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Characterization of serum antibody response to chlamydiae in patients with sexually acquired reactive arthritis

Domeika, K; Brade, L; Mårdh, Per-Anders LU ; Brade, H; Witkin, S S and Domeika, M (1997) In Pathogens and Disease 19(3). p.191-202
Abstract
Sera from patients with sexually acquired reactive arthritis (SARA) with antibodies reacting with C. trachomatis and C. pneumoniae (group 1; n = 20) and also with C. psittaci (group 2; n = 19) were analyzed for antibody specificity. Sera from group 2 reacted significantly more often with C. trachomatis serotype E, H and K and had higher antibody titers to serotype E, as tested by microimmunofluorescence tests. Cross-reactivities occurring in microimmunofluorescence tests were related to the presence of antichlamydial lipopolysaccharide antibodies, adsorption of which by recombinant lipopolysaccharide removed microimmunofluorescence reactivity with C. psittaci antigen. In group 2, significantly more sera had antibodies to C. pneumoniae,... (More)
Sera from patients with sexually acquired reactive arthritis (SARA) with antibodies reacting with C. trachomatis and C. pneumoniae (group 1; n = 20) and also with C. psittaci (group 2; n = 19) were analyzed for antibody specificity. Sera from group 2 reacted significantly more often with C. trachomatis serotype E, H and K and had higher antibody titers to serotype E, as tested by microimmunofluorescence tests. Cross-reactivities occurring in microimmunofluorescence tests were related to the presence of antichlamydial lipopolysaccharide antibodies, adsorption of which by recombinant lipopolysaccharide removed microimmunofluorescence reactivity with C. psittaci antigen. In group 2, significantly more sera had antibodies to C. pneumoniae, remaining after lipopolysaccharide adsorption, as proved by adsorption with viable C. trachomatis and C. pneumoniae organisms. None of the sera had antibodies to Yersinia enterocolitica, Shigella flexneri, Sh. sonnei and Salmonella spp. It was observed that the frequency and titer of cross-reacting antibodies to chlamydial serotypes and species were related to the time period between the diagnosis of genital chlamydial infection and of SARA. Cross-reactivities were also related to the presence of lipopolysaccharide, but not heat shock protein 60- or neutralizing antibodies to chlamydiae. Antibody reactivity induced by antichlamydial lipopolysaccharide antibodies can be removed by lipopolysaccharide adsorption. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Chlamydial hsp60, Neutralizing antibody, Lipopolysaccharide, Indirect immunofluorescence, Reactive arthritis, Chlamydial serology
in
Pathogens and Disease
volume
19
issue
3
pages
191 - 202
publisher
Wiley-Blackwell
external identifiers
  • pmid:9453389
  • scopus:0030728036
ISSN
2049-632X
DOI
10.1111/j.1574-695X.1997.tb01088.x
language
English
LU publication?
no
id
d36cef52-8fd4-44e3-a636-d48bebf57a67 (old id 1112320)
date added to LUP
2008-07-22 09:21:57
date last changed
2017-01-01 05:14:28
@article{d36cef52-8fd4-44e3-a636-d48bebf57a67,
  abstract     = {Sera from patients with sexually acquired reactive arthritis (SARA) with antibodies reacting with C. trachomatis and C. pneumoniae (group 1; n = 20) and also with C. psittaci (group 2; n = 19) were analyzed for antibody specificity. Sera from group 2 reacted significantly more often with C. trachomatis serotype E, H and K and had higher antibody titers to serotype E, as tested by microimmunofluorescence tests. Cross-reactivities occurring in microimmunofluorescence tests were related to the presence of antichlamydial lipopolysaccharide antibodies, adsorption of which by recombinant lipopolysaccharide removed microimmunofluorescence reactivity with C. psittaci antigen. In group 2, significantly more sera had antibodies to C. pneumoniae, remaining after lipopolysaccharide adsorption, as proved by adsorption with viable C. trachomatis and C. pneumoniae organisms. None of the sera had antibodies to Yersinia enterocolitica, Shigella flexneri, Sh. sonnei and Salmonella spp. It was observed that the frequency and titer of cross-reacting antibodies to chlamydial serotypes and species were related to the time period between the diagnosis of genital chlamydial infection and of SARA. Cross-reactivities were also related to the presence of lipopolysaccharide, but not heat shock protein 60- or neutralizing antibodies to chlamydiae. Antibody reactivity induced by antichlamydial lipopolysaccharide antibodies can be removed by lipopolysaccharide adsorption.},
  author       = {Domeika, K and Brade, L and Mårdh, Per-Anders and Brade, H and Witkin, S S and Domeika, M},
  issn         = {2049-632X},
  keyword      = {Chlamydial hsp60,Neutralizing antibody,Lipopolysaccharide,Indirect immunofluorescence,Reactive arthritis,Chlamydial serology},
  language     = {eng},
  number       = {3},
  pages        = {191--202},
  publisher    = {Wiley-Blackwell},
  series       = {Pathogens and Disease},
  title        = {Characterization of serum antibody response to chlamydiae in patients with sexually acquired reactive arthritis},
  url          = {http://dx.doi.org/10.1111/j.1574-695X.1997.tb01088.x},
  volume       = {19},
  year         = {1997},
}