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Chromosomal aberrations in breast cancer: a comparison between cytogenetics and comparative genomic hybridization

Persson, Karin; Pandis, Nikos; Mertens, Fredrik LU ; Borg, Åke LU ; Baldetorp, Bo LU ; Killander, Dick LU and Isola, Jorma LU (1999) In Genes, Chromosomes and Cancer 25(2). p.115-122
Abstract
The analysis of chromosomal imbalances in solid tumors using comparative genetic hybridization (CGH) has gained much attention. A survey of the literature suggests that CGH is more sensitive in detecting copy number aberrations than is karyotyping, although careful comparisons between CGH and cytogenetics have not been performed. Here, we compared cytogenetics and CGH in 29 invasive breast cancers after converting the karyotypes into net copy number gains and losses. We found 15 tumors (56%) with a significant agreement between the two methods and 12 tumors (44%) where the methods were in disagreement (two cases failed CGH analysis). Interestingly, in 13 of the 15 tumors where the two methods were concordant, there was also a strong... (More)
The analysis of chromosomal imbalances in solid tumors using comparative genetic hybridization (CGH) has gained much attention. A survey of the literature suggests that CGH is more sensitive in detecting copy number aberrations than is karyotyping, although careful comparisons between CGH and cytogenetics have not been performed. Here, we compared cytogenetics and CGH in 29 invasive breast cancers after converting the karyotypes into net copy number gains and losses. We found 15 tumors (56%) with a significant agreement between the two methods and 12 tumors (44%) where the methods were in disagreement (two cases failed CGH analysis). Interestingly, in 13 of the 15 tumors where the two methods were concordant, there was also a strong correlation between chromosome index and DNA index by flow cytometry. In the opposite situation, i.e., when chromosome and DNA indices were not matching, there was disagreement between cytogenetics and CGH in 10 of the 12 tumors. Of the discordant cases, all except one had a "simple" abnormal karyotype. Unresolved chromosomal aberrations (marker chromosomes, homogeneously staining regions, double minutes) could not completely explain the differences between CGH and karyotyping. A likely explanation for the discrepancies is that the methods analyzed different cell populations. Gains and losses found by CGH represented the predominant (often aneuploid) clone, whereas the abnormal, near-diploid karyotypes represented minor cell clone(s), which, for unknown reasons, had a growth advantage in vitro. (Less)
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author
organization
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Contribution to journal
publication status
published
subject
in
Genes, Chromosomes and Cancer
volume
25
issue
2
pages
115 - 122
publisher
John Wiley & Sons
external identifiers
  • pmid:10337995
  • scopus:0032940286
ISSN
1045-2257
language
English
LU publication?
yes
id
ca795b15-d585-4386-9938-9396fb89d642 (old id 1114350)
alternative location
http://www3.interscience.wiley.com/cgi-bin/fulltext/61006474/PDFSTART
date added to LUP
2008-07-04 08:30:13
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2017-04-09 03:33:49
@article{ca795b15-d585-4386-9938-9396fb89d642,
  abstract     = {The analysis of chromosomal imbalances in solid tumors using comparative genetic hybridization (CGH) has gained much attention. A survey of the literature suggests that CGH is more sensitive in detecting copy number aberrations than is karyotyping, although careful comparisons between CGH and cytogenetics have not been performed. Here, we compared cytogenetics and CGH in 29 invasive breast cancers after converting the karyotypes into net copy number gains and losses. We found 15 tumors (56%) with a significant agreement between the two methods and 12 tumors (44%) where the methods were in disagreement (two cases failed CGH analysis). Interestingly, in 13 of the 15 tumors where the two methods were concordant, there was also a strong correlation between chromosome index and DNA index by flow cytometry. In the opposite situation, i.e., when chromosome and DNA indices were not matching, there was disagreement between cytogenetics and CGH in 10 of the 12 tumors. Of the discordant cases, all except one had a "simple" abnormal karyotype. Unresolved chromosomal aberrations (marker chromosomes, homogeneously staining regions, double minutes) could not completely explain the differences between CGH and karyotyping. A likely explanation for the discrepancies is that the methods analyzed different cell populations. Gains and losses found by CGH represented the predominant (often aneuploid) clone, whereas the abnormal, near-diploid karyotypes represented minor cell clone(s), which, for unknown reasons, had a growth advantage in vitro.},
  author       = {Persson, Karin and Pandis, Nikos and Mertens, Fredrik and Borg, Åke and Baldetorp, Bo and Killander, Dick and Isola, Jorma},
  issn         = {1045-2257},
  language     = {eng},
  number       = {2},
  pages        = {115--122},
  publisher    = {John Wiley & Sons},
  series       = {Genes, Chromosomes and Cancer},
  title        = {Chromosomal aberrations in breast cancer: a comparison between cytogenetics and comparative genomic hybridization},
  volume       = {25},
  year         = {1999},
}