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DNA fragmentation and ultrastructural changes of degenerating cells in atherosclerotic lesions and smooth muscle cells exposed to oxidized LDL in vitro

Jovinge, Stefan LU ; Crisby, M; Thyberg, J and Nilsson, J (1997) In Arteriosclerosis, Thrombosis and Vascular Biology 17(10). p.2225-2231
Abstract
Degeneration of smooth muscle cells in the fibrous cap of atherosclerotic lesions is an important factor in plaque rupture. Recent studies have suggested that many plaque cells are in a process of apoptosis as determined by positive deoxyribonucleotide-transferase-mediated dUTP end labeling. In this study, we demonstrate the existence of a colocalization between deoxyribonucleotide-transferase-mediated dUTP end labeling-positive smooth muscle cells and oxidized LDL immunoreactivity in human carotid plaques. Oxidized LDL was found to induce deoxyribonucleotide-transferase-mediated dUTP end labeling positivity in cultured human smooth muscle cells, but only in the presence of tumor necrosis factor-alpha and interferon-gamma. Electron... (More)
Degeneration of smooth muscle cells in the fibrous cap of atherosclerotic lesions is an important factor in plaque rupture. Recent studies have suggested that many plaque cells are in a process of apoptosis as determined by positive deoxyribonucleotide-transferase-mediated dUTP end labeling. In this study, we demonstrate the existence of a colocalization between deoxyribonucleotide-transferase-mediated dUTP end labeling-positive smooth muscle cells and oxidized LDL immunoreactivity in human carotid plaques. Oxidized LDL was found to induce deoxyribonucleotide-transferase-mediated dUTP end labeling positivity in cultured human smooth muscle cells, but only in the presence of tumor necrosis factor-alpha and interferon-gamma. Electron microscopic analysis of cultured smooth muscle cells exposed to oxidized LDL in the absence of cytokines demonstrated cytoplasmic swelling and disruption of the plasma membrane, suggesting cell death by oncosis. Cells exposed to both oxidized LDL and cytokines were characterized by chromatin and cytoplasmic condensation compatible with cell death by apoptosis. These findings further support the notion that oxidized lipids play a role in plaque cell death. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
apoptosis, cell death, oxidized LDL, TNF{alpha}, {gamma}IFN
in
Arteriosclerosis, Thrombosis and Vascular Biology
volume
17
issue
10
pages
2225 - 2231
publisher
American Heart Association
external identifiers
  • pmid:9351393
  • scopus:0031456718
ISSN
1524-4636
language
English
LU publication?
no
id
62d51113-cd03-44c8-b6be-9eec30bc8094 (old id 1112575)
alternative location
http://atvb.ahajournals.org/cgi/content/full/17/10/2225
date added to LUP
2008-07-17 08:59:02
date last changed
2017-03-05 03:38:32
@article{62d51113-cd03-44c8-b6be-9eec30bc8094,
  abstract     = {Degeneration of smooth muscle cells in the fibrous cap of atherosclerotic lesions is an important factor in plaque rupture. Recent studies have suggested that many plaque cells are in a process of apoptosis as determined by positive deoxyribonucleotide-transferase-mediated dUTP end labeling. In this study, we demonstrate the existence of a colocalization between deoxyribonucleotide-transferase-mediated dUTP end labeling-positive smooth muscle cells and oxidized LDL immunoreactivity in human carotid plaques. Oxidized LDL was found to induce deoxyribonucleotide-transferase-mediated dUTP end labeling positivity in cultured human smooth muscle cells, but only in the presence of tumor necrosis factor-alpha and interferon-gamma. Electron microscopic analysis of cultured smooth muscle cells exposed to oxidized LDL in the absence of cytokines demonstrated cytoplasmic swelling and disruption of the plasma membrane, suggesting cell death by oncosis. Cells exposed to both oxidized LDL and cytokines were characterized by chromatin and cytoplasmic condensation compatible with cell death by apoptosis. These findings further support the notion that oxidized lipids play a role in plaque cell death.},
  author       = {Jovinge, Stefan and Crisby, M and Thyberg, J and Nilsson, J},
  issn         = {1524-4636},
  keyword      = {apoptosis,cell death,oxidized LDL,TNF{alpha},{gamma}IFN},
  language     = {eng},
  number       = {10},
  pages        = {2225--2231},
  publisher    = {American Heart Association},
  series       = {Arteriosclerosis, Thrombosis and Vascular Biology},
  title        = {DNA fragmentation and ultrastructural changes of degenerating cells in atherosclerotic lesions and smooth muscle cells exposed to oxidized LDL in vitro},
  volume       = {17},
  year         = {1997},
}