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The homeodomain transcription factors Islet 1 and HB9 are expressed in adult alpha and gamma motoneurons identified by selective retrograde tracing

Vult von Steyern, Fredrik LU ; Martinov, V; Rabben, I; Nja, A; de Lapeyriere, O and Lomo, T (1999) In European Journal of Neuroscience 11(6). p.2093-2102
Abstract
To study gene expression in differentiated adult motoneuron subtypes, we used fluorescent dextrans for both anterograde and retrograde axonal tracing in adult rat and mouse. Application of these dyes to the cut distal and proximal ends of small extramuscular nerve branches revealed both the peripheral ramifications and the cell bodies of subsets of motoneurons. We show that the soleus muscle is innervated by two nerve branches, one of which contains gamma motor and sensory axons but no alpha motor axons. By retrograde tracing of this branch, we selectively labelled gamma motoneurons. In adult rat, the nerves innervating the soleus and extensor digitorum longus muscles contain almost exclusively axons innervating slow (type I) and fast... (More)
To study gene expression in differentiated adult motoneuron subtypes, we used fluorescent dextrans for both anterograde and retrograde axonal tracing in adult rat and mouse. Application of these dyes to the cut distal and proximal ends of small extramuscular nerve branches revealed both the peripheral ramifications and the cell bodies of subsets of motoneurons. We show that the soleus muscle is innervated by two nerve branches, one of which contains gamma motor and sensory axons but no alpha motor axons. By retrograde tracing of this branch, we selectively labelled gamma motoneurons. In adult rat, the nerves innervating the soleus and extensor digitorum longus muscles contain almost exclusively axons innervating slow (type I) and fast (type 2) muscle fibres, respectively. We selectively labelled slow and fast type motoneurons by retrograde tracing of these nerves. With immunocytochemistry we show that adult motoneurons express several homeodomain genes that are associated with motoneuron differentiation during early embryonic development. Combining selective retrograde labelling with immunocytochemistry we compared the expression patterns in alpha and gamma motoneurons. The homeodomain transcription factors Islet 1 and HB9 were expressed in slow and fast alpha motoneurons and in soleus gamma motoneurons. Motoneurons in each population varied in their intensity of the immunostaining, but no factor or combination of factors was unique to any one population. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
axonal tracing, fluorescent dextran, gene expression, mouse, rat
in
European Journal of Neuroscience
volume
11
issue
6
pages
2093 - 2102
publisher
Wiley-Blackwell
external identifiers
  • pmid:10336678
  • scopus:0033006664
ISSN
1460-9568
language
English
LU publication?
yes
id
bd421ea9-1b7d-4b3e-8804-b4cc63c213b1 (old id 1115672)
date added to LUP
2008-07-08 11:26:48
date last changed
2017-02-12 03:36:51
@article{bd421ea9-1b7d-4b3e-8804-b4cc63c213b1,
  abstract     = {To study gene expression in differentiated adult motoneuron subtypes, we used fluorescent dextrans for both anterograde and retrograde axonal tracing in adult rat and mouse. Application of these dyes to the cut distal and proximal ends of small extramuscular nerve branches revealed both the peripheral ramifications and the cell bodies of subsets of motoneurons. We show that the soleus muscle is innervated by two nerve branches, one of which contains gamma motor and sensory axons but no alpha motor axons. By retrograde tracing of this branch, we selectively labelled gamma motoneurons. In adult rat, the nerves innervating the soleus and extensor digitorum longus muscles contain almost exclusively axons innervating slow (type I) and fast (type 2) muscle fibres, respectively. We selectively labelled slow and fast type motoneurons by retrograde tracing of these nerves. With immunocytochemistry we show that adult motoneurons express several homeodomain genes that are associated with motoneuron differentiation during early embryonic development. Combining selective retrograde labelling with immunocytochemistry we compared the expression patterns in alpha and gamma motoneurons. The homeodomain transcription factors Islet 1 and HB9 were expressed in slow and fast alpha motoneurons and in soleus gamma motoneurons. Motoneurons in each population varied in their intensity of the immunostaining, but no factor or combination of factors was unique to any one population.},
  author       = {Vult von Steyern, Fredrik and Martinov, V and Rabben, I and Nja, A and de Lapeyriere, O and Lomo, T},
  issn         = {1460-9568},
  keyword      = {axonal tracing,fluorescent dextran,gene expression,mouse,rat},
  language     = {eng},
  number       = {6},
  pages        = {2093--2102},
  publisher    = {Wiley-Blackwell},
  series       = {European Journal of Neuroscience},
  title        = {The homeodomain transcription factors Islet 1 and HB9 are expressed in adult alpha and gamma motoneurons identified by selective retrograde tracing},
  volume       = {11},
  year         = {1999},
}