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Abnormal DNA damage-inducible protein in cells from Sjogren's syndrome patients

Lavasani, Shahram LU ; Henriksson, Gunnel LU ; Brant, Marta LU ; Henriksson, Agnethe; Radulic, M; Manthorpe, Rolf LU and Bredberg, Anders LU (1998) In Journal of Autoimmunity 11(4). p.363-369
Abstract
Antinuclear antibodies are commonly found in patients with Sjogren's syndrome. It has been suggested that the development of antinuclear antibodies depends on the activation of the spliceosome and other transcription-related subcellular particles, some of which have recently been shown also to function in DNA-modifying processes, such as DNA repair and V(D)J recombination. These observations add weight to a previously proposed model for the aetiology of Sjogren's syndrome. This includes the abnormal processing of the T-cell receptor and immunoglobulin genes. To test this hypothesis further, the present study on DNA-modifying proteins in Sjogren's syndrome was initiated. Gel-shift experiments using protein extracted from UV-treated Sjogren... (More)
Antinuclear antibodies are commonly found in patients with Sjogren's syndrome. It has been suggested that the development of antinuclear antibodies depends on the activation of the spliceosome and other transcription-related subcellular particles, some of which have recently been shown also to function in DNA-modifying processes, such as DNA repair and V(D)J recombination. These observations add weight to a previously proposed model for the aetiology of Sjogren's syndrome. This includes the abnormal processing of the T-cell receptor and immunoglobulin genes. To test this hypothesis further, the present study on DNA-modifying proteins in Sjogren's syndrome was initiated. Gel-shift experiments using protein extracted from UV-treated Sjogren cells provided evidence of high molecular weight DNA-binding protein in six out of 12 Sjogren patients studied (but not among seven healthy controls). Some Sjogren sera displayed antibodies to protein extracts from cells treated with psoralen plus UVA radiation. These results indicate an abnormal DNA damage-inducible response in Sjogren's syndrome. It may therefore be concluded that alterations in nuclear protein may play a role in the aetiology of Sjogren's syndrome. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
antinuclear antibodies, DNA damage, DNA-binding protein, Sjögren's syndrome
in
Journal of Autoimmunity
volume
11
issue
4
pages
363 - 369
publisher
Elsevier
external identifiers
  • pmid:9776714
  • scopus:0031783913
ISSN
0896-8411
DOI
10.1006/jaut.1998.0211
language
English
LU publication?
yes
id
32764342-572b-4a1f-848a-f4ca975e7b0c (old id 1112700)
date added to LUP
2008-07-11 09:15:05
date last changed
2017-01-01 04:57:18
@article{32764342-572b-4a1f-848a-f4ca975e7b0c,
  abstract     = {Antinuclear antibodies are commonly found in patients with Sjogren's syndrome. It has been suggested that the development of antinuclear antibodies depends on the activation of the spliceosome and other transcription-related subcellular particles, some of which have recently been shown also to function in DNA-modifying processes, such as DNA repair and V(D)J recombination. These observations add weight to a previously proposed model for the aetiology of Sjogren's syndrome. This includes the abnormal processing of the T-cell receptor and immunoglobulin genes. To test this hypothesis further, the present study on DNA-modifying proteins in Sjogren's syndrome was initiated. Gel-shift experiments using protein extracted from UV-treated Sjogren cells provided evidence of high molecular weight DNA-binding protein in six out of 12 Sjogren patients studied (but not among seven healthy controls). Some Sjogren sera displayed antibodies to protein extracts from cells treated with psoralen plus UVA radiation. These results indicate an abnormal DNA damage-inducible response in Sjogren's syndrome. It may therefore be concluded that alterations in nuclear protein may play a role in the aetiology of Sjogren's syndrome.},
  author       = {Lavasani, Shahram and Henriksson, Gunnel and Brant, Marta and Henriksson, Agnethe and Radulic, M and Manthorpe, Rolf and Bredberg, Anders},
  issn         = {0896-8411},
  keyword      = {antinuclear antibodies,DNA damage,DNA-binding protein,Sjögren's syndrome},
  language     = {eng},
  number       = {4},
  pages        = {363--369},
  publisher    = {Elsevier},
  series       = {Journal of Autoimmunity},
  title        = {Abnormal DNA damage-inducible protein in cells from Sjogren's syndrome patients},
  url          = {http://dx.doi.org/10.1006/jaut.1998.0211},
  volume       = {11},
  year         = {1998},
}