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Long-term effects of Ca(2+) on structure and contractility of vascular smooth muscle

Lindqvist, Anders LU ; Nordström, Ina LU ; Malmqvist, Ulf LU ; Nordenfelt, Patrik and Hellstrand, Per LU (1999) In American Journal of Physiology 277(1). p.64-73
Abstract
Culture of dispersed smooth muscle cells is known to cause rapid modulation from the contractile to the synthetic cellular phenotype. However, organ culture of smooth muscle tissue, with maintained extracellular matrix and cell-cell contacts, may facilitate maintenance of the contractile phenotype. To test the influence of culture conditions, structural, functional, and biochemical properties of rat tail arterial rings were investigated after culture. Rings were cultured for 4 days in the absence and presence of 10% FCS and then mounted for physiological experiments. Intracellular Ca(2+) concentration ([Ca(2+)](i)) after stimulation with norepinephrine was similar in rings cultured with and without FCS, whereas force development after FCS... (More)
Culture of dispersed smooth muscle cells is known to cause rapid modulation from the contractile to the synthetic cellular phenotype. However, organ culture of smooth muscle tissue, with maintained extracellular matrix and cell-cell contacts, may facilitate maintenance of the contractile phenotype. To test the influence of culture conditions, structural, functional, and biochemical properties of rat tail arterial rings were investigated after culture. Rings were cultured for 4 days in the absence and presence of 10% FCS and then mounted for physiological experiments. Intracellular Ca(2+) concentration ([Ca(2+)](i)) after stimulation with norepinephrine was similar in rings cultured with and without FCS, whereas force development after FCS was decreased by >50%. The difference persisted after permeabilization with beta-escin. These effects were associated with the presence of vasoconstrictors in FCS and were dissociated from its growth-stimulatory action. FCS treatment increased lactate production but did not affect ATP, ADP, or AMP contents. The contents of actin and myosin were decreased by culture but similar for all culture conditions. There was no effect of FCS on calponin contents or myosin SM1/SM2 isoform composition, nor was there any appearance of nonmuscle myosin. FCS-stimulated rings showed evidence of cell degeneration not found after culture without FCS or with FCS + verapamil (1 microM) to lower [Ca(2+)](i). The decreased force-generating ability after culture with FCS is thus associated with increased [Ca(2+)](i) during culture and not primarily caused by growth-associated modulation of cells from the contractile to the synthetic phenotype. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
tail artery, organ culture, contraction, calcium, ultrastructure
in
American Journal of Physiology
volume
277
issue
1
pages
64 - 73
publisher
American Pysiological Society
external identifiers
  • pmid:10409109
ISSN
0002-9513
language
English
LU publication?
yes
id
85680bf1-bb96-40ee-b201-72750692b2de (old id 1115258)
alternative location
http://ajpcell.physiology.org/cgi/content/full/277/1/C64
date added to LUP
2008-07-07 16:27:39
date last changed
2016-04-16 03:26:30
@article{85680bf1-bb96-40ee-b201-72750692b2de,
  abstract     = {Culture of dispersed smooth muscle cells is known to cause rapid modulation from the contractile to the synthetic cellular phenotype. However, organ culture of smooth muscle tissue, with maintained extracellular matrix and cell-cell contacts, may facilitate maintenance of the contractile phenotype. To test the influence of culture conditions, structural, functional, and biochemical properties of rat tail arterial rings were investigated after culture. Rings were cultured for 4 days in the absence and presence of 10% FCS and then mounted for physiological experiments. Intracellular Ca(2+) concentration ([Ca(2+)](i)) after stimulation with norepinephrine was similar in rings cultured with and without FCS, whereas force development after FCS was decreased by >50%. The difference persisted after permeabilization with beta-escin. These effects were associated with the presence of vasoconstrictors in FCS and were dissociated from its growth-stimulatory action. FCS treatment increased lactate production but did not affect ATP, ADP, or AMP contents. The contents of actin and myosin were decreased by culture but similar for all culture conditions. There was no effect of FCS on calponin contents or myosin SM1/SM2 isoform composition, nor was there any appearance of nonmuscle myosin. FCS-stimulated rings showed evidence of cell degeneration not found after culture without FCS or with FCS + verapamil (1 microM) to lower [Ca(2+)](i). The decreased force-generating ability after culture with FCS is thus associated with increased [Ca(2+)](i) during culture and not primarily caused by growth-associated modulation of cells from the contractile to the synthetic phenotype.},
  author       = {Lindqvist, Anders and Nordström, Ina and Malmqvist, Ulf and Nordenfelt, Patrik and Hellstrand, Per},
  issn         = {0002-9513},
  keyword      = {tail artery,organ culture,contraction,calcium,ultrastructure},
  language     = {eng},
  number       = {1},
  pages        = {64--73},
  publisher    = {American Pysiological Society},
  series       = {American Journal of Physiology},
  title        = {Long-term effects of Ca(2+) on structure and contractility of vascular smooth muscle},
  volume       = {277},
  year         = {1999},
}