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Glandular kallikreins of the cotton-top tamarin: molecular cloning of the gene encoding the tissue kallikrein

Ceder, Yvonne LU ; Persson, Margareta LU ; Valtonen-André, Camilla LU and Lundwall, Åke LU (2000) In DNA and Cell Biology 19(12). p.721-727
Abstract
The glandular kallikrein family is composed of structurally related serine proteases. Studies show that the mouse family encompasses at least 14 highly conserved functional genes, but of these only the tissue kallikarein has a human ortholog. In man, the tissue kallikrein display high sequence similarity with prostate specific antigen and human glandular kallikrein 2, suggesting that they evolved after the separation of primates and rodents. A phylogenetic study of the genes encoding glandular kallikreins in species evolutionarily located between rodents and man may reveal interesting details on how the gene family evolved, which in turn could yield information about the function of the proteins. Therefore, we have initiated a study of the... (More)
The glandular kallikrein family is composed of structurally related serine proteases. Studies show that the mouse family encompasses at least 14 highly conserved functional genes, but of these only the tissue kallikarein has a human ortholog. In man, the tissue kallikrein display high sequence similarity with prostate specific antigen and human glandular kallikrein 2, suggesting that they evolved after the separation of primates and rodents. A phylogenetic study of the genes encoding glandular kallikreins in species evolutionarily located between rodents and man may reveal interesting details on how the gene family evolved, which in turn could yield information about the function of the proteins. Therefore, we have initiated a study of the glandular kallikreins of the cotton-top tamarin (Saguinus oedipus), a New World Monkey. Here, we report the cloning and nucleotide sequence of one of these, the tissue kallikrein gene. The gene of 4.4 kb is composed of five exons, and the structure is 90% similar to that of the orthologous human gene. It gives rise to a polypeptide of 261 amino acids, including a signal peptide of 17 residues, a pro-piece of 7 residues, and the mature protein of 237 residues with an estimated molecular mass of 26.3 kD. The similarity to the human prostate specific antigen and human glandular kallikrein 2 genes is 73% and 72%, respectively, including introns and flanking regions. The lower similarity to these genes compared with the human tissue kallikrein gene indicates that they, or a progenitor to them, arose in primates prior to the separation of New and Old World monkeys. Genomic Southern blots also show that the cotton-top tamarin genome encompasses at least one more glandular kallikrein gene. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
DNA and Cell Biology
volume
19
issue
12
pages
721 - 727
publisher
Mary Ann Liebert, Inc.
external identifiers
  • pmid:11177570
  • scopus:0034529145
ISSN
1044-5498
DOI
10.1089/104454900750058080
language
English
LU publication?
yes
id
e31bccc8-d110-4a0f-9644-8469380f3d02 (old id 1116271)
date added to LUP
2008-07-01 11:06:39
date last changed
2017-01-01 07:28:52
@article{e31bccc8-d110-4a0f-9644-8469380f3d02,
  abstract     = {The glandular kallikrein family is composed of structurally related serine proteases. Studies show that the mouse family encompasses at least 14 highly conserved functional genes, but of these only the tissue kallikarein has a human ortholog. In man, the tissue kallikrein display high sequence similarity with prostate specific antigen and human glandular kallikrein 2, suggesting that they evolved after the separation of primates and rodents. A phylogenetic study of the genes encoding glandular kallikreins in species evolutionarily located between rodents and man may reveal interesting details on how the gene family evolved, which in turn could yield information about the function of the proteins. Therefore, we have initiated a study of the glandular kallikreins of the cotton-top tamarin (Saguinus oedipus), a New World Monkey. Here, we report the cloning and nucleotide sequence of one of these, the tissue kallikrein gene. The gene of 4.4 kb is composed of five exons, and the structure is 90% similar to that of the orthologous human gene. It gives rise to a polypeptide of 261 amino acids, including a signal peptide of 17 residues, a pro-piece of 7 residues, and the mature protein of 237 residues with an estimated molecular mass of 26.3 kD. The similarity to the human prostate specific antigen and human glandular kallikrein 2 genes is 73% and 72%, respectively, including introns and flanking regions. The lower similarity to these genes compared with the human tissue kallikrein gene indicates that they, or a progenitor to them, arose in primates prior to the separation of New and Old World monkeys. Genomic Southern blots also show that the cotton-top tamarin genome encompasses at least one more glandular kallikrein gene.},
  author       = {Ceder, Yvonne and Persson, Margareta and Valtonen-André, Camilla and Lundwall, Åke},
  issn         = {1044-5498},
  language     = {eng},
  number       = {12},
  pages        = {721--727},
  publisher    = {Mary Ann Liebert, Inc.},
  series       = {DNA and Cell Biology},
  title        = {Glandular kallikreins of the cotton-top tamarin: molecular cloning of the gene encoding the tissue kallikrein},
  url          = {http://dx.doi.org/10.1089/104454900750058080},
  volume       = {19},
  year         = {2000},
}