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Mucosal T lymphocyte numbers are selectively reduced in integrin alpha E (CD103)-deficient mice

Schon, M P; Arya, A; Murphy, E A; Adams, C M; Strauch, U G; Agace, William LU ; Marsal, J; Donohue, J P; Her, H and Beier, D R, et al. (1999) In Journal of Immunology 162(11). p.6641-6649
Abstract
The mucosal lymphocyte integrin alpha E(CD103)beta 7 is thought to be important for intraepithelial lymphocyte (IEL) localization or function. We cloned the murine integrin gene encoding alpha E, localized it to chromosome 11, and generated integrin alpha E-deficient mice. In alpha E-/- mice, intestinal and vaginal IEL numbers were reduced, consistent with the known binding of alpha E beta 7 to E-cadherin expressed on epithelial cells. However, it was surprising that lamina propria T lymphocyte numbers were diminished, as E-cadherin is not expressed in the lamina propria. In contrast, peribronchial, intrapulmonary, Peyer's patch, and splenic T lymphocyte numbers were not reduced in alpha E-deficient mice. Thus, alpha E beta 7 was important... (More)
The mucosal lymphocyte integrin alpha E(CD103)beta 7 is thought to be important for intraepithelial lymphocyte (IEL) localization or function. We cloned the murine integrin gene encoding alpha E, localized it to chromosome 11, and generated integrin alpha E-deficient mice. In alpha E-/- mice, intestinal and vaginal IEL numbers were reduced, consistent with the known binding of alpha E beta 7 to E-cadherin expressed on epithelial cells. However, it was surprising that lamina propria T lymphocyte numbers were diminished, as E-cadherin is not expressed in the lamina propria. In contrast, peribronchial, intrapulmonary, Peyer's patch, and splenic T lymphocyte numbers were not reduced in alpha E-deficient mice. Thus, alpha E beta 7 was important for generating or maintaining the gut and vaginal T lymphocytes located diffusely within the epithelium or lamina propria but not for generating the gut-associated organized lymphoid tissues. Finally, the impact of alpha E deficiency upon intestinal IEL numbers was greater at 3-4 wk of life than in younger animals, and affected the TCR alpha beta+ CD8+ T cells more than the gamma delta T cells or the TCR alpha beta+ CD4+CD8- population. These findings suggest that alpha E beta 7 is involved in the expansion/recruitment of TCR alpha beta+ CD8+ IEL following microbial colonization. Integrin alpha E-deficient mice will provide an important tool for studying the role of alpha E beta 7 and of alpha E beta 7-expressing mucosal T lymphocytes in vivo. (Less)
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Journal of Immunology
volume
162
issue
11
pages
6641 - 6649
publisher
American Association of Immunologists
external identifiers
  • pmid:10352281
ISSN
1550-6606
language
English
LU publication?
yes
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c33adcca-97f4-4a08-bdba-9710976954e4 (old id 1114203)
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http://www.jimmunol.org/cgi/content/full/162/11/6641
date added to LUP
2008-07-03 14:46:17
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2016-04-16 04:18:43
@article{c33adcca-97f4-4a08-bdba-9710976954e4,
  abstract     = {The mucosal lymphocyte integrin alpha E(CD103)beta 7 is thought to be important for intraepithelial lymphocyte (IEL) localization or function. We cloned the murine integrin gene encoding alpha E, localized it to chromosome 11, and generated integrin alpha E-deficient mice. In alpha E-/- mice, intestinal and vaginal IEL numbers were reduced, consistent with the known binding of alpha E beta 7 to E-cadherin expressed on epithelial cells. However, it was surprising that lamina propria T lymphocyte numbers were diminished, as E-cadherin is not expressed in the lamina propria. In contrast, peribronchial, intrapulmonary, Peyer's patch, and splenic T lymphocyte numbers were not reduced in alpha E-deficient mice. Thus, alpha E beta 7 was important for generating or maintaining the gut and vaginal T lymphocytes located diffusely within the epithelium or lamina propria but not for generating the gut-associated organized lymphoid tissues. Finally, the impact of alpha E deficiency upon intestinal IEL numbers was greater at 3-4 wk of life than in younger animals, and affected the TCR alpha beta+ CD8+ T cells more than the gamma delta T cells or the TCR alpha beta+ CD4+CD8- population. These findings suggest that alpha E beta 7 is involved in the expansion/recruitment of TCR alpha beta+ CD8+ IEL following microbial colonization. Integrin alpha E-deficient mice will provide an important tool for studying the role of alpha E beta 7 and of alpha E beta 7-expressing mucosal T lymphocytes in vivo.},
  author       = {Schon, M P and Arya, A and Murphy, E A and Adams, C M and Strauch, U G and Agace, William and Marsal, J and Donohue, J P and Her, H and Beier, D R and Olson, S and Lefrancois, L and Brenner, M B and Grusby, M J and Parker, C M},
  issn         = {1550-6606},
  language     = {eng},
  number       = {11},
  pages        = {6641--6649},
  publisher    = {American Association of Immunologists},
  series       = {Journal of Immunology},
  title        = {Mucosal T lymphocyte numbers are selectively reduced in integrin alpha E (CD103)-deficient mice},
  volume       = {162},
  year         = {1999},
}