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Molecular cloning and tissue distribution of cDNA encoding a novel chemoattractant-like receptor

Owman, Christer LU ; Lolait, Stephen J; Santén, Stefan LU and Olde, Björn LU (1997) In Biochemical and Biophysical Research Communications 241(2). p.390-394
Abstract
With the application of a homology screening strategy, including PCR amplification and southern blot hybridization, a novel cDNA was cloned from rat liver and anterior pituitary libraries. It was found to encode a 371-amino acid protein which has the characteristics of a heptahelix receptor and shows structural identity to members of the chemoattractant receptor family. A primary receptor message of 3.5 kb size was identified by northern blot hybridization. This RNA species showed high expression in heart and lung, while expression was lower in small intestines, colon, kidney, liver, uterus, and in brain. Another larger RNA species of 6.3 kb appeared in heart and lung. In situ hybridization histochemistry performed on tissue from liver and... (More)
With the application of a homology screening strategy, including PCR amplification and southern blot hybridization, a novel cDNA was cloned from rat liver and anterior pituitary libraries. It was found to encode a 371-amino acid protein which has the characteristics of a heptahelix receptor and shows structural identity to members of the chemoattractant receptor family. A primary receptor message of 3.5 kb size was identified by northern blot hybridization. This RNA species showed high expression in heart and lung, while expression was lower in small intestines, colon, kidney, liver, uterus, and in brain. Another larger RNA species of 6.3 kb appeared in heart and lung. In situ hybridization histochemistry performed on tissue from liver and kidney revealed a mainly vascular distribution of the receptor message. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biochemical and Biophysical Research Communications
volume
241
issue
2
pages
390 - 394
publisher
Elsevier
external identifiers
  • pmid:9425281
  • scopus:0031577757
ISSN
1090-2104
DOI
10.1006/bbrc.1997.7822
language
English
LU publication?
yes
id
26d91234-c5ee-4881-82cb-99053d6bfd4e (old id 1112047)
date added to LUP
2008-07-21 12:08:40
date last changed
2017-01-01 06:48:53
@article{26d91234-c5ee-4881-82cb-99053d6bfd4e,
  abstract     = {With the application of a homology screening strategy, including PCR amplification and southern blot hybridization, a novel cDNA was cloned from rat liver and anterior pituitary libraries. It was found to encode a 371-amino acid protein which has the characteristics of a heptahelix receptor and shows structural identity to members of the chemoattractant receptor family. A primary receptor message of 3.5 kb size was identified by northern blot hybridization. This RNA species showed high expression in heart and lung, while expression was lower in small intestines, colon, kidney, liver, uterus, and in brain. Another larger RNA species of 6.3 kb appeared in heart and lung. In situ hybridization histochemistry performed on tissue from liver and kidney revealed a mainly vascular distribution of the receptor message.},
  author       = {Owman, Christer and Lolait, Stephen J and Santén, Stefan and Olde, Björn},
  issn         = {1090-2104},
  language     = {eng},
  number       = {2},
  pages        = {390--394},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {Molecular cloning and tissue distribution of cDNA encoding a novel chemoattractant-like receptor},
  url          = {http://dx.doi.org/10.1006/bbrc.1997.7822},
  volume       = {241},
  year         = {1997},
}