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S-phase fraction assessed by a variant of the rectangular model adapted to the flow-cytometric DNA histogram profile

Stal, Olle and Baldetorp, Bo LU (1998) In Cytometry 33(4). p.487-491
Abstract
Measurement of S-phase fraction by DNA flow cytometry is widely used in the analysis of human tumors. The calculation of fraction of S-phase cells is performed either by means of curve-fitting techniques or by more simple planimetric methods. Estimates by the latter often suffer from subjectivity or poor adaptation to complex DNA histograms. We describe a variant which is based on the rectangular model. The height of the rectangle is automatically determined from the lowest density value in the S-phase interval. The variant was tested on 141 DNA histograms, and the results were compared with those obtained with other variants based on the rectangular model. The new variant is thought to circumvent some of the problems concerning... (More)
Measurement of S-phase fraction by DNA flow cytometry is widely used in the analysis of human tumors. The calculation of fraction of S-phase cells is performed either by means of curve-fitting techniques or by more simple planimetric methods. Estimates by the latter often suffer from subjectivity or poor adaptation to complex DNA histograms. We describe a variant which is based on the rectangular model. The height of the rectangle is automatically determined from the lowest density value in the S-phase interval. The variant was tested on 141 DNA histograms, and the results were compared with those obtained with other variants based on the rectangular model. The new variant is thought to circumvent some of the problems concerning subjectivity and disturbances caused by aggregate peaks. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
DNA, flow cytometry, S phase, rectangular model
in
Cytometry
volume
33
issue
4
pages
487 - 491
publisher
John Wiley & Sons
external identifiers
  • pmid:9845445
  • scopus:0032403373
ISSN
0196-4763
DOI
10.1002/(SICI)1097-0320(19981201)33:4<487::AID-CYTO14>3.0.CO;2-1
language
English
LU publication?
yes
id
a7ab9165-ce80-4a45-9236-ce3f0b62c168 (old id 1112826)
date added to LUP
2008-07-11 11:53:49
date last changed
2017-01-01 06:52:03
@article{a7ab9165-ce80-4a45-9236-ce3f0b62c168,
  abstract     = {Measurement of S-phase fraction by DNA flow cytometry is widely used in the analysis of human tumors. The calculation of fraction of S-phase cells is performed either by means of curve-fitting techniques or by more simple planimetric methods. Estimates by the latter often suffer from subjectivity or poor adaptation to complex DNA histograms. We describe a variant which is based on the rectangular model. The height of the rectangle is automatically determined from the lowest density value in the S-phase interval. The variant was tested on 141 DNA histograms, and the results were compared with those obtained with other variants based on the rectangular model. The new variant is thought to circumvent some of the problems concerning subjectivity and disturbances caused by aggregate peaks.},
  author       = {Stal, Olle and Baldetorp, Bo},
  issn         = {0196-4763},
  keyword      = {DNA,flow cytometry,S phase,rectangular model},
  language     = {eng},
  number       = {4},
  pages        = {487--491},
  publisher    = {John Wiley & Sons},
  series       = {Cytometry},
  title        = {S-phase fraction assessed by a variant of the rectangular model adapted to the flow-cytometric DNA histogram profile},
  url          = {http://dx.doi.org/10.1002/(SICI)1097-0320(19981201)33:4<487::AID-CYTO14>3.0.CO;2-1},
  volume       = {33},
  year         = {1998},
}