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Green Synthesis and Characterization of Gold and Silver Nanoparticles and their Application for Development of a Third Generation Lactose Biosensor

Bollella, Paolo; Schulz, Christopher LU ; Favero, Gabriele; Mazzei, Franco; Ludwig, Roland; Gorton, Lo LU and Antiochia, Riccarda (2017) In Electroanalysis 29(1). p.77-86
Abstract

In this paper we report on a facile, cost effective and environmental friendly green synthesis method of gold and silver nanoparticles (NPs) by using quercetin as reducing agent. The obtained NPs were characterized by transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS), dynamic light scattering (DLS) and UV-Vis spectroscopy and parameters such as pH, ionic strength and temperature, effectively affecting shape and size of NPs, have been carefully studied and optimized. The obtained results showed that the synthesized NPs were circular in shape with an average diameter of 5 and 8 nm for the AuNPs and the AgNPs, respectively. The “green” NPs, showing increased electroactive areas (AEA) and electronic... (More)

In this paper we report on a facile, cost effective and environmental friendly green synthesis method of gold and silver nanoparticles (NPs) by using quercetin as reducing agent. The obtained NPs were characterized by transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS), dynamic light scattering (DLS) and UV-Vis spectroscopy and parameters such as pH, ionic strength and temperature, effectively affecting shape and size of NPs, have been carefully studied and optimized. The obtained results showed that the synthesized NPs were circular in shape with an average diameter of 5 and 8 nm for the AuNPs and the AgNPs, respectively. The “green” NPs, showing increased electroactive areas (AEA) and electronic transfer rate constants (k0), were successively used to fabricate a novel third generation lactose biosensor based on cellobiose dehydrogenase from Trametes villosa (TvCDH). The TvCDH/AuNPs based lactose biosensor revealed the best results showing very efficient DET and a detection limit for lactose of 3.5 mM, a large linear range from 10 to 300 mM, a high sensitivity (5.4 μA mM−1 cm−2) and long-term stability.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cellobiose dehydrogenase, Gold nanoparticles, Green synthesis, Lactose biosensor, Silver nanoparticles
in
Electroanalysis
volume
29
issue
1
pages
10 pages
publisher
John Wiley & Sons
external identifiers
  • scopus:84990935259
  • wos:000394995900011
ISSN
1040-0397
DOI
10.1002/elan.201600476
language
English
LU publication?
yes
id
1111ef57-b38c-4df2-9f33-d9de89f6b068
date added to LUP
2017-03-21 15:22:36
date last changed
2018-01-21 04:20:25
@article{1111ef57-b38c-4df2-9f33-d9de89f6b068,
  abstract     = {<p>In this paper we report on a facile, cost effective and environmental friendly green synthesis method of gold and silver nanoparticles (NPs) by using quercetin as reducing agent. The obtained NPs were characterized by transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS), dynamic light scattering (DLS) and UV-Vis spectroscopy and parameters such as pH, ionic strength and temperature, effectively affecting shape and size of NPs, have been carefully studied and optimized. The obtained results showed that the synthesized NPs were circular in shape with an average diameter of 5 and 8 nm for the AuNPs and the AgNPs, respectively. The “green” NPs, showing increased electroactive areas (A<sub>EA</sub>) and electronic transfer rate constants (k<sup>0</sup>), were successively used to fabricate a novel third generation lactose biosensor based on cellobiose dehydrogenase from Trametes villosa (TvCDH). The TvCDH/AuNPs based lactose biosensor revealed the best results showing very efficient DET and a detection limit for lactose of 3.5 mM, a large linear range from 10 to 300 mM, a high sensitivity (5.4 μA mM<sup>−1</sup> cm<sup>−2</sup>) and long-term stability.</p>},
  author       = {Bollella, Paolo and Schulz, Christopher and Favero, Gabriele and Mazzei, Franco and Ludwig, Roland and Gorton, Lo and Antiochia, Riccarda},
  issn         = {1040-0397},
  keyword      = {Cellobiose dehydrogenase,Gold nanoparticles,Green synthesis,Lactose biosensor,Silver nanoparticles},
  language     = {eng},
  month        = {01},
  number       = {1},
  pages        = {77--86},
  publisher    = {John Wiley & Sons},
  series       = {Electroanalysis},
  title        = {Green Synthesis and Characterization of Gold and Silver Nanoparticles and their Application for Development of a Third Generation Lactose Biosensor},
  url          = {http://dx.doi.org/10.1002/elan.201600476},
  volume       = {29},
  year         = {2017},
}