Insulin-induced translocation of protein kinase B to the plasma membrane in rat adipocytes
Göransson, Olga LU
; Wijkander, Jonny
; Manganiello, V
and Degerman, Eva
LU
(1998)
In Biochemical and Biophysical Research Communications
246(1).
p.249-254
- Abstract
- Protein kinase B (PKB) has previously been shown to be activated in response to insulin and growth factor stimulation. The activation mechanism has been suggested to involve translocation of PKB to membranes, where it is phosphorylated and activated. Insulin-induced translocation of PKB has not been demonstrated in a physiological target cell. Therefore we have used the primary rat adipocyte to investigate insulin-induced translocation of PKB. In the presence of 1 nM insulin translocation of PKB was detected within 30 seconds and was blocked by wortmannin, a selective phosphatidylinositol 3-kinase inhibitor. This translocation was potentiated by the tyrosine phosphatase inhibitor vanadate. Subcellular localization studies revealed that PKB... (More)
- Protein kinase B (PKB) has previously been shown to be activated in response to insulin and growth factor stimulation. The activation mechanism has been suggested to involve translocation of PKB to membranes, where it is phosphorylated and activated. Insulin-induced translocation of PKB has not been demonstrated in a physiological target cell. Therefore we have used the primary rat adipocyte to investigate insulin-induced translocation of PKB. In the presence of 1 nM insulin translocation of PKB was detected within 30 seconds and was blocked by wortmannin, a selective phosphatidylinositol 3-kinase inhibitor. This translocation was potentiated by the tyrosine phosphatase inhibitor vanadate. Subcellular localization studies revealed that PKB translocated to the plasma membrane. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1112738
- author
- Göransson, Olga
LU
; Wijkander, Jonny
; Manganiello, V
and Degerman, Eva
LU
- organization
- publishing date
- 1998
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- insulin, adipocyte, protein kinase B, plasma membrane, phosphatidylinositol 3-kinase
- in
- Biochemical and Biophysical Research Communications
- volume
- 246
- issue
- 1
- pages
- 249 - 254
- publisher
- Elsevier
- external identifiers
-
- pmid:9600101
- scopus:0344157383
- ISSN
- 1090-2104
- DOI
- 10.1006/bbrc.1998.8602
- language
- English
- LU publication?
- yes
- id
- 8a6db71c-f935-4b90-bf6b-c8fd45dab84d (old id 1112738)
- date added to LUP
- 2016-04-01 16:48:21
- date last changed
- 2024-04-12 07:51:12
@article{8a6db71c-f935-4b90-bf6b-c8fd45dab84d,
abstract = {{Protein kinase B (PKB) has previously been shown to be activated in response to insulin and growth factor stimulation. The activation mechanism has been suggested to involve translocation of PKB to membranes, where it is phosphorylated and activated. Insulin-induced translocation of PKB has not been demonstrated in a physiological target cell. Therefore we have used the primary rat adipocyte to investigate insulin-induced translocation of PKB. In the presence of 1 nM insulin translocation of PKB was detected within 30 seconds and was blocked by wortmannin, a selective phosphatidylinositol 3-kinase inhibitor. This translocation was potentiated by the tyrosine phosphatase inhibitor vanadate. Subcellular localization studies revealed that PKB translocated to the plasma membrane.}},
author = {{Göransson, Olga and Wijkander, Jonny and Manganiello, V and Degerman, Eva}},
issn = {{1090-2104}},
keywords = {{insulin; adipocyte; protein kinase B; plasma membrane; phosphatidylinositol 3-kinase}},
language = {{eng}},
number = {{1}},
pages = {{249--254}},
publisher = {{Elsevier}},
series = {{Biochemical and Biophysical Research Communications}},
title = {{Insulin-induced translocation of protein kinase B to the plasma membrane in rat adipocytes}},
url = {{http://dx.doi.org/10.1006/bbrc.1998.8602}},
doi = {{10.1006/bbrc.1998.8602}},
volume = {{246}},
year = {{1998}},
}