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Changes in polyamine metabolism during glucocorticoid-induced programmed cell death in mouse thymus

Hegardt, Cecilia LU ; Andersson, G and Oredsson, Stina LU (2000) In Cell Biology International 24(12). p.871-880
Abstract
When mice are injected with dexamethasone, cortical thymocytes are deleted through programmed cell death (PCD). We have used this in vivo model system to investigate the kinetics of PCD and cell proliferation in relation to polyamine metabolism for 16 h after injection of dexamethasone. As a marker for PCD, we used the appearance of a sub-G(1)peak in the DNA histogram. When a sub-G(1)peak appeared at 4 h after dexamethasone treatment, the activity of the polyamine catabolic enzyme spermidine/spermine N(1)-acetyltransferase (SSAT) was significantly increased and the activity of the polyamine biosynthetic enzyme S-adenosylmethionine decarboxylase (AdoMetDC) was significantly decreased compared to the activities found in the thymi of control... (More)
When mice are injected with dexamethasone, cortical thymocytes are deleted through programmed cell death (PCD). We have used this in vivo model system to investigate the kinetics of PCD and cell proliferation in relation to polyamine metabolism for 16 h after injection of dexamethasone. As a marker for PCD, we used the appearance of a sub-G(1)peak in the DNA histogram. When a sub-G(1)peak appeared at 4 h after dexamethasone treatment, the activity of the polyamine catabolic enzyme spermidine/spermine N(1)-acetyltransferase (SSAT) was significantly increased and the activity of the polyamine biosynthetic enzyme S-adenosylmethionine decarboxylase (AdoMetDC) was significantly decreased compared to the activities found in the thymi of control mice. Despite the significant changes in the activities of SSAT and AdoMetDC, the only change in the polyamine pool during the experimental period was that of putrescine. Presumably the complexity of this in vivo system masks changes in the spermidine and spermine pools that were expected in relation to the increased SSAT activity and decreased AdoMetDC activity. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
apoptosis, polyamines, thymus, glucocorticoid, flow cytometry
in
Cell Biology International
volume
24
issue
12
pages
871 - 880
publisher
Elsevier
external identifiers
  • pmid:11114236
  • scopus:0034522847
ISSN
1095-8355
DOI
10.1006/cbir.2000.0578
language
English
LU publication?
yes
id
07b2f0f5-6f33-4b71-b286-b74314543561 (old id 1116610)
date added to LUP
2008-07-02 09:12:58
date last changed
2017-01-01 04:59:34
@article{07b2f0f5-6f33-4b71-b286-b74314543561,
  abstract     = {When mice are injected with dexamethasone, cortical thymocytes are deleted through programmed cell death (PCD). We have used this in vivo model system to investigate the kinetics of PCD and cell proliferation in relation to polyamine metabolism for 16 h after injection of dexamethasone. As a marker for PCD, we used the appearance of a sub-G(1)peak in the DNA histogram. When a sub-G(1)peak appeared at 4 h after dexamethasone treatment, the activity of the polyamine catabolic enzyme spermidine/spermine N(1)-acetyltransferase (SSAT) was significantly increased and the activity of the polyamine biosynthetic enzyme S-adenosylmethionine decarboxylase (AdoMetDC) was significantly decreased compared to the activities found in the thymi of control mice. Despite the significant changes in the activities of SSAT and AdoMetDC, the only change in the polyamine pool during the experimental period was that of putrescine. Presumably the complexity of this in vivo system masks changes in the spermidine and spermine pools that were expected in relation to the increased SSAT activity and decreased AdoMetDC activity.},
  author       = {Hegardt, Cecilia and Andersson, G and Oredsson, Stina},
  issn         = {1095-8355},
  keyword      = {apoptosis,polyamines,thymus,glucocorticoid,flow cytometry},
  language     = {eng},
  number       = {12},
  pages        = {871--880},
  publisher    = {Elsevier},
  series       = {Cell Biology International},
  title        = {Changes in polyamine metabolism during glucocorticoid-induced programmed cell death in mouse thymus},
  url          = {http://dx.doi.org/10.1006/cbir.2000.0578},
  volume       = {24},
  year         = {2000},
}