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Gene variation of 5'-NCR and core region in serum samples collected from patients with HCV infection

Zhang, S; Zhuang, H; Li, H; Widell, Anders LU ; Qi, Z and Zhang, H (2000) In Zhonghua gan zang bing za zhi. Chinese journal of hepatology 8(3). p.144-146
Abstract
OBJECTIVE: To study the variation of HCV 5'-NCR and core gene in serial sera collected from patients with HCV infection. METHODS: Serial sera were collected from 4 plasma-donors with HCV infection. 5'-NCR and core gene were amplified, sequenced and analyzed using software. RESULTS: All the sequences obtained from the serial sera of patients with HCV infection were 1b and 2a subtypes. The variation of 5'-NCR only related to the genotype, and not associated with patients and time of serum collection. However, the sequences of core gene were not identical in HCV strains isolated from different patients. The sequences of those two regions of the same genotype isolated from the same patient did not change with time. CONCLUSION: 5'-NCR is more... (More)
OBJECTIVE: To study the variation of HCV 5'-NCR and core gene in serial sera collected from patients with HCV infection. METHODS: Serial sera were collected from 4 plasma-donors with HCV infection. 5'-NCR and core gene were amplified, sequenced and analyzed using software. RESULTS: All the sequences obtained from the serial sera of patients with HCV infection were 1b and 2a subtypes. The variation of 5'-NCR only related to the genotype, and not associated with patients and time of serum collection. However, the sequences of core gene were not identical in HCV strains isolated from different patients. The sequences of those two regions of the same genotype isolated from the same patient did not change with time. CONCLUSION: 5'-NCR is more conserved than the core region and the genotype is the major cause of gene variation. No change in sequences of those two regions is found at the different points of time. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Zhonghua gan zang bing za zhi. Chinese journal of hepatology
volume
8
issue
3
pages
144 - 146
publisher
Chongqing yi ke da xue, di 2 lin chuang xue yuan Bing du xing gan yan yan jiu suo
external identifiers
  • pmid:10880160
  • scopus:0034203114
ISSN
1007-3418
language
Chinese
LU publication?
yes
id
808098db-0a44-480e-94a4-b576bd5cf85e (old id 1116429)
date added to LUP
2008-07-01 13:03:20
date last changed
2017-01-01 07:23:59
@article{808098db-0a44-480e-94a4-b576bd5cf85e,
  abstract     = {OBJECTIVE: To study the variation of HCV 5'-NCR and core gene in serial sera collected from patients with HCV infection. METHODS: Serial sera were collected from 4 plasma-donors with HCV infection. 5'-NCR and core gene were amplified, sequenced and analyzed using software. RESULTS: All the sequences obtained from the serial sera of patients with HCV infection were 1b and 2a subtypes. The variation of 5'-NCR only related to the genotype, and not associated with patients and time of serum collection. However, the sequences of core gene were not identical in HCV strains isolated from different patients. The sequences of those two regions of the same genotype isolated from the same patient did not change with time. CONCLUSION: 5'-NCR is more conserved than the core region and the genotype is the major cause of gene variation. No change in sequences of those two regions is found at the different points of time.},
  author       = {Zhang, S and Zhuang, H and Li, H and Widell, Anders and Qi, Z and Zhang, H},
  issn         = {1007-3418},
  language     = {chi},
  number       = {3},
  pages        = {144--146},
  publisher    = {Chongqing yi ke da xue, di 2 lin chuang xue yuan Bing du xing gan yan yan jiu suo},
  series       = {Zhonghua gan zang bing za zhi. Chinese journal of hepatology},
  title        = {Gene variation of 5'-NCR and core region in serum samples collected from patients with HCV infection},
  volume       = {8},
  year         = {2000},
}