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Macrophage inflammatory protein-2 is required for neutrophil passage across the epithelial barrier of the infected urinary tract

Hang, Long; Haraoka, Masashi; Agace, William LU ; Leffler, Hakon LU ; Burdick, Marie; Strieter, Robert and Svanborg, Catharina LU (1999) In Journal of Immunology 162(5). p.3037-3044
Abstract
IL-8 is a major human neutrophil chemoattractant at mucosal infection sites. This study examined the C-X-C chemokine response to mucosal infection, and, specifically, the role of macrophage inflammatory protein (MIP)-2, one of the mouse IL-8 equivalents, for neutrophil-epithelial interactions. Following intravesical Escherichia coli infection, several C-X-C chemokines were secreted into the urine, but only MIP-2 concentrations correlated to neutrophil numbers. Tissue quantitation demonstrated that kidney MIP-2 production was triggered by infection, and immunohistochemistry identified the kidney epithelium as a main source of MIP-2. Treatment with anti-MIP-2 Ab reduced the urine neutrophil numbers, but the mice had normal tissue neutrophil... (More)
IL-8 is a major human neutrophil chemoattractant at mucosal infection sites. This study examined the C-X-C chemokine response to mucosal infection, and, specifically, the role of macrophage inflammatory protein (MIP)-2, one of the mouse IL-8 equivalents, for neutrophil-epithelial interactions. Following intravesical Escherichia coli infection, several C-X-C chemokines were secreted into the urine, but only MIP-2 concentrations correlated to neutrophil numbers. Tissue quantitation demonstrated that kidney MIP-2 production was triggered by infection, and immunohistochemistry identified the kidney epithelium as a main source of MIP-2. Treatment with anti-MIP-2 Ab reduced the urine neutrophil numbers, but the mice had normal tissue neutrophil levels. By immunohistochemistry, the neutrophils were found in aggregates under the pelvic epithelium, but in control mice the neutrophils crossed the urothelium into the urine. The results demonstrate that different chemokines direct neutrophil migration from the bloodstream to the lamina propria and across the epithelium and that MIP-2 serves the latter function. These findings suggest that neutrophils cross epithelial cell barriers in a highly regulated manner in response to chemokines elaborated at this site. This is yet another mechanism that defines the mucosal compartment and differentiates the local from the systemic host response. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Journal of Immunology
volume
162
issue
5
pages
3037 - 3044
publisher
American Association of Immunologists
external identifiers
  • pmid:10072556
  • scopus:0033104826
ISSN
1550-6606
language
English
LU publication?
yes
id
f1067d98-4a6e-4e5b-b8f9-f7cf90262538 (old id 1115679)
alternative location
http://www.jimmunol.org/cgi/content/full/162/5/3037
date added to LUP
2008-07-08 11:32:22
date last changed
2017-08-06 04:38:32
@article{f1067d98-4a6e-4e5b-b8f9-f7cf90262538,
  abstract     = {IL-8 is a major human neutrophil chemoattractant at mucosal infection sites. This study examined the C-X-C chemokine response to mucosal infection, and, specifically, the role of macrophage inflammatory protein (MIP)-2, one of the mouse IL-8 equivalents, for neutrophil-epithelial interactions. Following intravesical Escherichia coli infection, several C-X-C chemokines were secreted into the urine, but only MIP-2 concentrations correlated to neutrophil numbers. Tissue quantitation demonstrated that kidney MIP-2 production was triggered by infection, and immunohistochemistry identified the kidney epithelium as a main source of MIP-2. Treatment with anti-MIP-2 Ab reduced the urine neutrophil numbers, but the mice had normal tissue neutrophil levels. By immunohistochemistry, the neutrophils were found in aggregates under the pelvic epithelium, but in control mice the neutrophils crossed the urothelium into the urine. The results demonstrate that different chemokines direct neutrophil migration from the bloodstream to the lamina propria and across the epithelium and that MIP-2 serves the latter function. These findings suggest that neutrophils cross epithelial cell barriers in a highly regulated manner in response to chemokines elaborated at this site. This is yet another mechanism that defines the mucosal compartment and differentiates the local from the systemic host response.},
  author       = {Hang, Long and Haraoka, Masashi and Agace, William and Leffler, Hakon and Burdick, Marie and Strieter, Robert and Svanborg, Catharina},
  issn         = {1550-6606},
  language     = {eng},
  number       = {5},
  pages        = {3037--3044},
  publisher    = {American Association of Immunologists},
  series       = {Journal of Immunology},
  title        = {Macrophage inflammatory protein-2 is required for neutrophil passage across the epithelial barrier of the infected urinary tract},
  volume       = {162},
  year         = {1999},
}