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Complex karyotypes in flow cytometrically DNA-diploid squamous cell carcinomas of the head and neck

Åkervall, Jan LU ; Jin, Yuesheng LU ; Baldetorp, Bo LU ; Mertens, Fredrik LU and Wennerberg, Johan LU (1998) In British Journal of Cancer 77(7). p.1082-1088
Abstract
In squamous cell carcinoma of the head and neck (SCCHN), DNA ploidy as determined by flow cytometry (FCM) has been found to yield prognostic information but only for tumours at oral sites. Cytogenetic findings have indicated complex karyotype to be a correlate of poor clinical outcome. In the present study, 73 SCCHN were investigated with the two techniques. Aneuploid cell populations were identified in 49 (67%) cases by FCM but in only 21 (29%) cases by cytogenetic analysis. The chromosome index (CI), calculated as the mean chromosome number divided by 46, was compared with the respective DNA index (DI) obtained by FCM in 15 tumours, non-diploid according to both techniques, DI being systematically 12% higher than CI in this subgroup.... (More)
In squamous cell carcinoma of the head and neck (SCCHN), DNA ploidy as determined by flow cytometry (FCM) has been found to yield prognostic information but only for tumours at oral sites. Cytogenetic findings have indicated complex karyotype to be a correlate of poor clinical outcome. In the present study, 73 SCCHN were investigated with the two techniques. Aneuploid cell populations were identified in 49 (67%) cases by FCM but in only 21 (29%) cases by cytogenetic analysis. The chromosome index (CI), calculated as the mean chromosome number divided by 46, was compared with the respective DNA index (DI) obtained by FCM in 15 tumours, non-diploid according to both techniques, DI being systematically 12% higher than CI in this subgroup. Eight (33%) of the 24 tumours diploid according to FCM had complex karyotypes, three of the tumours being cytogenetically hypodiploid, three diploid and two non-diploid. The findings in the present study may partly explain the low prognostic value of ploidy status as assessed by FCM that has been observed in SCCHN. In addition, we conclude that FCM yields information of the genetic changes that is too unspecific, and that cytogenetic analysis shows a high rate of unsuccessful investigations, thus diminishing the value of the two methods as prognostic factors in SCCHN. (Less)
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author
organization
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Contribution to journal
publication status
published
subject
in
British Journal of Cancer
volume
77
issue
7
pages
1082 - 1088
publisher
Nature Publishing Group
external identifiers
  • pmid:9569043
  • scopus:0031882359
ISSN
1532-1827
language
English
LU publication?
yes
id
d82f088d-71a0-4920-a61d-0951b44dc3b4 (old id 1112860)
date added to LUP
2008-07-11 13:40:19
date last changed
2017-01-01 04:53:48
@article{d82f088d-71a0-4920-a61d-0951b44dc3b4,
  abstract     = {In squamous cell carcinoma of the head and neck (SCCHN), DNA ploidy as determined by flow cytometry (FCM) has been found to yield prognostic information but only for tumours at oral sites. Cytogenetic findings have indicated complex karyotype to be a correlate of poor clinical outcome. In the present study, 73 SCCHN were investigated with the two techniques. Aneuploid cell populations were identified in 49 (67%) cases by FCM but in only 21 (29%) cases by cytogenetic analysis. The chromosome index (CI), calculated as the mean chromosome number divided by 46, was compared with the respective DNA index (DI) obtained by FCM in 15 tumours, non-diploid according to both techniques, DI being systematically 12% higher than CI in this subgroup. Eight (33%) of the 24 tumours diploid according to FCM had complex karyotypes, three of the tumours being cytogenetically hypodiploid, three diploid and two non-diploid. The findings in the present study may partly explain the low prognostic value of ploidy status as assessed by FCM that has been observed in SCCHN. In addition, we conclude that FCM yields information of the genetic changes that is too unspecific, and that cytogenetic analysis shows a high rate of unsuccessful investigations, thus diminishing the value of the two methods as prognostic factors in SCCHN.},
  author       = {Åkervall, Jan and Jin, Yuesheng and Baldetorp, Bo and Mertens, Fredrik and Wennerberg, Johan},
  issn         = {1532-1827},
  language     = {eng},
  number       = {7},
  pages        = {1082--1088},
  publisher    = {Nature Publishing Group},
  series       = {British Journal of Cancer},
  title        = {Complex karyotypes in flow cytometrically DNA-diploid squamous cell carcinomas of the head and neck},
  volume       = {77},
  year         = {1998},
}