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Mixed infection with two types of hepatitis C virus is probably a rare event

Viazov, S; Widell, Anders LU and Nordenfelt, E (2000) In Infection 28(1). p.21-25
Abstract
A search for the simultaneous presence of two hepatitis C virus (HCV) types in sera of a group of chronically infected intravenous drug users, hemodialysis patients and hemophiliacs from Sweden and Russia was performed with two genotyping methods based on the use of type-specific primers from core and NS4 regions of the viral genome. An important feature of NS4 based assay is that type-specific primers are used in both rounds of nested PCR, thus providing the possibility of the identification not only of the abundant type, but also of the minor HCV type present in a particular serum. The experiments, however, did not reveal the simultaneous presence of two or more HCV types in any of the 40 samples. These results suggest that the frequency... (More)
A search for the simultaneous presence of two hepatitis C virus (HCV) types in sera of a group of chronically infected intravenous drug users, hemodialysis patients and hemophiliacs from Sweden and Russia was performed with two genotyping methods based on the use of type-specific primers from core and NS4 regions of the viral genome. An important feature of NS4 based assay is that type-specific primers are used in both rounds of nested PCR, thus providing the possibility of the identification not only of the abundant type, but also of the minor HCV type present in a particular serum. The experiments, however, did not reveal the simultaneous presence of two or more HCV types in any of the 40 samples. These results suggest that the frequency of mixed infections in serum with different HCV types is very low even in high-risk groups, at least in the geographic region studied. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
HCV genotypes - Mixed infection
in
Infection
volume
28
issue
1
pages
21 - 25
publisher
Urban & Vogel
external identifiers
  • pmid:10697786
  • scopus:0033950033
ISSN
1439-0973
DOI
10.1007/s150100050005
language
English
LU publication?
yes
id
74f7eb15-5940-4894-8c44-9c65c2681b01 (old id 1116452)
date added to LUP
2008-07-01 13:16:01
date last changed
2017-01-15 04:11:34
@article{74f7eb15-5940-4894-8c44-9c65c2681b01,
  abstract     = {A search for the simultaneous presence of two hepatitis C virus (HCV) types in sera of a group of chronically infected intravenous drug users, hemodialysis patients and hemophiliacs from Sweden and Russia was performed with two genotyping methods based on the use of type-specific primers from core and NS4 regions of the viral genome. An important feature of NS4 based assay is that type-specific primers are used in both rounds of nested PCR, thus providing the possibility of the identification not only of the abundant type, but also of the minor HCV type present in a particular serum. The experiments, however, did not reveal the simultaneous presence of two or more HCV types in any of the 40 samples. These results suggest that the frequency of mixed infections in serum with different HCV types is very low even in high-risk groups, at least in the geographic region studied.},
  author       = {Viazov, S and Widell, Anders and Nordenfelt, E},
  issn         = {1439-0973},
  keyword      = {HCV genotypes - Mixed infection},
  language     = {eng},
  number       = {1},
  pages        = {21--25},
  publisher    = {Urban & Vogel},
  series       = {Infection},
  title        = {Mixed infection with two types of hepatitis C virus is probably a rare event},
  url          = {http://dx.doi.org/10.1007/s150100050005},
  volume       = {28},
  year         = {2000},
}