Advanced

Human intestinal lamina propria and intraepithelial lymphocytes express receptors specific for chemokines induced by inflammation

Agace, William LU ; Roberts, A I; Wu, L; Greineder, C; Ebert, E C and Parker, C M (2000) In European Journal of Immunology 30(3). p.819-826
Abstract
To determine which chemokine receptors might be involved in T lymphocyte localization to the intestinal mucosa, we examined receptor expression on human intestinal lamina propria lymphocytes (LPL), intraepithelial lymphocytes (IEL) and CD45RO+beta7hi gut homing peripheral blood lymphocytes (PBL). Virtually all LPL and IEL expressed CXCR3 and CCR5, receptors that have been associated with Th1(Tc1)/Th0 lymphocytes, while CCR3 and CCR4, receptors associated with Th2 (Tc2)lymphocytes, CCR7, CXCR1 and CXCR2 were not expressed. CXCR3 and CCR5 receptors were functional, as LPL and IEL migrated to their respective ligands I-TAC and RANTES. In addition, most alphaEbeta7- LPL and IEL expressed high levels of CCR2. While the majority of... (More)
To determine which chemokine receptors might be involved in T lymphocyte localization to the intestinal mucosa, we examined receptor expression on human intestinal lamina propria lymphocytes (LPL), intraepithelial lymphocytes (IEL) and CD45RO+beta7hi gut homing peripheral blood lymphocytes (PBL). Virtually all LPL and IEL expressed CXCR3 and CCR5, receptors that have been associated with Th1(Tc1)/Th0 lymphocytes, while CCR3 and CCR4, receptors associated with Th2 (Tc2)lymphocytes, CCR7, CXCR1 and CXCR2 were not expressed. CXCR3 and CCR5 receptors were functional, as LPL and IEL migrated to their respective ligands I-TAC and RANTES. In addition, most alphaEbeta7- LPL and IEL expressed high levels of CCR2. While the majority of CD45RO(-)beta7hi PBL also expressed CXCR3 and CCR5, a proportion of these cells were CXCR3- and/or CCR5- and some expressed CCR4 and/or CCR7, indicating that lymphocytes recruited to the intestinal mucosa represent a subset of these cells. In summary, our results show that LPL and IEL within the normal intestine express a specific and similar array of chemokine receptors whose ligands are constitutively expressed in the intestinal mucosa and whose expression is up-regulated during intestinal inflammation. These results support the view that CXCR3, CCR5 and CCR2 may play an important role in lymphocyte localization within the intestinal mucosa. (Less)
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Mucosa, Chemokine, T lymphocyte, Homing, Epithelium
in
European Journal of Immunology
volume
30
issue
3
pages
819 - 826
publisher
John Wiley & Sons
external identifiers
  • pmid:10741397
  • scopus:0034001778
ISSN
1521-4141
DOI
10.1002/1521-4141(200003)30:3<819::AID-IMMU819>3.0.CO;2-Y
language
English
LU publication?
no
id
82ebd946-d056-40e9-b7b1-b6b9a45b73ac (old id 1116360)
date added to LUP
2008-07-01 12:08:07
date last changed
2017-08-06 03:34:39
@article{82ebd946-d056-40e9-b7b1-b6b9a45b73ac,
  abstract     = {To determine which chemokine receptors might be involved in T lymphocyte localization to the intestinal mucosa, we examined receptor expression on human intestinal lamina propria lymphocytes (LPL), intraepithelial lymphocytes (IEL) and CD45RO+beta7hi gut homing peripheral blood lymphocytes (PBL). Virtually all LPL and IEL expressed CXCR3 and CCR5, receptors that have been associated with Th1(Tc1)/Th0 lymphocytes, while CCR3 and CCR4, receptors associated with Th2 (Tc2)lymphocytes, CCR7, CXCR1 and CXCR2 were not expressed. CXCR3 and CCR5 receptors were functional, as LPL and IEL migrated to their respective ligands I-TAC and RANTES. In addition, most alphaEbeta7- LPL and IEL expressed high levels of CCR2. While the majority of CD45RO(-)beta7hi PBL also expressed CXCR3 and CCR5, a proportion of these cells were CXCR3- and/or CCR5- and some expressed CCR4 and/or CCR7, indicating that lymphocytes recruited to the intestinal mucosa represent a subset of these cells. In summary, our results show that LPL and IEL within the normal intestine express a specific and similar array of chemokine receptors whose ligands are constitutively expressed in the intestinal mucosa and whose expression is up-regulated during intestinal inflammation. These results support the view that CXCR3, CCR5 and CCR2 may play an important role in lymphocyte localization within the intestinal mucosa.},
  author       = {Agace, William and Roberts, A I and Wu, L and Greineder, C and Ebert, E C and Parker, C M},
  issn         = {1521-4141},
  keyword      = {Mucosa,Chemokine,T lymphocyte,Homing,Epithelium},
  language     = {eng},
  number       = {3},
  pages        = {819--826},
  publisher    = {John Wiley & Sons},
  series       = {European Journal of Immunology},
  title        = {Human intestinal lamina propria and intraepithelial lymphocytes express receptors specific for chemokines induced by inflammation},
  url          = {http://dx.doi.org/10.1002/1521-4141(200003)30:3<819::AID-IMMU819>3.0.CO;2-Y},
  volume       = {30},
  year         = {2000},
}