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Physiological characterization of mBSA antigen induced arthritis in the rat. I. Vascular leakiness and pannus growth

Andersson, Sven LU ; Lexmuller, K and Ekstrom, G M (1998) In Journal of Rheumatology 25(9). p.1772-1777
Abstract
OBJECTIVE: To study the temporal relation between vascular inflammatory activity and synovial hyperplasia during the development of methylated bovine serum albumin (mBSA) antigen induced arthritis (AIA) in the rat, and to correlate these variables to changes in knee diameter. The influence of a single dose of indomethacin and methotrexate (MTX) on these measures was also determined. METHODS: Vascular inflammatory activity was assessed as extravasation of radiolabelled albumin. Synovial hyperplasia was followed by measurements of the increases in wet and dry weight of the anterior part of the periarticular soft tissue and by routine histology. RESULTS: The vascular inflammation peaked on Day 3 after antigen challenge. The pannus weight... (More)
OBJECTIVE: To study the temporal relation between vascular inflammatory activity and synovial hyperplasia during the development of methylated bovine serum albumin (mBSA) antigen induced arthritis (AIA) in the rat, and to correlate these variables to changes in knee diameter. The influence of a single dose of indomethacin and methotrexate (MTX) on these measures was also determined. METHODS: Vascular inflammatory activity was assessed as extravasation of radiolabelled albumin. Synovial hyperplasia was followed by measurements of the increases in wet and dry weight of the anterior part of the periarticular soft tissue and by routine histology. RESULTS: The vascular inflammation peaked on Day 3 after antigen challenge. The pannus weight increased at a slower pace, peaking on Day 7. No major difference between the sexes was found in these responses. Both variables were attenuated by MTX or indomethacin, suggesting a dependence between them. The water content of the pannus increased in tandem with the tissue growth but did not correlate to vascular leakiness, and is thus explained by the structural properties of the pannus rather than by the formation of inflammatory edema. In histological sections, ingrowth of pannus and destruction of cartilage was visible from Day 3 until the end of the experiment. CONCLUSION: Proliferative response follows the inflammatory vascular inflammation over time. The knee diameter, which is the most commonly used clinical measurement, seems mainly to be a reflection of the former variable. The effects of MTX and indomethacin suggest that the pannus formation is induced by the inflammatory activity in this model. (Less)
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author
organization
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Contribution to journal
publication status
published
subject
in
Journal of Rheumatology
volume
25
issue
9
pages
1772 - 1777
publisher
J Rheumatol Publ Co
external identifiers
  • pmid:9733459
  • scopus:0031787462
ISSN
0315-162X
language
English
LU publication?
yes
id
4f877fdb-cedf-488e-955e-30210ebecca7 (old id 1114007)
date added to LUP
2008-07-16 14:59:03
date last changed
2017-01-01 04:24:16
@article{4f877fdb-cedf-488e-955e-30210ebecca7,
  abstract     = {OBJECTIVE: To study the temporal relation between vascular inflammatory activity and synovial hyperplasia during the development of methylated bovine serum albumin (mBSA) antigen induced arthritis (AIA) in the rat, and to correlate these variables to changes in knee diameter. The influence of a single dose of indomethacin and methotrexate (MTX) on these measures was also determined. METHODS: Vascular inflammatory activity was assessed as extravasation of radiolabelled albumin. Synovial hyperplasia was followed by measurements of the increases in wet and dry weight of the anterior part of the periarticular soft tissue and by routine histology. RESULTS: The vascular inflammation peaked on Day 3 after antigen challenge. The pannus weight increased at a slower pace, peaking on Day 7. No major difference between the sexes was found in these responses. Both variables were attenuated by MTX or indomethacin, suggesting a dependence between them. The water content of the pannus increased in tandem with the tissue growth but did not correlate to vascular leakiness, and is thus explained by the structural properties of the pannus rather than by the formation of inflammatory edema. In histological sections, ingrowth of pannus and destruction of cartilage was visible from Day 3 until the end of the experiment. CONCLUSION: Proliferative response follows the inflammatory vascular inflammation over time. The knee diameter, which is the most commonly used clinical measurement, seems mainly to be a reflection of the former variable. The effects of MTX and indomethacin suggest that the pannus formation is induced by the inflammatory activity in this model.},
  author       = {Andersson, Sven and Lexmuller, K and Ekstrom, G M},
  issn         = {0315-162X},
  language     = {eng},
  number       = {9},
  pages        = {1772--1777},
  publisher    = {J Rheumatol Publ Co},
  series       = {Journal of Rheumatology},
  title        = {Physiological characterization of mBSA antigen induced arthritis in the rat. I. Vascular leakiness and pannus growth},
  volume       = {25},
  year         = {1998},
}