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Clinical utility of immunoglobulin heavy chain gene rearrangement identification for tumour cell detection in multiple myeloma

Swedin, Agneta; Lenhoff, Stig; Olofsson, Tor LU ; Thuresson, Britt LU and Westin, Jan LU (1998) In British Journal of Haematology 103(4). p.1145-1151
Abstract
In an attempt to define the clinical utility of immunoglobulin heavy chain (IgH) gene rearrangement identification for tumour cell detection in multiple myeloma, we investigated 36 consecutive newly diagnosed patients intended for high-dose chemotherapy in a study protocol. After identification of the IgH rearrangement, an allele specific oligonucleotide (ASO) was constructed and used in a semiquantative PCR for minimal residual disease (MRD) evaluation. The myeloma-specific IgH gene rearrangement could be identified and an ASO primer constructed in 24 (67%) of the patients. All of these patients underwent transplantation; 22 were autologous, of whom three had PCR-negative stem cell harvests, and two were allogeneic. 10 patients achieved a... (More)
In an attempt to define the clinical utility of immunoglobulin heavy chain (IgH) gene rearrangement identification for tumour cell detection in multiple myeloma, we investigated 36 consecutive newly diagnosed patients intended for high-dose chemotherapy in a study protocol. After identification of the IgH rearrangement, an allele specific oligonucleotide (ASO) was constructed and used in a semiquantative PCR for minimal residual disease (MRD) evaluation. The myeloma-specific IgH gene rearrangement could be identified and an ASO primer constructed in 24 (67%) of the patients. All of these patients underwent transplantation; 22 were autologous, of whom three had PCR-negative stem cell harvests, and two were allogeneic. 10 patients achieved a clinical complete response (CR) and five were PCR negative in sequential bone marrow analyses. In patients not achieving CR, PCR negativity was occasionally found, but in general the PCR results reflected the clinical status of the patients. No consistent relationship between the bone marrow MRD status and the clinical course was found, and early relapses occurred also in PCR-negative patients. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
IgH, ASO-PCR, myeloma, MRD
in
British Journal of Haematology
volume
103
issue
4
pages
1145 - 1151
publisher
Federation of European Neuroscience Societies and Blackwell Publishing Ltd
external identifiers
  • pmid:9886333
  • scopus:0032411236
ISSN
0007-1048
DOI
10.1046/j.1365-2141.1998.01075.x
language
English
LU publication?
yes
id
894ff018-2580-42c7-aa62-589dea140bc7 (old id 1113580)
date added to LUP
2008-07-15 12:50:11
date last changed
2017-01-01 04:59:17
@article{894ff018-2580-42c7-aa62-589dea140bc7,
  abstract     = {In an attempt to define the clinical utility of immunoglobulin heavy chain (IgH) gene rearrangement identification for tumour cell detection in multiple myeloma, we investigated 36 consecutive newly diagnosed patients intended for high-dose chemotherapy in a study protocol. After identification of the IgH rearrangement, an allele specific oligonucleotide (ASO) was constructed and used in a semiquantative PCR for minimal residual disease (MRD) evaluation. The myeloma-specific IgH gene rearrangement could be identified and an ASO primer constructed in 24 (67%) of the patients. All of these patients underwent transplantation; 22 were autologous, of whom three had PCR-negative stem cell harvests, and two were allogeneic. 10 patients achieved a clinical complete response (CR) and five were PCR negative in sequential bone marrow analyses. In patients not achieving CR, PCR negativity was occasionally found, but in general the PCR results reflected the clinical status of the patients. No consistent relationship between the bone marrow MRD status and the clinical course was found, and early relapses occurred also in PCR-negative patients.},
  author       = {Swedin, Agneta and Lenhoff, Stig and Olofsson, Tor and Thuresson, Britt and Westin, Jan},
  issn         = {0007-1048},
  keyword      = {IgH,ASO-PCR,myeloma,MRD},
  language     = {eng},
  number       = {4},
  pages        = {1145--1151},
  publisher    = {Federation of European Neuroscience Societies and Blackwell Publishing Ltd},
  series       = {British Journal of Haematology},
  title        = {Clinical utility of immunoglobulin heavy chain gene rearrangement identification for tumour cell detection in multiple myeloma},
  url          = {http://dx.doi.org/10.1046/j.1365-2141.1998.01075.x},
  volume       = {103},
  year         = {1998},
}