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A TCR alpha chain transgene induces maturation of CD4- CD8- alpha beta+ T cells from gamma delta T cell precursors

Fritsch, Maria; Andersson, Åsa LU ; Leandersson, Karin LU and Ivars, Fredrik LU (1998) In European Journal of Immunology 28(3). p.828-837
Abstract
The proportion of CD4- CD8- double-negative (DN) alpha beta T cells is increased both in the thymus and in peripheral lymphoid organs of TCR alpha chain-transgenic mice. In this report we have characterized this T cell population to elucidate its relationship to alpha beta and gamma delta T cells. We show that the transgenic DN cells are phenotypically similar to gamma delta T cells but distinct from DN NK T cells. The precursors of DN cells have neither rearranged endogenous TCR alpha genes nor been negatively selected by the MIsa antigen, suggesting that they originate from a differentiation stage before the onset of TCR alpha chain rearrangements and CD4/CD8 gene expression. Neither in-frame V delta D delta J delta nor V gamma J gamma... (More)
The proportion of CD4- CD8- double-negative (DN) alpha beta T cells is increased both in the thymus and in peripheral lymphoid organs of TCR alpha chain-transgenic mice. In this report we have characterized this T cell population to elucidate its relationship to alpha beta and gamma delta T cells. We show that the transgenic DN cells are phenotypically similar to gamma delta T cells but distinct from DN NK T cells. The precursors of DN cells have neither rearranged endogenous TCR alpha genes nor been negatively selected by the MIsa antigen, suggesting that they originate from a differentiation stage before the onset of TCR alpha chain rearrangements and CD4/CD8 gene expression. Neither in-frame V delta D delta J delta nor V gamma J gamma rearrangements are over-represented in this population. However, since peripheral gamma delta T cells with functional TCR beta gene rearrangements have been depleted in the transgenics, we propose that the transgenic DN population, at least partially, originates from the precursors of those cells. The present data lend support to the view that maturation signals to gamma delta lineage-committed precursors can be delivered via TCR alpha beta heterodimers. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Maturation, Precursor, Transgenic mouse, TCR
in
European Journal of Immunology
volume
28
issue
3
pages
828 - 837
publisher
John Wiley & Sons
external identifiers
  • pmid:9541577
  • scopus:0031929363
ISSN
1521-4141
DOI
10.1002/(SICI)1521-4141(199803)28:03<828::AID-IMMU828>3.0.CO;2-X
language
English
LU publication?
yes
id
5d891dba-f812-4a05-9a21-f89c58143beb (old id 1113291)
date added to LUP
2008-07-14 16:10:41
date last changed
2017-09-24 03:35:52
@article{5d891dba-f812-4a05-9a21-f89c58143beb,
  abstract     = {The proportion of CD4- CD8- double-negative (DN) alpha beta T cells is increased both in the thymus and in peripheral lymphoid organs of TCR alpha chain-transgenic mice. In this report we have characterized this T cell population to elucidate its relationship to alpha beta and gamma delta T cells. We show that the transgenic DN cells are phenotypically similar to gamma delta T cells but distinct from DN NK T cells. The precursors of DN cells have neither rearranged endogenous TCR alpha genes nor been negatively selected by the MIsa antigen, suggesting that they originate from a differentiation stage before the onset of TCR alpha chain rearrangements and CD4/CD8 gene expression. Neither in-frame V delta D delta J delta nor V gamma J gamma rearrangements are over-represented in this population. However, since peripheral gamma delta T cells with functional TCR beta gene rearrangements have been depleted in the transgenics, we propose that the transgenic DN population, at least partially, originates from the precursors of those cells. The present data lend support to the view that maturation signals to gamma delta lineage-committed precursors can be delivered via TCR alpha beta heterodimers.},
  author       = {Fritsch, Maria and Andersson, Åsa and Leandersson, Karin and Ivars, Fredrik},
  issn         = {1521-4141},
  keyword      = {Maturation,Precursor,Transgenic mouse,TCR},
  language     = {eng},
  number       = {3},
  pages        = {828--837},
  publisher    = {John Wiley & Sons},
  series       = {European Journal of Immunology},
  title        = {A TCR alpha chain transgene induces maturation of CD4- CD8- alpha beta+ T cells from gamma delta T cell precursors},
  url          = {http://dx.doi.org/10.1002/(SICI)1521-4141(199803)28:03<828::AID-IMMU828>3.0.CO;2-X},
  volume       = {28},
  year         = {1998},
}