GBV-C/HGV infection in hepatitis C virus-infected deferred Swedish blood donors
(1998) In Journal of Medical Virology 54(2). p.75-79- Abstract
- Sera from 62 hepatitis C virus (HCV)-infected Swedish blood donors were tested by a nested polymerase chain reaction using primers targeting the 5'-noncoding region of the GB virus-C/hepatitis G (GBV-C/HGV) genome and an enzyme-linked immunosorbent assay that detects antibodies to the envelope protein E2 of GBV-C/HGV (anti-E2). Fourteen (22%) and 21 (34%) of the 62 blood donors were found to be GBV-C/HGV RNA and anti-E2 positive, respectively. None of the blood donors was positive for both GBV-C/HGV RNA and anti-E2. Thus, 35 of 62 (56%) HCV-infected donors had been exposed to GBV-C/HGV infection. At sequencing of the 14 GBV-C/HGV isolates, 12 were identified as subtype 2a and 2 as subtype 2b. One of 7 (14%) donors with mild liver disease... (More)
- Sera from 62 hepatitis C virus (HCV)-infected Swedish blood donors were tested by a nested polymerase chain reaction using primers targeting the 5'-noncoding region of the GB virus-C/hepatitis G (GBV-C/HGV) genome and an enzyme-linked immunosorbent assay that detects antibodies to the envelope protein E2 of GBV-C/HGV (anti-E2). Fourteen (22%) and 21 (34%) of the 62 blood donors were found to be GBV-C/HGV RNA and anti-E2 positive, respectively. None of the blood donors was positive for both GBV-C/HGV RNA and anti-E2. Thus, 35 of 62 (56%) HCV-infected donors had been exposed to GBV-C/HGV infection. At sequencing of the 14 GBV-C/HGV isolates, 12 were identified as subtype 2a and 2 as subtype 2b. One of 7 (14%) donors with mild liver disease such as steatosis and nonspecific reactive hepatitis had been exposed to GBV-C/HGV vs. 34 of 55 (62%) with chronic hepatitis with or without cirrhosis (P = 0.04). All other differences in histology were small between HCV and dual HCV GBV-C/HGV-infected donors. In conclusion, more than half of HCV-infected Swedish blood donors in this study were positive for either GBV-C/HGV RNA or anti-E2. GBV-C/HGV viremia and seropositivity were mutually exclusive. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1112751
- author
- Shev, S ; Björkman, Per LU ; Norkrans, G ; Foberg, U ; Fryden, A ; Lindh, G ; Lindholm, A ; Weiland, O and Widell, Anders LU
- organization
- publishing date
- 1998
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- HGV, HCV, GBV-C, anti-E2, blood donors, chronic hepatitis
- in
- Journal of Medical Virology
- volume
- 54
- issue
- 2
- pages
- 75 - 79
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:9496362
- scopus:2642659396
- ISSN
- 1096-9071
- DOI
- 10.1002/(SICI)1096-9071(199802)54:2<75::AID-JMV1>3.0.CO;2-L
- language
- English
- LU publication?
- yes
- id
- b6c11551-511b-4adb-a3bc-a502ebab965e (old id 1112751)
- date added to LUP
- 2016-04-01 11:48:13
- date last changed
- 2022-01-26 18:30:10
@article{b6c11551-511b-4adb-a3bc-a502ebab965e, abstract = {{Sera from 62 hepatitis C virus (HCV)-infected Swedish blood donors were tested by a nested polymerase chain reaction using primers targeting the 5'-noncoding region of the GB virus-C/hepatitis G (GBV-C/HGV) genome and an enzyme-linked immunosorbent assay that detects antibodies to the envelope protein E2 of GBV-C/HGV (anti-E2). Fourteen (22%) and 21 (34%) of the 62 blood donors were found to be GBV-C/HGV RNA and anti-E2 positive, respectively. None of the blood donors was positive for both GBV-C/HGV RNA and anti-E2. Thus, 35 of 62 (56%) HCV-infected donors had been exposed to GBV-C/HGV infection. At sequencing of the 14 GBV-C/HGV isolates, 12 were identified as subtype 2a and 2 as subtype 2b. One of 7 (14%) donors with mild liver disease such as steatosis and nonspecific reactive hepatitis had been exposed to GBV-C/HGV vs. 34 of 55 (62%) with chronic hepatitis with or without cirrhosis (P = 0.04). All other differences in histology were small between HCV and dual HCV GBV-C/HGV-infected donors. In conclusion, more than half of HCV-infected Swedish blood donors in this study were positive for either GBV-C/HGV RNA or anti-E2. GBV-C/HGV viremia and seropositivity were mutually exclusive.}}, author = {{Shev, S and Björkman, Per and Norkrans, G and Foberg, U and Fryden, A and Lindh, G and Lindholm, A and Weiland, O and Widell, Anders}}, issn = {{1096-9071}}, keywords = {{HGV; HCV; GBV-C; anti-E2; blood donors; chronic hepatitis}}, language = {{eng}}, number = {{2}}, pages = {{75--79}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Journal of Medical Virology}}, title = {{GBV-C/HGV infection in hepatitis C virus-infected deferred Swedish blood donors}}, url = {{http://dx.doi.org/10.1002/(SICI)1096-9071(199802)54:2<75::AID-JMV1>3.0.CO;2-L}}, doi = {{10.1002/(SICI)1096-9071(199802)54:2<75::AID-JMV1>3.0.CO;2-L}}, volume = {{54}}, year = {{1998}}, }