Patterns of cell death and dopaminergic neuron survival in intrastriatal nigral grafts
(1999) In Experimental Neurology 160(1). p.88-279- Abstract
- Previous studies indicate that 80-95% of grafted dopamine neurons die following implantation of embryonic ventral mesencephalic tissue into the striatum. It is believed that the majority die within the first 1-3 weeks after surgery. The aim of this study was to study when and where the implanted neurons die, using the novel fluorescent stain Fluoro-Jade. Fluoro-Jade has recently been shown to stain cell bodies, dendrites, axons, and terminals of degenerating neurons. We transplanted dissociated ventral mesencephalic tissue from embryonic day 14 rat embryos into intact adult rat striatum. After perfusion and sectioning of the implanted rat brains, the number and distribution of Fluoro-Jade and tyrosine hydroxylase-positive neurons were... (More)
- Previous studies indicate that 80-95% of grafted dopamine neurons die following implantation of embryonic ventral mesencephalic tissue into the striatum. It is believed that the majority die within the first 1-3 weeks after surgery. The aim of this study was to study when and where the implanted neurons die, using the novel fluorescent stain Fluoro-Jade. Fluoro-Jade has recently been shown to stain cell bodies, dendrites, axons, and terminals of degenerating neurons. We transplanted dissociated ventral mesencephalic tissue from embryonic day 14 rat embryos into intact adult rat striatum. After perfusion and sectioning of the implanted rat brains, the number and distribution of Fluoro-Jade and tyrosine hydroxylase-positive neurons were evaluated at 6, 10, 14, and 42 days posttransplantation. Intensely Fluoro-Jade stained neurons were numerous in the grafts at 6 and 10 days after graft surgery; appeared in reduced numbers at 14 days; and had disappeared by the 42-day time point. The number of surviving tyrosine hydroxylase-positive, dopaminergic neurons in the grafts did not change between 6 and 42 days and the low survival rate confirmed that over 90% of these neurons had died during the first week. Assessment of the distribution of neurons positive for Fluoro-Jade or tyrosine hydroxylase revealed higher numbers of neurons stained for these markers located at the periphery than the center of the grafts, and this pattern did not change over time. This study indicates that transplanted neurons continue to die up to 14 days after grafting. Since the majority of transplanted tyrosine hydroxylase-positive neurons most probably die before 6 days after transplantation, neuroprotective strategies should primarily focus on the transplantation procedure and the first week after implantation. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1114740
- author
- Emgård-Mattson, Mia
LU
; Karlsson, Jenny
LU
; Hansson, Oskar
LU
and Brundin, Patrik LU
- organization
- publishing date
- 1999
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- dopamine, nigral grafts, Fluoro-Jade, cell death, Parkinson's disease, transplantation
- in
- Experimental Neurology
- volume
- 160
- issue
- 1
- pages
- 88 - 279
- publisher
- Elsevier
- external identifiers
-
- pmid:10630212
- scopus:0345102465
- ISSN
- 0014-4886
- DOI
- 10.1006/exnr.1999.7198
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Psychiatry/Primary Care/Public Health (013240500), Department of Experimental Medical Science (013210000)
- id
- b4c34b13-026f-4e2b-9c26-f31829bbf7fc (old id 1114740)
- date added to LUP
- 2016-04-01 11:38:54
- date last changed
- 2022-01-26 08:05:39
@article{b4c34b13-026f-4e2b-9c26-f31829bbf7fc, abstract = {{Previous studies indicate that 80-95% of grafted dopamine neurons die following implantation of embryonic ventral mesencephalic tissue into the striatum. It is believed that the majority die within the first 1-3 weeks after surgery. The aim of this study was to study when and where the implanted neurons die, using the novel fluorescent stain Fluoro-Jade. Fluoro-Jade has recently been shown to stain cell bodies, dendrites, axons, and terminals of degenerating neurons. We transplanted dissociated ventral mesencephalic tissue from embryonic day 14 rat embryos into intact adult rat striatum. After perfusion and sectioning of the implanted rat brains, the number and distribution of Fluoro-Jade and tyrosine hydroxylase-positive neurons were evaluated at 6, 10, 14, and 42 days posttransplantation. Intensely Fluoro-Jade stained neurons were numerous in the grafts at 6 and 10 days after graft surgery; appeared in reduced numbers at 14 days; and had disappeared by the 42-day time point. The number of surviving tyrosine hydroxylase-positive, dopaminergic neurons in the grafts did not change between 6 and 42 days and the low survival rate confirmed that over 90% of these neurons had died during the first week. Assessment of the distribution of neurons positive for Fluoro-Jade or tyrosine hydroxylase revealed higher numbers of neurons stained for these markers located at the periphery than the center of the grafts, and this pattern did not change over time. This study indicates that transplanted neurons continue to die up to 14 days after grafting. Since the majority of transplanted tyrosine hydroxylase-positive neurons most probably die before 6 days after transplantation, neuroprotective strategies should primarily focus on the transplantation procedure and the first week after implantation.}}, author = {{Emgård-Mattson, Mia and Karlsson, Jenny and Hansson, Oskar and Brundin, Patrik}}, issn = {{0014-4886}}, keywords = {{dopamine; nigral grafts; Fluoro-Jade; cell death; Parkinson's disease; transplantation}}, language = {{eng}}, number = {{1}}, pages = {{88--279}}, publisher = {{Elsevier}}, series = {{Experimental Neurology}}, title = {{Patterns of cell death and dopaminergic neuron survival in intrastriatal nigral grafts}}, url = {{http://dx.doi.org/10.1006/exnr.1999.7198}}, doi = {{10.1006/exnr.1999.7198}}, volume = {{160}}, year = {{1999}}, }