicin for 24 hours, in agreement with the actions of p53 at the G1 checkpoint. SK-BR-3 cells, on the other hand, progressed through the G1 checkpoint and were blocked in late S and G2 phases, presumably due to the activation of a later checkpoint. In addition, studies of the mRNA levels of p53 and its effector gene p21 revealed that although both cell lines expressed p53 mRNA, a marked difference in the mRNA levels of p21 was seen. A dramatic increase in the level of p21 mRNA was seen in epirubicin-treated MCF-7 cells, while no such increase was seen in SK-BR-3 cells.},
  author       = {Hedenfalk, Ingrid and Baldetorp, Bo and Borg, Åke and Oredsson, Stina},
  issn         = {0196-4763},
  keyword      = {breast cancer,epirubicin,p21,RNA,p53,bromodeoxyuridine,flow cytometry,cell cycle kinetics},
  language     = {eng},
  number       = {4},
  pages        = {321--327},
  publisher    = {John Wiley & Sons},
  series       = {Cytometry},
  title        = {Activated cell cycle checkpoints in epirubicin-treated breast cancer cells studied by BrdUrd-flow cytometry},
  url          = {http://dx.doi.org/10.1002/(SICI)1097-0320(19971201)29:4<321::AID-CYTO9>3.0.CO;2-C},
  volume       = {29},
  year         = {1997},
}