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Neuronal death in nigral grafts in the absence of poly (ADP-ribose) polymerase activation

Schierle, Gabriele LU ; Hansson, Oskar LU ; Ferrando-May, E; Nicotera, P; Brundin, Patrik LU and Leist, M (1999) In NeuroReport 10(16). p.3347-3351
Abstract
The exact causes of the extensive cell death in nigral transplants are still unknown. Since poly-(ADP-ribose) polymerase (PARP) overactivation has been implicated in neuronal death, we examined the effects of PARP on the survival of nigral grafts by using donor tissue from PARP knock-out or wild-type mice. Eight hours after preparation of the nigral cell suspension, cell damage was quantified by measurement of lactate dehydrogenase release, DNA fragmentation and caspase activation. At this stage, PARP deletion had no protective effect. Moreover, neither the survival of transplanted dopaminergic neurons, nor the functional recovery of hemiparkinsonian graft recipients were improved by the absence of PARP. We conclude that cell death in... (More)
The exact causes of the extensive cell death in nigral transplants are still unknown. Since poly-(ADP-ribose) polymerase (PARP) overactivation has been implicated in neuronal death, we examined the effects of PARP on the survival of nigral grafts by using donor tissue from PARP knock-out or wild-type mice. Eight hours after preparation of the nigral cell suspension, cell damage was quantified by measurement of lactate dehydrogenase release, DNA fragmentation and caspase activation. At this stage, PARP deletion had no protective effect. Moreover, neither the survival of transplanted dopaminergic neurons, nor the functional recovery of hemiparkinsonian graft recipients were improved by the absence of PARP. We conclude that cell death in embryonic nigral grafts is not affected by the absence of PARP activation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
NeuroReport
volume
10
issue
16
pages
3347 - 3351
publisher
Lippincott Williams & Wilkins
external identifiers
  • pmid:10599843
  • scopus:0032693326
ISSN
1473-558X
language
English
LU publication?
yes
id
7ba82877-120d-4fa3-ab0c-834871ac5408 (old id 1114754)
date added to LUP
2008-07-04 16:07:10
date last changed
2017-01-01 04:24:54
@article{7ba82877-120d-4fa3-ab0c-834871ac5408,
  abstract     = {The exact causes of the extensive cell death in nigral transplants are still unknown. Since poly-(ADP-ribose) polymerase (PARP) overactivation has been implicated in neuronal death, we examined the effects of PARP on the survival of nigral grafts by using donor tissue from PARP knock-out or wild-type mice. Eight hours after preparation of the nigral cell suspension, cell damage was quantified by measurement of lactate dehydrogenase release, DNA fragmentation and caspase activation. At this stage, PARP deletion had no protective effect. Moreover, neither the survival of transplanted dopaminergic neurons, nor the functional recovery of hemiparkinsonian graft recipients were improved by the absence of PARP. We conclude that cell death in embryonic nigral grafts is not affected by the absence of PARP activation.},
  author       = {Schierle, Gabriele and Hansson, Oskar and Ferrando-May, E and Nicotera, P and Brundin, Patrik and Leist, M},
  issn         = {1473-558X},
  language     = {eng},
  number       = {16},
  pages        = {3347--3351},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {NeuroReport},
  title        = {Neuronal death in nigral grafts in the absence of poly (ADP-ribose) polymerase activation},
  volume       = {10},
  year         = {1999},
}