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Disease-related variations of the glycosylation of haptoglobin in the dog

Andersson, M; Stenström, Martin LU ; Vatne, M; Sevelius, E and Jonsson, L (1998) In Journal of Comparative Pathology 119(3). p.227-238
Abstract
Haptoglobin phenotypes have been shown in human medicine to be related to the prevalence of various diseases. Furthermore, abnormal glycosylation of haptoglobin has been reported as a consequence of liver disease, cancer and immunological disorders in man. To our knowledge, similar findings have not, so far, been reported in canine disease. The present paper describes a method for investigation of canine haptoglobin phenotypes and of microheterogeneity caused by altered glycosylation. The method consisted of isoelectric focusing (IEF) of dog serum, followed by immunoblotting. The results indicated the existence of only one canine haptoglobin phenotype with a characteristic microheterogeneity pattern in healthy dogs. Changes in this pattern... (More)
Haptoglobin phenotypes have been shown in human medicine to be related to the prevalence of various diseases. Furthermore, abnormal glycosylation of haptoglobin has been reported as a consequence of liver disease, cancer and immunological disorders in man. To our knowledge, similar findings have not, so far, been reported in canine disease. The present paper describes a method for investigation of canine haptoglobin phenotypes and of microheterogeneity caused by altered glycosylation. The method consisted of isoelectric focusing (IEF) of dog serum, followed by immunoblotting. The results indicated the existence of only one canine haptoglobin phenotype with a characteristic microheterogeneity pattern in healthy dogs. Changes in this pattern were found in serum from dogs with liver disease, predominantly chronic progressive hepatitis, and with different kinds of anaemia. Pretreatment of serum with neuraminidase or glycopeptidase F (PNGase F) resulted in identical IEF patterns of haptoglobin from healthy and diseased dogs. Moreover, a fucose-specific lectin was capable of binding to some of the abnormal haptoglobin fractions, mainly those found in association with anaemia. The changes described were interpreted as alterations of the carbohydrate content, with or without fucosylation, of some haptoglobin fractions. (Less)
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Contribution to journal
publication status
published
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in
Journal of Comparative Pathology
volume
119
issue
3
pages
227 - 238
publisher
Elsevier
external identifiers
  • pmid:9807725
  • scopus:0031728896
ISSN
1532-3129
language
English
LU publication?
yes
id
d7a9de57-0332-41df-867f-00829f36a6d0 (old id 1113311)
date added to LUP
2008-07-14 16:17:17
date last changed
2017-01-01 05:17:34
@article{d7a9de57-0332-41df-867f-00829f36a6d0,
  abstract     = {Haptoglobin phenotypes have been shown in human medicine to be related to the prevalence of various diseases. Furthermore, abnormal glycosylation of haptoglobin has been reported as a consequence of liver disease, cancer and immunological disorders in man. To our knowledge, similar findings have not, so far, been reported in canine disease. The present paper describes a method for investigation of canine haptoglobin phenotypes and of microheterogeneity caused by altered glycosylation. The method consisted of isoelectric focusing (IEF) of dog serum, followed by immunoblotting. The results indicated the existence of only one canine haptoglobin phenotype with a characteristic microheterogeneity pattern in healthy dogs. Changes in this pattern were found in serum from dogs with liver disease, predominantly chronic progressive hepatitis, and with different kinds of anaemia. Pretreatment of serum with neuraminidase or glycopeptidase F (PNGase F) resulted in identical IEF patterns of haptoglobin from healthy and diseased dogs. Moreover, a fucose-specific lectin was capable of binding to some of the abnormal haptoglobin fractions, mainly those found in association with anaemia. The changes described were interpreted as alterations of the carbohydrate content, with or without fucosylation, of some haptoglobin fractions.},
  author       = {Andersson, M and Stenström, Martin and Vatne, M and Sevelius, E and Jonsson, L},
  issn         = {1532-3129},
  language     = {eng},
  number       = {3},
  pages        = {227--238},
  publisher    = {Elsevier},
  series       = {Journal of Comparative Pathology},
  title        = {Disease-related variations of the glycosylation of haptoglobin in the dog},
  volume       = {119},
  year         = {1998},
}