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Neutrophil recruitment and resistance to urinary tract infection

Haraoka, Masashi; Hang, Long; Frendeus, Björn; Godaly, Gabriela LU ; Burdick, M; Strieter, R and Svanborg, Catharina LU (1999) In Journal of Infectious Diseases 180(4). p.1220-1229
Abstract
This study examined the role of neutrophil leukocytes for the antibacterial defense at mucosal infection sites. Urinary tract infection (UTI) was established by injection into the bladder lumen of Escherichia coli 1177, a fully virulent clinical isolate. Infection of C3H/HeN (lpsn, lpsn) mice recruited neutrophils into the urinary tract, and bacteria were cleared from kidneys and bladders. The neutrophil response was absent in C3H/HeJ (lpsd, lpsd) mice, and bacteria persisted in the tissues. Peripheral neutrophil depletion of C3H/HeN mice was subsequently achieved by pretreatment with the granulocyte-specific antibody RB6-8C5. The E. coli-induced neutrophil recruitment was inhibited, as shown by immunohistochemistry and tissue... (More)
This study examined the role of neutrophil leukocytes for the antibacterial defense at mucosal infection sites. Urinary tract infection (UTI) was established by injection into the bladder lumen of Escherichia coli 1177, a fully virulent clinical isolate. Infection of C3H/HeN (lpsn, lpsn) mice recruited neutrophils into the urinary tract, and bacteria were cleared from kidneys and bladders. The neutrophil response was absent in C3H/HeJ (lpsd, lpsd) mice, and bacteria persisted in the tissues. Peripheral neutrophil depletion of C3H/HeN mice was subsequently achieved by pretreatment with the granulocyte-specific antibody RB6-8C5. The E. coli-induced neutrophil recruitment was inhibited, as shown by immunohistochemistry and tissue myeloperoxidase quantitation. As a consequence, bacterial clearance from kidneys and bladders was drastically impaired. Antibody treatment of C3H/HeJ mice had only a marginal effect. The results show that neutrophils are essential for bacterial clearance from the urinary tract and that the neutrophil recruitment deficiency in C3H/HeJ mice explains their susceptibility to gram-negative UTI. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Infectious Diseases
volume
180
issue
4
pages
1220 - 1229
publisher
Oxford University Press
external identifiers
  • pmid:10479151
  • scopus:0033389454
ISSN
1537-6613
DOI
10.1086/315006
language
English
LU publication?
yes
id
97fb1208-edaa-4300-b643-ae977b6771d4 (old id 1114733)
date added to LUP
2008-07-04 15:52:58
date last changed
2017-08-06 04:24:37
@article{97fb1208-edaa-4300-b643-ae977b6771d4,
  abstract     = {This study examined the role of neutrophil leukocytes for the antibacterial defense at mucosal infection sites. Urinary tract infection (UTI) was established by injection into the bladder lumen of Escherichia coli 1177, a fully virulent clinical isolate. Infection of C3H/HeN (lpsn, lpsn) mice recruited neutrophils into the urinary tract, and bacteria were cleared from kidneys and bladders. The neutrophil response was absent in C3H/HeJ (lpsd, lpsd) mice, and bacteria persisted in the tissues. Peripheral neutrophil depletion of C3H/HeN mice was subsequently achieved by pretreatment with the granulocyte-specific antibody RB6-8C5. The E. coli-induced neutrophil recruitment was inhibited, as shown by immunohistochemistry and tissue myeloperoxidase quantitation. As a consequence, bacterial clearance from kidneys and bladders was drastically impaired. Antibody treatment of C3H/HeJ mice had only a marginal effect. The results show that neutrophils are essential for bacterial clearance from the urinary tract and that the neutrophil recruitment deficiency in C3H/HeJ mice explains their susceptibility to gram-negative UTI.},
  author       = {Haraoka, Masashi and Hang, Long and Frendeus, Björn and Godaly, Gabriela and Burdick, M and Strieter, R and Svanborg, Catharina},
  issn         = {1537-6613},
  language     = {eng},
  number       = {4},
  pages        = {1220--1229},
  publisher    = {Oxford University Press},
  series       = {Journal of Infectious Diseases},
  title        = {Neutrophil recruitment and resistance to urinary tract infection},
  url          = {http://dx.doi.org/10.1086/315006},
  volume       = {180},
  year         = {1999},
}