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Comparison of different labelling index formulae used on bromodeoxyuridine-flow cytometry data

Johansson, Maria C LU ; Johansson, Roland; Baldetorp, Bo LU and Oredsson, Stina LU (1998) In Cytometry 32(3). p.233-240
Abstract
The essence of the bromodeoxyuridine (BrdUrd)-flow cytometry (FCM) technique is that cells are labelled with the thymidine analogue BrdUrd. They are then allowed to progress through the cell cycle in a BrdUrd-free environment during the postlabelling time period. At a postlabelling time shorter than the length of the S phase (Ts), cells are fixed and prepared for FCM-mediated analysis of BrdUrd and DNA contents. From FCM-derived data, cell cycle kinetic parameters such as labelling index (LI), Ts, and potential doubling time (Tpot) can be calculated. Tpot is believed to be important in the evaluation of tumor aggressiveness and therapy response. Since LI is most commonly used together with Ts to calculate Tpot, it is important that both LI... (More)
The essence of the bromodeoxyuridine (BrdUrd)-flow cytometry (FCM) technique is that cells are labelled with the thymidine analogue BrdUrd. They are then allowed to progress through the cell cycle in a BrdUrd-free environment during the postlabelling time period. At a postlabelling time shorter than the length of the S phase (Ts), cells are fixed and prepared for FCM-mediated analysis of BrdUrd and DNA contents. From FCM-derived data, cell cycle kinetic parameters such as labelling index (LI), Ts, and potential doubling time (Tpot) can be calculated. Tpot is believed to be important in the evaluation of tumor aggressiveness and therapy response. Since LI is most commonly used together with Ts to calculate Tpot, it is important that both LI and Ts are independent of the time when cells are sampled. Several formulae to calculate LI and Ts have been presented. In the present paper, we deal with various formulae to calculate LI. These formulae differ in how they take into account unlabelled and BrdUrd-labelled cells in various fractions of the cell cycle. We present a new formula, which takes into consideration cells in the different fractions and thus makes LI theoretically independent of postlabelling time. Our results show that different LI values are obtained when different formulae are used to calculate LI. In addition, we show that the BrdUrd labelling period should be kept as short as possible. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
labelling index, bromodeoxyuridine, flow cytometry, cell kinetics
in
Cytometry
volume
32
issue
3
pages
233 - 240
publisher
John Wiley & Sons
external identifiers
  • pmid:9667513
  • scopus:0032125519
ISSN
0196-4763
DOI
10.1002/(SICI)1097-0320(19980701)32:3<233::AID-CYTO10>3.0.CO;2-O
language
English
LU publication?
yes
id
991edefe-d932-451f-8117-edbdb8f3d4bd (old id 1112841)
date added to LUP
2008-07-11 13:07:01
date last changed
2017-01-01 06:37:32
@article{991edefe-d932-451f-8117-edbdb8f3d4bd,
  abstract     = {The essence of the bromodeoxyuridine (BrdUrd)-flow cytometry (FCM) technique is that cells are labelled with the thymidine analogue BrdUrd. They are then allowed to progress through the cell cycle in a BrdUrd-free environment during the postlabelling time period. At a postlabelling time shorter than the length of the S phase (Ts), cells are fixed and prepared for FCM-mediated analysis of BrdUrd and DNA contents. From FCM-derived data, cell cycle kinetic parameters such as labelling index (LI), Ts, and potential doubling time (Tpot) can be calculated. Tpot is believed to be important in the evaluation of tumor aggressiveness and therapy response. Since LI is most commonly used together with Ts to calculate Tpot, it is important that both LI and Ts are independent of the time when cells are sampled. Several formulae to calculate LI and Ts have been presented. In the present paper, we deal with various formulae to calculate LI. These formulae differ in how they take into account unlabelled and BrdUrd-labelled cells in various fractions of the cell cycle. We present a new formula, which takes into consideration cells in the different fractions and thus makes LI theoretically independent of postlabelling time. Our results show that different LI values are obtained when different formulae are used to calculate LI. In addition, we show that the BrdUrd labelling period should be kept as short as possible.},
  author       = {Johansson, Maria C and Johansson, Roland and Baldetorp, Bo and Oredsson, Stina},
  issn         = {0196-4763},
  keyword      = {labelling index,bromodeoxyuridine,flow cytometry,cell kinetics},
  language     = {eng},
  number       = {3},
  pages        = {233--240},
  publisher    = {John Wiley & Sons},
  series       = {Cytometry},
  title        = {Comparison of different labelling index formulae used on bromodeoxyuridine-flow cytometry data},
  url          = {http://dx.doi.org/10.1002/(SICI)1097-0320(19980701)32:3<233::AID-CYTO10>3.0.CO;2-O},
  volume       = {32},
  year         = {1998},
}