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Decreased frequency of intracellular IFN-gamma producing T cells in whole blood preparations from patients with atopic dermatitis.

Källström, Eva; Roscher, Ingrid; Andreasson, Annica; Bäck, Ove LU and Van Hage-Hamsten, Marianne (2002) In Experimental Dermatology 11(6). p.556-563
Abstract
There have been contradictory reports on the shift in the T-cell cytokine expression pattern of peripheral blood mononuclear cells from patients with atopic dermatitis (AD); more specifically the interleukin (IL)-4 and interferon (IFN)-gamma profiles. The aim of this study was to shed further light on this contradiction by measuring the intracellular cytokines IL-4 and IFN-gamma by flow cytometry on unseparated whole blood to obtain results that, as accurately as possible, reflect the situation in circulating cells in vivo. The patient group including 64 patients with AD was compared with 18 nonatopic healthy adults. The results showed that the percentage of CD4+ T cells expressing IFN-gamma was significantly decreased (P <= 0.001), as... (More)
There have been contradictory reports on the shift in the T-cell cytokine expression pattern of peripheral blood mononuclear cells from patients with atopic dermatitis (AD); more specifically the interleukin (IL)-4 and interferon (IFN)-gamma profiles. The aim of this study was to shed further light on this contradiction by measuring the intracellular cytokines IL-4 and IFN-gamma by flow cytometry on unseparated whole blood to obtain results that, as accurately as possible, reflect the situation in circulating cells in vivo. The patient group including 64 patients with AD was compared with 18 nonatopic healthy adults. The results showed that the percentage of CD4+ T cells expressing IFN-gamma was significantly decreased (P <= 0.001), as well as the percentage expressing IL-4 (P < 0.05) in AD patients compared with healthy controls. Furthermore, in supernatants from whole blood samples stimulated with phorbol 12-myristate 13-acetate and ionomycin, production of IFN-gamma was significantly decreased, while IL-4 production remained unchanged in AD patients compared with healthy controls. We also investigated if there was a relationship between serum IgE level and Phadiatop®, a screening test for atopy, vs. the levels of IL-4 and IFN-gamma , but found no correlation with either. However, there was a significant correlation between disease severity and the level of total IgE (r = 0.67, P < 0.05). In conclusion, our results support the evidence for a decreased ability of peripheral CD4+ T cells to produce IFN-gamma among AD patients. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Experimental Dermatology
volume
11
issue
6
pages
556 - 563
publisher
Wiley-Blackwell
external identifiers
  • pmid:12473063
  • wos:000180092200008
  • scopus:0041510491
ISSN
0906-6705
DOI
10.1034/j.1600-0625.2002.110608.x
language
English
LU publication?
yes
id
b14e5db5-aadc-4c86-a6ed-36d54161a887 (old id 111483)
alternative location
http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=pubmed&list_uids=12473063
date added to LUP
2007-07-03 08:24:12
date last changed
2017-01-01 05:02:43
@article{b14e5db5-aadc-4c86-a6ed-36d54161a887,
  abstract     = {There have been contradictory reports on the shift in the T-cell cytokine expression pattern of peripheral blood mononuclear cells from patients with atopic dermatitis (AD); more specifically the interleukin (IL)-4 and interferon (IFN)-gamma profiles. The aim of this study was to shed further light on this contradiction by measuring the intracellular cytokines IL-4 and IFN-gamma by flow cytometry on unseparated whole blood to obtain results that, as accurately as possible, reflect the situation in circulating cells in vivo. The patient group including 64 patients with AD was compared with 18 nonatopic healthy adults. The results showed that the percentage of CD4+ T cells expressing IFN-gamma was significantly decreased (P &lt;= 0.001), as well as the percentage expressing IL-4 (P &lt; 0.05) in AD patients compared with healthy controls. Furthermore, in supernatants from whole blood samples stimulated with phorbol 12-myristate 13-acetate and ionomycin, production of IFN-gamma was significantly decreased, while IL-4 production remained unchanged in AD patients compared with healthy controls. We also investigated if there was a relationship between serum IgE level and Phadiatop®, a screening test for atopy, vs. the levels of IL-4 and IFN-gamma , but found no correlation with either. However, there was a significant correlation between disease severity and the level of total IgE (r = 0.67, P &lt; 0.05). In conclusion, our results support the evidence for a decreased ability of peripheral CD4+ T cells to produce IFN-gamma among AD patients.},
  author       = {Källström, Eva and Roscher, Ingrid and Andreasson, Annica and Bäck, Ove and Van Hage-Hamsten, Marianne},
  issn         = {0906-6705},
  language     = {eng},
  number       = {6},
  pages        = {556--563},
  publisher    = {Wiley-Blackwell},
  series       = {Experimental Dermatology},
  title        = {Decreased frequency of intracellular IFN-gamma producing T cells in whole blood preparations from patients with atopic dermatitis.},
  url          = {http://dx.doi.org/10.1034/j.1600-0625.2002.110608.x},
  volume       = {11},
  year         = {2002},
}