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Intra- and inter-laboratory reproducibility of estrogen and progesterone receptor enzyme immunoassay in breast cancer cytosol samples--a Swedish multicenter study. Swedish Society of Cancer Study Group

Fernö, Mårten LU ; Bendahl, Pär-Ola LU ; Brisfors, Ann; Byman, Karin; Ekeberg, Maud; Ferraud, Lilianne; Grankvist, Kjell; Hjalmers, Bertil; Nilsson, Anita and Sellberg, Gunilla LU , et al. (1997) In Acta Oncologica 36(8). p.793-798
Abstract
Estrogen and progesterone receptor analysis results were compared within and between six laboratories in Sweden using frozen breast cancer cytosol samples, and the same technique (enzyme immunoassay, Abbott Laboratories). The concordance in receptor status (positive vs. negative) was excellent (98.4% (571/580)). The discordant results were attributable to values near cut-off (n = 4) or outliers (n = 5), the latter probably being due to analytical errors. One laboratory reported significantly higher ER concentrations than the others; thus caution should be observed when comparing absolute values from different centers. For PgR there were similar differences between the laboratories. However, the intra- and inter-laboratory differences were... (More)
Estrogen and progesterone receptor analysis results were compared within and between six laboratories in Sweden using frozen breast cancer cytosol samples, and the same technique (enzyme immunoassay, Abbott Laboratories). The concordance in receptor status (positive vs. negative) was excellent (98.4% (571/580)). The discordant results were attributable to values near cut-off (n = 4) or outliers (n = 5), the latter probably being due to analytical errors. One laboratory reported significantly higher ER concentrations than the others; thus caution should be observed when comparing absolute values from different centers. For PgR there were similar differences between the laboratories. However, the intra- and inter-laboratory differences were small compared with the overall variability in ER and PgR content between different samples in a large database. The range of the median intra-laboratory coefficient of variation was 11-23% for ER and 12-19% for PgR, indicating that there is room for improvement in the quality of assay performance. (Less)
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publication status
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Acta Oncologica
volume
36
issue
8
pages
793 - 798
publisher
Taylor & Francis
external identifiers
  • pmid:9482684
ISSN
1651-226X
DOI
10.3109/02841869709001359
language
English
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yes
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b81a49f2-3a8d-4a3b-abe0-2a8492e7bf13 (old id 1111546)
date added to LUP
2008-07-17 16:01:25
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2016-04-16 05:28:24
@article{b81a49f2-3a8d-4a3b-abe0-2a8492e7bf13,
  abstract     = {Estrogen and progesterone receptor analysis results were compared within and between six laboratories in Sweden using frozen breast cancer cytosol samples, and the same technique (enzyme immunoassay, Abbott Laboratories). The concordance in receptor status (positive vs. negative) was excellent (98.4% (571/580)). The discordant results were attributable to values near cut-off (n = 4) or outliers (n = 5), the latter probably being due to analytical errors. One laboratory reported significantly higher ER concentrations than the others; thus caution should be observed when comparing absolute values from different centers. For PgR there were similar differences between the laboratories. However, the intra- and inter-laboratory differences were small compared with the overall variability in ER and PgR content between different samples in a large database. The range of the median intra-laboratory coefficient of variation was 11-23% for ER and 12-19% for PgR, indicating that there is room for improvement in the quality of assay performance.},
  author       = {Fernö, Mårten and Bendahl, Pär-Ola and Brisfors, Ann and Byman, Karin and Ekeberg, Maud and Ferraud, Lilianne and Grankvist, Kjell and Hjalmers, Bertil and Nilsson, Anita and Sellberg, Gunilla and Skoog, Lambert and Stal, Olle and Wingmo, Ingalill},
  issn         = {1651-226X},
  language     = {eng},
  number       = {8},
  pages        = {793--798},
  publisher    = {Taylor & Francis},
  series       = {Acta Oncologica},
  title        = {Intra- and inter-laboratory reproducibility of estrogen and progesterone receptor enzyme immunoassay in breast cancer cytosol samples--a Swedish multicenter study. Swedish Society of Cancer Study Group},
  url          = {http://dx.doi.org/10.3109/02841869709001359},
  volume       = {36},
  year         = {1997},
}