Advanced

Analysis of 5-hydroxy-N-methyl-2-pyrrolidone and 2-hydroxy-N-methylsuccinimide in plasma

Jonsson, B A G and Åkesson, Bengt LU (1997) In Chromatographia 46(3-4). p.141-144
Abstract
A method for the determination of 5-hydroxy-N-methyl-2-pyrrolidone (5-HNMP) and 2-hydroxy-N-methylsuccinimide (2-HMSI) in plasma was developed. 5-HNMP and 2-HMSI are metabolites to the widely used organic solvent N-methyl-2pyrrolidone (NMP). The 5-HNMP and 2-HMSI were purified from plasma by C8 solid phase extraction, derivatised by bistrimethylsilyl trifluoroacetamid, and analysed by gas chromatography with mass spectrometric detection. For 5-HNMP, the precision was 2–7 % (120 and 780 ng mL−1) and the detection limit was 6 ng mL−1 (m/z 98). For 2-HMSI, the precision was 2–9 % (160 and 1000 ng mL−1) and the detection limit was 4 ng mL−1 (m/z 144). The method is applicable for analysis of plasma samples from workers exposed to NMP.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Gas chromatography-mass spectrometry, Biological monitoring, 5-hydroxy-N-methyl-2-pyrrolidone, 2-hydroxy-N-methylsuccinimide, Plasma samples
in
Chromatographia
volume
46
issue
3-4
pages
141 - 144
publisher
Vieweg Verlag
external identifiers
  • scopus:0030810963
ISSN
0009-5893
DOI
10.1007/BF02495324
language
English
LU publication?
yes
id
05abd1f8-9cb5-40cc-b54e-f4330581eb87 (old id 1111900)
date added to LUP
2008-07-18 15:54:04
date last changed
2017-01-01 07:28:37
@article{05abd1f8-9cb5-40cc-b54e-f4330581eb87,
  abstract     = {A method for the determination of 5-hydroxy-N-methyl-2-pyrrolidone (5-HNMP) and 2-hydroxy-N-methylsuccinimide (2-HMSI) in plasma was developed. 5-HNMP and 2-HMSI are metabolites to the widely used organic solvent N-methyl-2pyrrolidone (NMP). The 5-HNMP and 2-HMSI were purified from plasma by C8 solid phase extraction, derivatised by bistrimethylsilyl trifluoroacetamid, and analysed by gas chromatography with mass spectrometric detection. For 5-HNMP, the precision was 2–7 % (120 and 780 ng mL−1) and the detection limit was 6 ng mL−1 (m/z 98). For 2-HMSI, the precision was 2–9 % (160 and 1000 ng mL−1) and the detection limit was 4 ng mL−1 (m/z 144). The method is applicable for analysis of plasma samples from workers exposed to NMP.},
  author       = {Jonsson, B A G and Åkesson, Bengt},
  issn         = {0009-5893},
  keyword      = {Gas chromatography-mass spectrometry,Biological monitoring,5-hydroxy-N-methyl-2-pyrrolidone,2-hydroxy-N-methylsuccinimide,Plasma samples},
  language     = {eng},
  number       = {3-4},
  pages        = {141--144},
  publisher    = {Vieweg Verlag},
  series       = {Chromatographia},
  title        = {Analysis of 5-hydroxy-N-methyl-2-pyrrolidone and 2-hydroxy-N-methylsuccinimide in plasma},
  url          = {http://dx.doi.org/10.1007/BF02495324},
  volume       = {46},
  year         = {1997},
}