Advanced

The glutathione levels are reduced in Goto-Kakizaki rat retina, but are not influenced by aminoguanidine treatment

Agardh, Carl-David LU ; Agardh, Elisabet LU ; Hultberg, Björn LU ; Qian, Yuning LU and Ostenson, Claes-Göran (1998) In Current Eye Research 17(3). p.251-256
Abstract
PURPOSE: To examine the levels of the free radical protecting enzyme glutathione and the endothelial/pericyte ratio in retinal capillaries in the Goto-Kakizaki (GK) Wistar rat, with and without aminoguanidine treatment. METHODS: Eight-month-old GK rats, with non-obese, spontaneous non-insulin-dependent diabetes mellitus (NIDDM), were examined after a six month period of aminoguanidine treatment. Glutathione levels were measured with high performance liquid chromatography and the endothelial/pericyte ratio was calculated in trypsin digested vessel preparations. RESULTS: The levels of glutathione in GK rat retina were significantly lower compared to controls (p = 0.0108). There was no difference in the endothelial/pericyte ratio compared to... (More)
PURPOSE: To examine the levels of the free radical protecting enzyme glutathione and the endothelial/pericyte ratio in retinal capillaries in the Goto-Kakizaki (GK) Wistar rat, with and without aminoguanidine treatment. METHODS: Eight-month-old GK rats, with non-obese, spontaneous non-insulin-dependent diabetes mellitus (NIDDM), were examined after a six month period of aminoguanidine treatment. Glutathione levels were measured with high performance liquid chromatography and the endothelial/pericyte ratio was calculated in trypsin digested vessel preparations. RESULTS: The levels of glutathione in GK rat retina were significantly lower compared to controls (p = 0.0108). There was no difference in the endothelial/pericyte ratio compared to matched control rats (1.8 +/- 0.2 vs. 1.8 +/- 0.1, respectively). Aminoguanidine treatment did not influence either the degree of hyperglycemia, the levels of glutathione or the endothelial/pericyte ratio in GK or control rat retina. CONCLUSIONS: The results indicate that impaired glucose metabolism may influence one of the defense mechanisms for oxidative stress, but also suggest that decreased glutathione levels occur prior to morphological signs of pericyte loss and/or endothelial cell proliferation in this animal model of hereditary NIDDM. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Current Eye Research
volume
17
issue
3
pages
251 - 256
publisher
Taylor & Francis
external identifiers
  • pmid:9543633
  • scopus:0031907365
ISSN
0271-3683
DOI
10.1076/ceyr.17.3.251.5217
language
English
LU publication?
yes
id
6cf62629-1824-4492-8d94-9b417153692e (old id 1112655)
date added to LUP
2008-07-10 11:22:18
date last changed
2017-01-01 04:48:44
@article{6cf62629-1824-4492-8d94-9b417153692e,
  abstract     = {PURPOSE: To examine the levels of the free radical protecting enzyme glutathione and the endothelial/pericyte ratio in retinal capillaries in the Goto-Kakizaki (GK) Wistar rat, with and without aminoguanidine treatment. METHODS: Eight-month-old GK rats, with non-obese, spontaneous non-insulin-dependent diabetes mellitus (NIDDM), were examined after a six month period of aminoguanidine treatment. Glutathione levels were measured with high performance liquid chromatography and the endothelial/pericyte ratio was calculated in trypsin digested vessel preparations. RESULTS: The levels of glutathione in GK rat retina were significantly lower compared to controls (p = 0.0108). There was no difference in the endothelial/pericyte ratio compared to matched control rats (1.8 +/- 0.2 vs. 1.8 +/- 0.1, respectively). Aminoguanidine treatment did not influence either the degree of hyperglycemia, the levels of glutathione or the endothelial/pericyte ratio in GK or control rat retina. CONCLUSIONS: The results indicate that impaired glucose metabolism may influence one of the defense mechanisms for oxidative stress, but also suggest that decreased glutathione levels occur prior to morphological signs of pericyte loss and/or endothelial cell proliferation in this animal model of hereditary NIDDM.},
  author       = {Agardh, Carl-David and Agardh, Elisabet and Hultberg, Björn and Qian, Yuning and Ostenson, Claes-Göran},
  issn         = {0271-3683},
  language     = {eng},
  number       = {3},
  pages        = {251--256},
  publisher    = {Taylor & Francis},
  series       = {Current Eye Research},
  title        = {The glutathione levels are reduced in Goto-Kakizaki rat retina, but are not influenced by aminoguanidine treatment},
  url          = {http://dx.doi.org/10.1076/ceyr.17.3.251.5217},
  volume       = {17},
  year         = {1998},
}