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Energy transfer between fluorescein isothiocyanate and propidium iodide--a problem in the estimation of Tpot with the bromodeoxyuridine-DNA flow cytometry technique?

Johansson, Maria C LU ; Baldetorp, Bo LU and Oredsson, Stina LU (1999) In Analytical Cellular Pathology 19(2). p.91-98
Abstract
Energy transfer in flow cytometry can occur when two fluorochromes are bound in close proximity (generally within 100 A) and the emission spectrum of one fluorochrome overlaps significantly with the excitation spectrum of the other. The latter criterium is fulfilled for the fluorochromes fluorescein isothiocyanate and propidium iodide and also the former when they, e.g., are used in bromodeoxyuridine - DNA flow cytometry methods. In the present growth kinetic study using this method, we show that energy transfer does take place between fluorescein isothiocyanate and propidium iodide which results in a detected increase in DNA content with 2-3%. Despite the erroneous increase in the obtained DNA content values, this does not seem to have... (More)
Energy transfer in flow cytometry can occur when two fluorochromes are bound in close proximity (generally within 100 A) and the emission spectrum of one fluorochrome overlaps significantly with the excitation spectrum of the other. The latter criterium is fulfilled for the fluorochromes fluorescein isothiocyanate and propidium iodide and also the former when they, e.g., are used in bromodeoxyuridine - DNA flow cytometry methods. In the present growth kinetic study using this method, we show that energy transfer does take place between fluorescein isothiocyanate and propidium iodide which results in a detected increase in DNA content with 2-3%. Despite the erroneous increase in the obtained DNA content values, this does not seem to have any influence on the calculation of DNA synthesis time and potential doubling time where the DNA content, based on the relative movement principle of the labelled cells, is used. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Energy transfer
in
Analytical Cellular Pathology
volume
19
issue
2
pages
91 - 98
publisher
IOS Press
external identifiers
  • pmid:10746439
ISSN
0921-8912
language
English
LU publication?
yes
id
59dcaf44-78fe-4793-ba90-a236e8a0335b (old id 1114347)
alternative location
http://iospress.metapress.com/content/ubqbc1hc6gxqwauy/fulltext.pdf
date added to LUP
2008-07-03 16:56:54
date last changed
2016-04-16 04:55:23
@article{59dcaf44-78fe-4793-ba90-a236e8a0335b,
  abstract     = {Energy transfer in flow cytometry can occur when two fluorochromes are bound in close proximity (generally within 100 A) and the emission spectrum of one fluorochrome overlaps significantly with the excitation spectrum of the other. The latter criterium is fulfilled for the fluorochromes fluorescein isothiocyanate and propidium iodide and also the former when they, e.g., are used in bromodeoxyuridine - DNA flow cytometry methods. In the present growth kinetic study using this method, we show that energy transfer does take place between fluorescein isothiocyanate and propidium iodide which results in a detected increase in DNA content with 2-3%. Despite the erroneous increase in the obtained DNA content values, this does not seem to have any influence on the calculation of DNA synthesis time and potential doubling time where the DNA content, based on the relative movement principle of the labelled cells, is used.},
  author       = {Johansson, Maria C and Baldetorp, Bo and Oredsson, Stina},
  issn         = {0921-8912},
  keyword      = {Energy transfer},
  language     = {eng},
  number       = {2},
  pages        = {91--98},
  publisher    = {IOS Press},
  series       = {Analytical Cellular Pathology},
  title        = {Energy transfer between fluorescein isothiocyanate and propidium iodide--a problem in the estimation of Tpot with the bromodeoxyuridine-DNA flow cytometry technique?},
  volume       = {19},
  year         = {1999},
}