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Differential modulation of caffeine- and IP3-induced calcium release in cultured arterial tissue

Dreja, Karl LU and Hellstrand, Per LU (1999) In American Journal of Physiology 276(5). p.1115-1120
Abstract
To investigate the Ca2+-dependent plasticity of sarcoplasmic reticulum (SR) function in vascular smooth muscle, transient responses to agents releasing intracellular Ca2+ by either ryanodine (caffeine) or D-myo-inositol 1,4,5-trisphosphate [IP3; produced in response to norepinephrine (NE), 5-hydroxytryptamine (5-HT), arginine vasopressin (AVP)] receptors in rat tail arterial rings were evaluated after 4 days of organ culture. Force transients induced by all agents were increased compared with those induced in fresh rings. Stimulation by 10% FCS during culture further potentiated the force and Ca2+ responses to caffeine (20 mM) but not to NE (10 microM), 5-HT (10 microM), or AVP (0.1 microM). The effect was persistent, and SR capacity was... (More)
To investigate the Ca2+-dependent plasticity of sarcoplasmic reticulum (SR) function in vascular smooth muscle, transient responses to agents releasing intracellular Ca2+ by either ryanodine (caffeine) or D-myo-inositol 1,4,5-trisphosphate [IP3; produced in response to norepinephrine (NE), 5-hydroxytryptamine (5-HT), arginine vasopressin (AVP)] receptors in rat tail arterial rings were evaluated after 4 days of organ culture. Force transients induced by all agents were increased compared with those induced in fresh rings. Stimulation by 10% FCS during culture further potentiated the force and Ca2+ responses to caffeine (20 mM) but not to NE (10 microM), 5-HT (10 microM), or AVP (0.1 microM). The effect was persistent, and SR capacity was not altered after reversible depletion of stores with cyclopiazonic acid. The effects of serum could be mimicked by culture in depolarizing medium (30 mM K+) and blocked by the addition of verapamil (1 microM) or EGTA (1 mM) to the medium, lowering intracellular Ca2+ concentration ([Ca2+]i) during culture. These results show that modulation of SR function can occur in vitro by a mechanism dependent on long-term levels of basal [Ca2+]i and involving ryanodine- but not IP3 receptor-mediated Ca2+ release. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
sarcoplasmic reticulum, organ culture, tail artery, D-myo-inositol 1, 4, 5-trisphosphate
in
American Journal of Physiology
volume
276
issue
5
pages
1115 - 1120
publisher
American Pysiological Society
external identifiers
  • pmid:10329960
ISSN
0002-9513
language
English
LU publication?
yes
id
bff1db48-2211-4484-bcc6-cddfd1ab77eb (old id 1114548)
alternative location
http://ajpcell.physiology.org/cgi/content/full/276/5/C1115
date added to LUP
2008-07-04 12:37:18
date last changed
2016-04-16 03:49:33
@article{bff1db48-2211-4484-bcc6-cddfd1ab77eb,
  abstract     = {To investigate the Ca2+-dependent plasticity of sarcoplasmic reticulum (SR) function in vascular smooth muscle, transient responses to agents releasing intracellular Ca2+ by either ryanodine (caffeine) or D-myo-inositol 1,4,5-trisphosphate [IP3; produced in response to norepinephrine (NE), 5-hydroxytryptamine (5-HT), arginine vasopressin (AVP)] receptors in rat tail arterial rings were evaluated after 4 days of organ culture. Force transients induced by all agents were increased compared with those induced in fresh rings. Stimulation by 10% FCS during culture further potentiated the force and Ca2+ responses to caffeine (20 mM) but not to NE (10 microM), 5-HT (10 microM), or AVP (0.1 microM). The effect was persistent, and SR capacity was not altered after reversible depletion of stores with cyclopiazonic acid. The effects of serum could be mimicked by culture in depolarizing medium (30 mM K+) and blocked by the addition of verapamil (1 microM) or EGTA (1 mM) to the medium, lowering intracellular Ca2+ concentration ([Ca2+]i) during culture. These results show that modulation of SR function can occur in vitro by a mechanism dependent on long-term levels of basal [Ca2+]i and involving ryanodine- but not IP3 receptor-mediated Ca2+ release.},
  author       = {Dreja, Karl and Hellstrand, Per},
  issn         = {0002-9513},
  keyword      = {sarcoplasmic reticulum,organ culture,tail artery,D-myo-inositol 1,4,5-trisphosphate},
  language     = {eng},
  number       = {5},
  pages        = {1115--1120},
  publisher    = {American Pysiological Society},
  series       = {American Journal of Physiology},
  title        = {Differential modulation of caffeine- and IP3-induced calcium release in cultured arterial tissue},
  volume       = {276},
  year         = {1999},
}