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Genomic typing of the Kidd blood group locus by a single-tube allele-specific primer PCR technique

Irshaid, N M; Thuresson, Britt LU and Olsson, Martin L LU (1998) In British Journal of Haematology 102(4). p.1010-1014
Abstract
The Kidd (JK) blood group system is clinically important in transfusion medicine. Alloantibodies to antigens in this system may be produced following blood transfusion or during pregnancy and can result in serious haemolytic transfusion reactions and haemolytic disease of the newborn (HDN). JK antigens on erythrocytes are carried by glycoproteins with the capacity to transport urea through cell membranes. cDNA complementary to mRNA transcribed at the JK locus was cloned in 1994. The molecular basis of the Jk(a)/Jk(b) blood group polymorphism was recently shown to be a single nucleotide substitution predicting an amino acid change (Asp280Asn) in an extracellular loop of the JK glycoprotein. After confirmation of the JK gene polymorphism we... (More)
The Kidd (JK) blood group system is clinically important in transfusion medicine. Alloantibodies to antigens in this system may be produced following blood transfusion or during pregnancy and can result in serious haemolytic transfusion reactions and haemolytic disease of the newborn (HDN). JK antigens on erythrocytes are carried by glycoproteins with the capacity to transport urea through cell membranes. cDNA complementary to mRNA transcribed at the JK locus was cloned in 1994. The molecular basis of the Jk(a)/Jk(b) blood group polymorphism was recently shown to be a single nucleotide substitution predicting an amino acid change (Asp280Asn) in an extracellular loop of the JK glycoprotein. After confirmation of the JK gene polymorphism we developed a rapid and robust technique for JK genotyping with allele-specific primers in a single-tube PCR. In addition, a 217 bp intron located at nucleotides 811-812 in the JK gene was found and sequenced. The genotyping test was validated with samples from 106 Caucasian Swedish and 13 Black South African random blood donors. Complete phenotype-genotype correlations were obtained. However, four Jk(a-b-) samples of Polynesian and Finnish origin typed as Jk(b)Jk(b). Potential use of the presented method can be predicted in clinical transfusion medicine including prenatal determination of the JK genotype in a fetus at risk for HDN caused by JK antibodies. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Kidd blood group, allele, haemolytic disease of the newborn, genotyping, polymerase chain reaction
in
British Journal of Haematology
volume
102
issue
4
pages
1010 - 1014
publisher
Federation of European Neuroscience Societies and Blackwell Publishing Ltd
external identifiers
  • pmid:9734652
  • scopus:0031710591
ISSN
0007-1048
DOI
10.1046/j.1365-2141.1998.00874.x
language
English
LU publication?
yes
id
fb496fc9-8136-4d48-a256-455ac95c1941 (old id 1113691)
date added to LUP
2008-07-16 09:56:09
date last changed
2017-07-09 03:43:28
@article{fb496fc9-8136-4d48-a256-455ac95c1941,
  abstract     = {The Kidd (JK) blood group system is clinically important in transfusion medicine. Alloantibodies to antigens in this system may be produced following blood transfusion or during pregnancy and can result in serious haemolytic transfusion reactions and haemolytic disease of the newborn (HDN). JK antigens on erythrocytes are carried by glycoproteins with the capacity to transport urea through cell membranes. cDNA complementary to mRNA transcribed at the JK locus was cloned in 1994. The molecular basis of the Jk(a)/Jk(b) blood group polymorphism was recently shown to be a single nucleotide substitution predicting an amino acid change (Asp280Asn) in an extracellular loop of the JK glycoprotein. After confirmation of the JK gene polymorphism we developed a rapid and robust technique for JK genotyping with allele-specific primers in a single-tube PCR. In addition, a 217 bp intron located at nucleotides 811-812 in the JK gene was found and sequenced. The genotyping test was validated with samples from 106 Caucasian Swedish and 13 Black South African random blood donors. Complete phenotype-genotype correlations were obtained. However, four Jk(a-b-) samples of Polynesian and Finnish origin typed as Jk(b)Jk(b). Potential use of the presented method can be predicted in clinical transfusion medicine including prenatal determination of the JK genotype in a fetus at risk for HDN caused by JK antibodies.},
  author       = {Irshaid, N M and Thuresson, Britt and Olsson, Martin L},
  issn         = {0007-1048},
  keyword      = {Kidd blood group,allele,haemolytic disease of the newborn,genotyping,polymerase chain reaction},
  language     = {eng},
  number       = {4},
  pages        = {1010--1014},
  publisher    = {Federation of European Neuroscience Societies and Blackwell Publishing Ltd},
  series       = {British Journal of Haematology},
  title        = {Genomic typing of the Kidd blood group locus by a single-tube allele-specific primer PCR technique},
  url          = {http://dx.doi.org/10.1046/j.1365-2141.1998.00874.x},
  volume       = {102},
  year         = {1998},
}