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pUB2380: characterization of a ColD-like resistance plasmid

Albiger, Barbara LU ; Comanducci, A; Dodd, H M; Tavakoli, N; Lett, M C and Bennett, P (2000) In Plasmid 44(1). p.54-65
Abstract
A detailed analysis of the mobilizable, ColE1-like resistance plasmid, pUB2380, is reported. The 8.5-kb genome encodes six (possibly seven) major functions: (1) a ColD-like origin of replication, oriV, with associated replication functions, RNAI and RNAII; (2) a set of active mobilization functions highly homologous to that of ColE1, including the origin of transfer, oriT; (3) a ColE1-like multimer resolution site (cer); (4) a kanamycin-resistance determinant, aph, encoding an aminoglycoside-3'-phosphotransferase type 1; (5) an insertion sequence, IS1294; and (6) two genes, probably cotranscribed, of unknown function(s). The GC content of the various parts of the genome indicates that the plasmid is a hybrid structure assembled from DNA... (More)
A detailed analysis of the mobilizable, ColE1-like resistance plasmid, pUB2380, is reported. The 8.5-kb genome encodes six (possibly seven) major functions: (1) a ColD-like origin of replication, oriV, with associated replication functions, RNAI and RNAII; (2) a set of active mobilization functions highly homologous to that of ColE1, including the origin of transfer, oriT; (3) a ColE1-like multimer resolution site (cer); (4) a kanamycin-resistance determinant, aph, encoding an aminoglycoside-3'-phosphotransferase type 1; (5) an insertion sequence, IS1294; and (6) two genes, probably cotranscribed, of unknown function(s). The GC content of the various parts of the genome indicates that the plasmid is a hybrid structure assembled from DNA from at least three different sources, of which the replication region, the mobilization functions, and the resistance gene are likely to have originated in the enterobacteriaceae. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Plasmid
volume
44
issue
1
pages
54 - 65
publisher
Academic Press
external identifiers
  • pmid:10873527
  • scopus:0033911703
ISSN
0147-619X
DOI
10.1006/plas.1999.1459
language
English
LU publication?
no
id
833de712-2fc6-449e-93b1-a96e8d1e4b40 (old id 1116307)
date added to LUP
2008-07-01 11:27:39
date last changed
2017-01-01 04:38:50
@article{833de712-2fc6-449e-93b1-a96e8d1e4b40,
  abstract     = {A detailed analysis of the mobilizable, ColE1-like resistance plasmid, pUB2380, is reported. The 8.5-kb genome encodes six (possibly seven) major functions: (1) a ColD-like origin of replication, oriV, with associated replication functions, RNAI and RNAII; (2) a set of active mobilization functions highly homologous to that of ColE1, including the origin of transfer, oriT; (3) a ColE1-like multimer resolution site (cer); (4) a kanamycin-resistance determinant, aph, encoding an aminoglycoside-3'-phosphotransferase type 1; (5) an insertion sequence, IS1294; and (6) two genes, probably cotranscribed, of unknown function(s). The GC content of the various parts of the genome indicates that the plasmid is a hybrid structure assembled from DNA from at least three different sources, of which the replication region, the mobilization functions, and the resistance gene are likely to have originated in the enterobacteriaceae.},
  author       = {Albiger, Barbara and Comanducci, A and Dodd, H M and Tavakoli, N and Lett, M C and Bennett, P},
  issn         = {0147-619X},
  language     = {eng},
  number       = {1},
  pages        = {54--65},
  publisher    = {Academic Press},
  series       = {Plasmid},
  title        = {pUB2380: characterization of a ColD-like resistance plasmid},
  url          = {http://dx.doi.org/10.1006/plas.1999.1459},
  volume       = {44},
  year         = {2000},
}